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Investigation of the association between the GLC3A locus and normal tension glaucoma in Japanese patients by microsatellite analysis

PURPOSE: To investigate whether the GLC3A locus harboring the CYP1B1 gene is associated with normal tension glaucoma (NTG) in Japanese patients. MATERIALS AND METHODS: One hundred forty-two Japanese patients with NTG and 101 Japanese healthy controls were recruited. Patients exhibiting a comparative...

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Autores principales: Kamio, M, Meguro, A, Ota, M, Nomura, N, Kashiwagi, K, Mabuchi, F, Iijima, H, Kawase, K, Yamamoto, T, Nakamura, M, Negi, A, Sagara, T, Nishida, T, Inatani, M, Tanihara, H, Aihara, M, Araie, M, Fukuchi, T, Abe, H, Higashide, T, Sugiyama, K, Kanamoto, T, Kiuchi, Y, Iwase, A, Ohno, S, Inoko, H, Mizuki, N
Formato: Texto
Lenguaje:English
Publicado: Dove Medical Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2708983/
https://www.ncbi.nlm.nih.gov/pubmed/19668563
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author Kamio, M
Meguro, A
Ota, M
Nomura, N
Kashiwagi, K
Mabuchi, F
Iijima, H
Kawase, K
Yamamoto, T
Nakamura, M
Negi, A
Sagara, T
Nishida, T
Inatani, M
Tanihara, H
Aihara, M
Araie, M
Fukuchi, T
Abe, H
Higashide, T
Sugiyama, K
Kanamoto, T
Kiuchi, Y
Iwase, A
Ohno, S
Inoko, H
Mizuki, N
author_facet Kamio, M
Meguro, A
Ota, M
Nomura, N
Kashiwagi, K
Mabuchi, F
Iijima, H
Kawase, K
Yamamoto, T
Nakamura, M
Negi, A
Sagara, T
Nishida, T
Inatani, M
Tanihara, H
Aihara, M
Araie, M
Fukuchi, T
Abe, H
Higashide, T
Sugiyama, K
Kanamoto, T
Kiuchi, Y
Iwase, A
Ohno, S
Inoko, H
Mizuki, N
author_sort Kamio, M
collection PubMed
description PURPOSE: To investigate whether the GLC3A locus harboring the CYP1B1 gene is associated with normal tension glaucoma (NTG) in Japanese patients. MATERIALS AND METHODS: One hundred forty-two Japanese patients with NTG and 101 Japanese healthy controls were recruited. Patients exhibiting a comparatively early onset were selected as this suggests that genetic factors may show stronger involvement. Genotyping and assessment of allelic diversity was performed on 13 highly polymorphic microsatellite markers in and around the GLC3A locus. RESULTS: There were decreased frequencies of the 444 allele of D2S0416i and the 258 allele of D2S0425i in cases compared to controls (P = 0.022 and P = 0.034, respectively). However, this statistical significance disappeared when corrected (Pc > 0.05). We did not find any significant association between the remaining 11 microsatellite markers, including D2S177, which may be associated with CYP1B1, and NTG (P > 0.05). CONCLUSIONS: Our study showed no association between the GLCA3 locus and NTG, suggesting that the CYP1B1 gene, which is reportedly involved in a range of glaucoma phenotypes, may not be an associated factor in the pathogenesis of NTG.
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spelling pubmed-27089832009-08-10 Investigation of the association between the GLC3A locus and normal tension glaucoma in Japanese patients by microsatellite analysis Kamio, M Meguro, A Ota, M Nomura, N Kashiwagi, K Mabuchi, F Iijima, H Kawase, K Yamamoto, T Nakamura, M Negi, A Sagara, T Nishida, T Inatani, M Tanihara, H Aihara, M Araie, M Fukuchi, T Abe, H Higashide, T Sugiyama, K Kanamoto, T Kiuchi, Y Iwase, A Ohno, S Inoko, H Mizuki, N Clin Ophthalmol Original Research PURPOSE: To investigate whether the GLC3A locus harboring the CYP1B1 gene is associated with normal tension glaucoma (NTG) in Japanese patients. MATERIALS AND METHODS: One hundred forty-two Japanese patients with NTG and 101 Japanese healthy controls were recruited. Patients exhibiting a comparatively early onset were selected as this suggests that genetic factors may show stronger involvement. Genotyping and assessment of allelic diversity was performed on 13 highly polymorphic microsatellite markers in and around the GLC3A locus. RESULTS: There were decreased frequencies of the 444 allele of D2S0416i and the 258 allele of D2S0425i in cases compared to controls (P = 0.022 and P = 0.034, respectively). However, this statistical significance disappeared when corrected (Pc > 0.05). We did not find any significant association between the remaining 11 microsatellite markers, including D2S177, which may be associated with CYP1B1, and NTG (P > 0.05). CONCLUSIONS: Our study showed no association between the GLCA3 locus and NTG, suggesting that the CYP1B1 gene, which is reportedly involved in a range of glaucoma phenotypes, may not be an associated factor in the pathogenesis of NTG. Dove Medical Press 2009 2009-06-02 /pmc/articles/PMC2708983/ /pubmed/19668563 Text en © 2009 Kamio et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Original Research
Kamio, M
Meguro, A
Ota, M
Nomura, N
Kashiwagi, K
Mabuchi, F
Iijima, H
Kawase, K
Yamamoto, T
Nakamura, M
Negi, A
Sagara, T
Nishida, T
Inatani, M
Tanihara, H
Aihara, M
Araie, M
Fukuchi, T
Abe, H
Higashide, T
Sugiyama, K
Kanamoto, T
Kiuchi, Y
Iwase, A
Ohno, S
Inoko, H
Mizuki, N
Investigation of the association between the GLC3A locus and normal tension glaucoma in Japanese patients by microsatellite analysis
title Investigation of the association between the GLC3A locus and normal tension glaucoma in Japanese patients by microsatellite analysis
title_full Investigation of the association between the GLC3A locus and normal tension glaucoma in Japanese patients by microsatellite analysis
title_fullStr Investigation of the association between the GLC3A locus and normal tension glaucoma in Japanese patients by microsatellite analysis
title_full_unstemmed Investigation of the association between the GLC3A locus and normal tension glaucoma in Japanese patients by microsatellite analysis
title_short Investigation of the association between the GLC3A locus and normal tension glaucoma in Japanese patients by microsatellite analysis
title_sort investigation of the association between the glc3a locus and normal tension glaucoma in japanese patients by microsatellite analysis
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2708983/
https://www.ncbi.nlm.nih.gov/pubmed/19668563
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