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Expression profiling of rice cultivars differing in their tolerance to long-term drought stress
Understanding the molecular basis of plant performance under water-limiting conditions will help to breed crop plants with a lower water demand. We investigated the physiological and gene expression response of drought-tolerant (IR57311 and LC-93-4) and drought-sensitive (Nipponbare and Taipei 309)...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Springer Netherlands
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2709230/ https://www.ncbi.nlm.nih.gov/pubmed/18931976 http://dx.doi.org/10.1007/s11103-008-9412-7 |
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author | Degenkolbe, Thomas Do, Phuc Thi Zuther, Ellen Repsilber, Dirk Walther, Dirk Hincha, Dirk K. Köhl, Karin I. |
author_facet | Degenkolbe, Thomas Do, Phuc Thi Zuther, Ellen Repsilber, Dirk Walther, Dirk Hincha, Dirk K. Köhl, Karin I. |
author_sort | Degenkolbe, Thomas |
collection | PubMed |
description | Understanding the molecular basis of plant performance under water-limiting conditions will help to breed crop plants with a lower water demand. We investigated the physiological and gene expression response of drought-tolerant (IR57311 and LC-93-4) and drought-sensitive (Nipponbare and Taipei 309) rice (Oryza sativa L.) cultivars to 18 days of drought stress in climate chamber experiments. Drought stressed plants grew significantly slower than the controls. Gene expression profiles were measured in leaf samples with the 20 K NSF oligonucleotide microarray. A linear model was fitted to the data to identify genes that were significantly regulated under drought stress. In all drought stressed cultivars, 245 genes were significantly repressed and 413 genes induced. Genes differing in their expression pattern under drought stress between tolerant and sensitive cultivars were identified by the genotype × environment (G × E) interaction term. More genes were significantly drought regulated in the sensitive than in the tolerant cultivars. Localizing all expressed genes on the rice genome map, we checked which genes with a significant G × E interaction co-localized with published quantitative trait loci regions for drought tolerance. These genes are more likely to be important for drought tolerance in an agricultural environment. To identify the metabolic processes with a significant G × E effect, we adapted the analysis software MapMan for rice. We found a drought stress induced shift toward senescence related degradation processes that was more pronounced in the sensitive than in the tolerant cultivars. In spite of higher growth rates and water use, more photosynthesis related genes were down-regulated in the tolerant than in the sensitive cultivars. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11103-008-9412-7) contains supplementary material, which is available to authorized users. |
format | Text |
id | pubmed-2709230 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-27092302009-07-14 Expression profiling of rice cultivars differing in their tolerance to long-term drought stress Degenkolbe, Thomas Do, Phuc Thi Zuther, Ellen Repsilber, Dirk Walther, Dirk Hincha, Dirk K. Köhl, Karin I. Plant Mol Biol Article Understanding the molecular basis of plant performance under water-limiting conditions will help to breed crop plants with a lower water demand. We investigated the physiological and gene expression response of drought-tolerant (IR57311 and LC-93-4) and drought-sensitive (Nipponbare and Taipei 309) rice (Oryza sativa L.) cultivars to 18 days of drought stress in climate chamber experiments. Drought stressed plants grew significantly slower than the controls. Gene expression profiles were measured in leaf samples with the 20 K NSF oligonucleotide microarray. A linear model was fitted to the data to identify genes that were significantly regulated under drought stress. In all drought stressed cultivars, 245 genes were significantly repressed and 413 genes induced. Genes differing in their expression pattern under drought stress between tolerant and sensitive cultivars were identified by the genotype × environment (G × E) interaction term. More genes were significantly drought regulated in the sensitive than in the tolerant cultivars. Localizing all expressed genes on the rice genome map, we checked which genes with a significant G × E interaction co-localized with published quantitative trait loci regions for drought tolerance. These genes are more likely to be important for drought tolerance in an agricultural environment. To identify the metabolic processes with a significant G × E effect, we adapted the analysis software MapMan for rice. We found a drought stress induced shift toward senescence related degradation processes that was more pronounced in the sensitive than in the tolerant cultivars. In spite of higher growth rates and water use, more photosynthesis related genes were down-regulated in the tolerant than in the sensitive cultivars. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11103-008-9412-7) contains supplementary material, which is available to authorized users. Springer Netherlands 2008-10-19 2009-01 /pmc/articles/PMC2709230/ /pubmed/18931976 http://dx.doi.org/10.1007/s11103-008-9412-7 Text en © The Author(s) 2008 |
spellingShingle | Article Degenkolbe, Thomas Do, Phuc Thi Zuther, Ellen Repsilber, Dirk Walther, Dirk Hincha, Dirk K. Köhl, Karin I. Expression profiling of rice cultivars differing in their tolerance to long-term drought stress |
title | Expression profiling of rice cultivars differing in their tolerance to long-term drought stress |
title_full | Expression profiling of rice cultivars differing in their tolerance to long-term drought stress |
title_fullStr | Expression profiling of rice cultivars differing in their tolerance to long-term drought stress |
title_full_unstemmed | Expression profiling of rice cultivars differing in their tolerance to long-term drought stress |
title_short | Expression profiling of rice cultivars differing in their tolerance to long-term drought stress |
title_sort | expression profiling of rice cultivars differing in their tolerance to long-term drought stress |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2709230/ https://www.ncbi.nlm.nih.gov/pubmed/18931976 http://dx.doi.org/10.1007/s11103-008-9412-7 |
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