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Mammalian Fat and Dachsous cadherins regulate apical membrane organization in the embryonic cerebral cortex
Compartmentalization of the plasma membrane in a cell is fundamental for its proper functions. In this study, we present evidence that mammalian Fat4 and Dachsous1 cadherins regulate the apical plasma membrane organization in the embryonic cerebral cortex. In neural progenitor cells of the cortex, F...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2711618/ https://www.ncbi.nlm.nih.gov/pubmed/19506035 http://dx.doi.org/10.1083/jcb.200811030 |
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author | Ishiuchi, Takashi Misaki, Kazuyo Yonemura, Shigenobu Takeichi, Masatoshi Tanoue, Takuji |
author_facet | Ishiuchi, Takashi Misaki, Kazuyo Yonemura, Shigenobu Takeichi, Masatoshi Tanoue, Takuji |
author_sort | Ishiuchi, Takashi |
collection | PubMed |
description | Compartmentalization of the plasma membrane in a cell is fundamental for its proper functions. In this study, we present evidence that mammalian Fat4 and Dachsous1 cadherins regulate the apical plasma membrane organization in the embryonic cerebral cortex. In neural progenitor cells of the cortex, Fat4 and Dachsous1 were concentrated together in a cell–cell contact area positioned more apically than the adherens junction (AJ). These molecules interacted in a heterophilic fashion, affecting their respective protein levels. We further found that Fat4 associated and colocalized with the Pals1 complex. Ultrastructurally, the apical junctions of the progenitor cells comprised the AJ and a stretch of plasma membrane apposition extending apically from the AJ, which positionally corresponded to the Fat4–Dachsous1-positive zone. Depletion of Fat4 or Pals1 abolished this membrane apposition. These results highlight the importance of the Fat4–Dachsous1–Pals1 complex in organizing the apical membrane architecture of neural progenitor cells. |
format | Text |
id | pubmed-2711618 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-27116182009-12-15 Mammalian Fat and Dachsous cadherins regulate apical membrane organization in the embryonic cerebral cortex Ishiuchi, Takashi Misaki, Kazuyo Yonemura, Shigenobu Takeichi, Masatoshi Tanoue, Takuji J Cell Biol Research Articles Compartmentalization of the plasma membrane in a cell is fundamental for its proper functions. In this study, we present evidence that mammalian Fat4 and Dachsous1 cadherins regulate the apical plasma membrane organization in the embryonic cerebral cortex. In neural progenitor cells of the cortex, Fat4 and Dachsous1 were concentrated together in a cell–cell contact area positioned more apically than the adherens junction (AJ). These molecules interacted in a heterophilic fashion, affecting their respective protein levels. We further found that Fat4 associated and colocalized with the Pals1 complex. Ultrastructurally, the apical junctions of the progenitor cells comprised the AJ and a stretch of plasma membrane apposition extending apically from the AJ, which positionally corresponded to the Fat4–Dachsous1-positive zone. Depletion of Fat4 or Pals1 abolished this membrane apposition. These results highlight the importance of the Fat4–Dachsous1–Pals1 complex in organizing the apical membrane architecture of neural progenitor cells. The Rockefeller University Press 2009-06-15 /pmc/articles/PMC2711618/ /pubmed/19506035 http://dx.doi.org/10.1083/jcb.200811030 Text en © 2009 Ishiuchi et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Ishiuchi, Takashi Misaki, Kazuyo Yonemura, Shigenobu Takeichi, Masatoshi Tanoue, Takuji Mammalian Fat and Dachsous cadherins regulate apical membrane organization in the embryonic cerebral cortex |
title | Mammalian Fat and Dachsous cadherins regulate apical membrane organization in the embryonic cerebral cortex |
title_full | Mammalian Fat and Dachsous cadherins regulate apical membrane organization in the embryonic cerebral cortex |
title_fullStr | Mammalian Fat and Dachsous cadherins regulate apical membrane organization in the embryonic cerebral cortex |
title_full_unstemmed | Mammalian Fat and Dachsous cadherins regulate apical membrane organization in the embryonic cerebral cortex |
title_short | Mammalian Fat and Dachsous cadherins regulate apical membrane organization in the embryonic cerebral cortex |
title_sort | mammalian fat and dachsous cadherins regulate apical membrane organization in the embryonic cerebral cortex |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2711618/ https://www.ncbi.nlm.nih.gov/pubmed/19506035 http://dx.doi.org/10.1083/jcb.200811030 |
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