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Classical cadherins control nucleus and centrosome position and cell polarity

Control of cell polarity is crucial during tissue morphogenesis and renewal, and depends on spatial cues provided by the extracellular environment. Using micropatterned substrates to impose reproducible cell–cell interactions, we show that in the absence of other polarizing cues, cell–cell contacts...

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Detalles Bibliográficos
Autores principales: Dupin, Isabelle, Camand, Emeline, Etienne-Manneville, Sandrine
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2711586/
https://www.ncbi.nlm.nih.gov/pubmed/19487453
http://dx.doi.org/10.1083/jcb.200812034
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author Dupin, Isabelle
Camand, Emeline
Etienne-Manneville, Sandrine
author_facet Dupin, Isabelle
Camand, Emeline
Etienne-Manneville, Sandrine
author_sort Dupin, Isabelle
collection PubMed
description Control of cell polarity is crucial during tissue morphogenesis and renewal, and depends on spatial cues provided by the extracellular environment. Using micropatterned substrates to impose reproducible cell–cell interactions, we show that in the absence of other polarizing cues, cell–cell contacts are the main regulator of nucleus and centrosome positioning, and intracellular polarized organization. In a variety of cell types, including astrocytes, epithelial cells, and endothelial cells, calcium-dependent cadherin-mediated cell–cell interactions induce nucleus and centrosome off-centering toward cell–cell contacts, and promote orientation of the nucleus–centrosome axis toward free cell edges. Nucleus and centrosome off-centering is controlled by N-cadherin through the regulation of cell interactions with the extracellular matrix, whereas the orientation of the nucleus–centrosome axis is determined by the geometry of N-cadherin–mediated contacts. Our results demonstrate that in addition to the specific function of E-cadherin in regulating baso-apical epithelial polarity, classical cadherins control cell polarization in otherwise nonpolarized cells.
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spelling pubmed-27115862009-12-01 Classical cadherins control nucleus and centrosome position and cell polarity Dupin, Isabelle Camand, Emeline Etienne-Manneville, Sandrine J Cell Biol Research Articles Control of cell polarity is crucial during tissue morphogenesis and renewal, and depends on spatial cues provided by the extracellular environment. Using micropatterned substrates to impose reproducible cell–cell interactions, we show that in the absence of other polarizing cues, cell–cell contacts are the main regulator of nucleus and centrosome positioning, and intracellular polarized organization. In a variety of cell types, including astrocytes, epithelial cells, and endothelial cells, calcium-dependent cadherin-mediated cell–cell interactions induce nucleus and centrosome off-centering toward cell–cell contacts, and promote orientation of the nucleus–centrosome axis toward free cell edges. Nucleus and centrosome off-centering is controlled by N-cadherin through the regulation of cell interactions with the extracellular matrix, whereas the orientation of the nucleus–centrosome axis is determined by the geometry of N-cadherin–mediated contacts. Our results demonstrate that in addition to the specific function of E-cadherin in regulating baso-apical epithelial polarity, classical cadherins control cell polarization in otherwise nonpolarized cells. The Rockefeller University Press 2009-06-01 /pmc/articles/PMC2711586/ /pubmed/19487453 http://dx.doi.org/10.1083/jcb.200812034 Text en © 2009 Dupin et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Dupin, Isabelle
Camand, Emeline
Etienne-Manneville, Sandrine
Classical cadherins control nucleus and centrosome position and cell polarity
title Classical cadherins control nucleus and centrosome position and cell polarity
title_full Classical cadherins control nucleus and centrosome position and cell polarity
title_fullStr Classical cadherins control nucleus and centrosome position and cell polarity
title_full_unstemmed Classical cadherins control nucleus and centrosome position and cell polarity
title_short Classical cadherins control nucleus and centrosome position and cell polarity
title_sort classical cadherins control nucleus and centrosome position and cell polarity
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2711586/
https://www.ncbi.nlm.nih.gov/pubmed/19487453
http://dx.doi.org/10.1083/jcb.200812034
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