Coordinated changes in energy intake and expenditure following hypothalamic administration of neuropeptides involved in energy balance

OBJECTIVE: The hypothalamic control of energy balance is regulated by a complex network of neuropeptide-releasing neurons. Whilst the effect of these neuropeptides on individual aspects of energy homeostasis has been studied, the coordinated response of these effects has not been comprehensively inv...

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Autores principales: Semjonous, Nina M, Smith, Kirsty L, Parkinson, James RC, Gunner, David JL, Liu, Yong-Ling, Murphy, Kevin G, Ghatei, Mohammad A, Bloom, Stephen R, Small, Caroline J
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2711051/
https://www.ncbi.nlm.nih.gov/pubmed/19488048
http://dx.doi.org/10.1038/ijo.2009.96
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author Semjonous, Nina M
Smith, Kirsty L
Parkinson, James RC
Gunner, David JL
Liu, Yong-Ling
Murphy, Kevin G
Ghatei, Mohammad A
Bloom, Stephen R
Small, Caroline J
author_facet Semjonous, Nina M
Smith, Kirsty L
Parkinson, James RC
Gunner, David JL
Liu, Yong-Ling
Murphy, Kevin G
Ghatei, Mohammad A
Bloom, Stephen R
Small, Caroline J
author_sort Semjonous, Nina M
collection PubMed
description OBJECTIVE: The hypothalamic control of energy balance is regulated by a complex network of neuropeptide-releasing neurons. Whilst the effect of these neuropeptides on individual aspects of energy homeostasis has been studied, the coordinated response of these effects has not been comprehensively investigated. We have simultaneously monitored a number of metabolic parameters following ICV administration of 1nmol and 3nmol of neuropeptides with established roles in the regulation of feeding, activity and metabolism. Ad libitum fed rats received the orexigenic neuropeptides neuropeptide Y (NPY), agouti-related protein (AgRP), melanin-concentrating hormone (MCH) or orexin-A. Overnight food deprived rats received an ICV injection of the anorectic peptides α-MSH, corticotrophin releasing factor (CRF) or neuromedin U (NMU). RESULTS: Our results reveal the temporal sequence of the effects of these neuropeptides on both energy intake and expenditure, highlighting key differences in their function as mediators of energy balance. NPY and AgRP increased feeding and decreased oxygen consumption, with the effects of AgRP being more prolonged. In contrast, orexin-A increased both feeding and oxygen consumption, consistent with an observed increase in activity. The potent anorexigenic effects of CRF were accompanied by a prolonged increase in activity whilst NMU injection resulted in significant but short-lasting inhibition of food intake, ambulatory activity and oxygen consumption. Alpha-MSH injection resulted in significant increases in both ambulatory activity and oxygen consumption, and reduced food intake following administration of 3nmol of the peptide. CONCLUSION: We have for the first time, simultaneously measured several metabolic parameters following hypothalamic administration of a number of neuropeptides within the same experimental system. This work has demonstrated the interrelated effects of these neuropeotides on activity, energy expenditure and food intake thus facilitating comparison between the different hypothalamic systems.
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spelling pubmed-27110512010-01-01 Coordinated changes in energy intake and expenditure following hypothalamic administration of neuropeptides involved in energy balance Semjonous, Nina M Smith, Kirsty L Parkinson, James RC Gunner, David JL Liu, Yong-Ling Murphy, Kevin G Ghatei, Mohammad A Bloom, Stephen R Small, Caroline J Int J Obes (Lond) Article OBJECTIVE: The hypothalamic control of energy balance is regulated by a complex network of neuropeptide-releasing neurons. Whilst the effect of these neuropeptides on individual aspects of energy homeostasis has been studied, the coordinated response of these effects has not been comprehensively investigated. We have simultaneously monitored a number of metabolic parameters following ICV administration of 1nmol and 3nmol of neuropeptides with established roles in the regulation of feeding, activity and metabolism. Ad libitum fed rats received the orexigenic neuropeptides neuropeptide Y (NPY), agouti-related protein (AgRP), melanin-concentrating hormone (MCH) or orexin-A. Overnight food deprived rats received an ICV injection of the anorectic peptides α-MSH, corticotrophin releasing factor (CRF) or neuromedin U (NMU). RESULTS: Our results reveal the temporal sequence of the effects of these neuropeptides on both energy intake and expenditure, highlighting key differences in their function as mediators of energy balance. NPY and AgRP increased feeding and decreased oxygen consumption, with the effects of AgRP being more prolonged. In contrast, orexin-A increased both feeding and oxygen consumption, consistent with an observed increase in activity. The potent anorexigenic effects of CRF were accompanied by a prolonged increase in activity whilst NMU injection resulted in significant but short-lasting inhibition of food intake, ambulatory activity and oxygen consumption. Alpha-MSH injection resulted in significant increases in both ambulatory activity and oxygen consumption, and reduced food intake following administration of 3nmol of the peptide. CONCLUSION: We have for the first time, simultaneously measured several metabolic parameters following hypothalamic administration of a number of neuropeptides within the same experimental system. This work has demonstrated the interrelated effects of these neuropeotides on activity, energy expenditure and food intake thus facilitating comparison between the different hypothalamic systems. 2009-06-02 2009-07 /pmc/articles/PMC2711051/ /pubmed/19488048 http://dx.doi.org/10.1038/ijo.2009.96 Text en
spellingShingle Article
Semjonous, Nina M
Smith, Kirsty L
Parkinson, James RC
Gunner, David JL
Liu, Yong-Ling
Murphy, Kevin G
Ghatei, Mohammad A
Bloom, Stephen R
Small, Caroline J
Coordinated changes in energy intake and expenditure following hypothalamic administration of neuropeptides involved in energy balance
title Coordinated changes in energy intake and expenditure following hypothalamic administration of neuropeptides involved in energy balance
title_full Coordinated changes in energy intake and expenditure following hypothalamic administration of neuropeptides involved in energy balance
title_fullStr Coordinated changes in energy intake and expenditure following hypothalamic administration of neuropeptides involved in energy balance
title_full_unstemmed Coordinated changes in energy intake and expenditure following hypothalamic administration of neuropeptides involved in energy balance
title_short Coordinated changes in energy intake and expenditure following hypothalamic administration of neuropeptides involved in energy balance
title_sort coordinated changes in energy intake and expenditure following hypothalamic administration of neuropeptides involved in energy balance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2711051/
https://www.ncbi.nlm.nih.gov/pubmed/19488048
http://dx.doi.org/10.1038/ijo.2009.96
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