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Classical cadherins control nucleus and centrosome position and cell polarity
Control of cell polarity is crucial during tissue morphogenesis and renewal, and depends on spatial cues provided by the extracellular environment. Using micropatterned substrates to impose reproducible cell–cell interactions, we show that in the absence of other polarizing cues, cell–cell contacts...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2711586/ https://www.ncbi.nlm.nih.gov/pubmed/19487453 http://dx.doi.org/10.1083/jcb.200812034 |
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author | Dupin, Isabelle Camand, Emeline Etienne-Manneville, Sandrine |
author_facet | Dupin, Isabelle Camand, Emeline Etienne-Manneville, Sandrine |
author_sort | Dupin, Isabelle |
collection | PubMed |
description | Control of cell polarity is crucial during tissue morphogenesis and renewal, and depends on spatial cues provided by the extracellular environment. Using micropatterned substrates to impose reproducible cell–cell interactions, we show that in the absence of other polarizing cues, cell–cell contacts are the main regulator of nucleus and centrosome positioning, and intracellular polarized organization. In a variety of cell types, including astrocytes, epithelial cells, and endothelial cells, calcium-dependent cadherin-mediated cell–cell interactions induce nucleus and centrosome off-centering toward cell–cell contacts, and promote orientation of the nucleus–centrosome axis toward free cell edges. Nucleus and centrosome off-centering is controlled by N-cadherin through the regulation of cell interactions with the extracellular matrix, whereas the orientation of the nucleus–centrosome axis is determined by the geometry of N-cadherin–mediated contacts. Our results demonstrate that in addition to the specific function of E-cadherin in regulating baso-apical epithelial polarity, classical cadherins control cell polarization in otherwise nonpolarized cells. |
format | Text |
id | pubmed-2711586 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-27115862009-12-01 Classical cadherins control nucleus and centrosome position and cell polarity Dupin, Isabelle Camand, Emeline Etienne-Manneville, Sandrine J Cell Biol Research Articles Control of cell polarity is crucial during tissue morphogenesis and renewal, and depends on spatial cues provided by the extracellular environment. Using micropatterned substrates to impose reproducible cell–cell interactions, we show that in the absence of other polarizing cues, cell–cell contacts are the main regulator of nucleus and centrosome positioning, and intracellular polarized organization. In a variety of cell types, including astrocytes, epithelial cells, and endothelial cells, calcium-dependent cadherin-mediated cell–cell interactions induce nucleus and centrosome off-centering toward cell–cell contacts, and promote orientation of the nucleus–centrosome axis toward free cell edges. Nucleus and centrosome off-centering is controlled by N-cadherin through the regulation of cell interactions with the extracellular matrix, whereas the orientation of the nucleus–centrosome axis is determined by the geometry of N-cadherin–mediated contacts. Our results demonstrate that in addition to the specific function of E-cadherin in regulating baso-apical epithelial polarity, classical cadherins control cell polarization in otherwise nonpolarized cells. The Rockefeller University Press 2009-06-01 /pmc/articles/PMC2711586/ /pubmed/19487453 http://dx.doi.org/10.1083/jcb.200812034 Text en © 2009 Dupin et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Dupin, Isabelle Camand, Emeline Etienne-Manneville, Sandrine Classical cadherins control nucleus and centrosome position and cell polarity |
title | Classical cadherins control nucleus and centrosome position and cell polarity |
title_full | Classical cadherins control nucleus and centrosome position and cell polarity |
title_fullStr | Classical cadherins control nucleus and centrosome position and cell polarity |
title_full_unstemmed | Classical cadherins control nucleus and centrosome position and cell polarity |
title_short | Classical cadherins control nucleus and centrosome position and cell polarity |
title_sort | classical cadherins control nucleus and centrosome position and cell polarity |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2711586/ https://www.ncbi.nlm.nih.gov/pubmed/19487453 http://dx.doi.org/10.1083/jcb.200812034 |
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