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Mammalian Fat and Dachsous cadherins regulate apical membrane organization in the embryonic cerebral cortex

Compartmentalization of the plasma membrane in a cell is fundamental for its proper functions. In this study, we present evidence that mammalian Fat4 and Dachsous1 cadherins regulate the apical plasma membrane organization in the embryonic cerebral cortex. In neural progenitor cells of the cortex, F...

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Autores principales: Ishiuchi, Takashi, Misaki, Kazuyo, Yonemura, Shigenobu, Takeichi, Masatoshi, Tanoue, Takuji
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2711618/
https://www.ncbi.nlm.nih.gov/pubmed/19506035
http://dx.doi.org/10.1083/jcb.200811030
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author Ishiuchi, Takashi
Misaki, Kazuyo
Yonemura, Shigenobu
Takeichi, Masatoshi
Tanoue, Takuji
author_facet Ishiuchi, Takashi
Misaki, Kazuyo
Yonemura, Shigenobu
Takeichi, Masatoshi
Tanoue, Takuji
author_sort Ishiuchi, Takashi
collection PubMed
description Compartmentalization of the plasma membrane in a cell is fundamental for its proper functions. In this study, we present evidence that mammalian Fat4 and Dachsous1 cadherins regulate the apical plasma membrane organization in the embryonic cerebral cortex. In neural progenitor cells of the cortex, Fat4 and Dachsous1 were concentrated together in a cell–cell contact area positioned more apically than the adherens junction (AJ). These molecules interacted in a heterophilic fashion, affecting their respective protein levels. We further found that Fat4 associated and colocalized with the Pals1 complex. Ultrastructurally, the apical junctions of the progenitor cells comprised the AJ and a stretch of plasma membrane apposition extending apically from the AJ, which positionally corresponded to the Fat4–Dachsous1-positive zone. Depletion of Fat4 or Pals1 abolished this membrane apposition. These results highlight the importance of the Fat4–Dachsous1–Pals1 complex in organizing the apical membrane architecture of neural progenitor cells.
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spelling pubmed-27116182009-12-15 Mammalian Fat and Dachsous cadherins regulate apical membrane organization in the embryonic cerebral cortex Ishiuchi, Takashi Misaki, Kazuyo Yonemura, Shigenobu Takeichi, Masatoshi Tanoue, Takuji J Cell Biol Research Articles Compartmentalization of the plasma membrane in a cell is fundamental for its proper functions. In this study, we present evidence that mammalian Fat4 and Dachsous1 cadherins regulate the apical plasma membrane organization in the embryonic cerebral cortex. In neural progenitor cells of the cortex, Fat4 and Dachsous1 were concentrated together in a cell–cell contact area positioned more apically than the adherens junction (AJ). These molecules interacted in a heterophilic fashion, affecting their respective protein levels. We further found that Fat4 associated and colocalized with the Pals1 complex. Ultrastructurally, the apical junctions of the progenitor cells comprised the AJ and a stretch of plasma membrane apposition extending apically from the AJ, which positionally corresponded to the Fat4–Dachsous1-positive zone. Depletion of Fat4 or Pals1 abolished this membrane apposition. These results highlight the importance of the Fat4–Dachsous1–Pals1 complex in organizing the apical membrane architecture of neural progenitor cells. The Rockefeller University Press 2009-06-15 /pmc/articles/PMC2711618/ /pubmed/19506035 http://dx.doi.org/10.1083/jcb.200811030 Text en © 2009 Ishiuchi et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Ishiuchi, Takashi
Misaki, Kazuyo
Yonemura, Shigenobu
Takeichi, Masatoshi
Tanoue, Takuji
Mammalian Fat and Dachsous cadherins regulate apical membrane organization in the embryonic cerebral cortex
title Mammalian Fat and Dachsous cadherins regulate apical membrane organization in the embryonic cerebral cortex
title_full Mammalian Fat and Dachsous cadherins regulate apical membrane organization in the embryonic cerebral cortex
title_fullStr Mammalian Fat and Dachsous cadherins regulate apical membrane organization in the embryonic cerebral cortex
title_full_unstemmed Mammalian Fat and Dachsous cadherins regulate apical membrane organization in the embryonic cerebral cortex
title_short Mammalian Fat and Dachsous cadherins regulate apical membrane organization in the embryonic cerebral cortex
title_sort mammalian fat and dachsous cadherins regulate apical membrane organization in the embryonic cerebral cortex
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2711618/
https://www.ncbi.nlm.nih.gov/pubmed/19506035
http://dx.doi.org/10.1083/jcb.200811030
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