Cargando…
Evidence of Recombination and Genetic Diversity in Human Rhinoviruses in Children with Acute Respiratory Infection
BACKGROUND: Human rhinoviruses (HRVs) are a highly prevalent cause of acute respiratory infection in children. They are classified into at least three species, HRV-A, HRV-B and HRV-C, which are characterized by sequencing the 5′ untranslated region (UTR) or the VP4/VP2 region of the genome. Given th...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2712091/ https://www.ncbi.nlm.nih.gov/pubmed/19633719 http://dx.doi.org/10.1371/journal.pone.0006355 |
_version_ | 1782169478586433536 |
---|---|
author | Huang, Ting Wang, Wei Bessaud, Mael Ren, Peijun Sheng, Jun Yan, Huajie Zhang, Jing Lin, Xin Wang, Yongjin Delpeyroux, Francis Deubel, Vincent |
author_facet | Huang, Ting Wang, Wei Bessaud, Mael Ren, Peijun Sheng, Jun Yan, Huajie Zhang, Jing Lin, Xin Wang, Yongjin Delpeyroux, Francis Deubel, Vincent |
author_sort | Huang, Ting |
collection | PubMed |
description | BACKGROUND: Human rhinoviruses (HRVs) are a highly prevalent cause of acute respiratory infection in children. They are classified into at least three species, HRV-A, HRV-B and HRV-C, which are characterized by sequencing the 5′ untranslated region (UTR) or the VP4/VP2 region of the genome. Given the increased interest for novel HRV strain identification and their worldwide distribution, we have carried out clinical and molecular diagnosis of HRV strains in a 2-year study of children with acute respiratory infection visiting one district hospital in Shanghai. METHODOLOGY/FINDINGS: We cloned and sequenced a 924-nt fragment that covered part of the 5′UTR and the VP4/VP2 capsid genes. Sixty-four HRV-infected outpatients were diagnosed amongst 827 children with acute low respiratory tract infection. Two samples were co-infected with HRV-A and HRV-B or HRV-C. By comparative analysis of the VP4/VP2 sequences of the 66 HRVs, we showed a high diversity of strains in HRV-A and HRV-B species, and a prevalence of 51.5% of strains that belonged to the recently identified HRV-C species. When analyzing a fragment of the 5′ UTR, we characterized at least two subspecies of HRV-C: HRV-Cc, which clustered differently from HRV-A and HRV-B, and HRV-Ca, which resulted from previous recombination in this region with sequences related to HRV-A. The full-length sequence of one strain of each HRV-Ca and HRV-Cc subspecies was obtained for comparative analysis. We confirmed the close relationship of their structural proteins but showed apparent additional recombination events in the 2A gene and 3′UTR of the HRV-Ca strain. Double or triple infections with HRV-C and respiratory syncytial virus and/or bocavirus were diagnosed in 33.3% of the HRV-infected patients, but no correlation with severity of clinical outcome was observed. CONCLUSION: Our study showed a high diversity of HRV strains that cause bronchitis and pneumonia in children. A predominance of HRV-C over HRV-A and HRV-B was observed, and two subspecies of HRV-C were identified, the diversity of which seemed to be related to recombination with former HRV-A strains. None of the HRV-C strains appeared to have a higher clinical impact than HRV-A or HRV-B on respiratory compromise. |
format | Text |
id | pubmed-2712091 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-27120912009-07-27 Evidence of Recombination and Genetic Diversity in Human Rhinoviruses in Children with Acute Respiratory Infection Huang, Ting Wang, Wei Bessaud, Mael Ren, Peijun Sheng, Jun Yan, Huajie Zhang, Jing Lin, Xin Wang, Yongjin Delpeyroux, Francis Deubel, Vincent PLoS One Research Article BACKGROUND: Human rhinoviruses (HRVs) are a highly prevalent cause of acute respiratory infection in children. They are classified into at least three species, HRV-A, HRV-B and HRV-C, which are characterized by sequencing the 5′ untranslated region (UTR) or the VP4/VP2 region of the genome. Given the increased interest for novel HRV strain identification and their worldwide distribution, we have carried out clinical and molecular diagnosis of HRV strains in a 2-year study of children with acute respiratory infection visiting one district hospital in Shanghai. METHODOLOGY/FINDINGS: We cloned and sequenced a 924-nt fragment that covered part of the 5′UTR and the VP4/VP2 capsid genes. Sixty-four HRV-infected outpatients were diagnosed amongst 827 children with acute low respiratory tract infection. Two samples were co-infected with HRV-A and HRV-B or HRV-C. By comparative analysis of the VP4/VP2 sequences of the 66 HRVs, we showed a high diversity of strains in HRV-A and HRV-B species, and a prevalence of 51.5% of strains that belonged to the recently identified HRV-C species. When analyzing a fragment of the 5′ UTR, we characterized at least two subspecies of HRV-C: HRV-Cc, which clustered differently from HRV-A and HRV-B, and HRV-Ca, which resulted from previous recombination in this region with sequences related to HRV-A. The full-length sequence of one strain of each HRV-Ca and HRV-Cc subspecies was obtained for comparative analysis. We confirmed the close relationship of their structural proteins but showed apparent additional recombination events in the 2A gene and 3′UTR of the HRV-Ca strain. Double or triple infections with HRV-C and respiratory syncytial virus and/or bocavirus were diagnosed in 33.3% of the HRV-infected patients, but no correlation with severity of clinical outcome was observed. CONCLUSION: Our study showed a high diversity of HRV strains that cause bronchitis and pneumonia in children. A predominance of HRV-C over HRV-A and HRV-B was observed, and two subspecies of HRV-C were identified, the diversity of which seemed to be related to recombination with former HRV-A strains. None of the HRV-C strains appeared to have a higher clinical impact than HRV-A or HRV-B on respiratory compromise. Public Library of Science 2009-07-27 /pmc/articles/PMC2712091/ /pubmed/19633719 http://dx.doi.org/10.1371/journal.pone.0006355 Text en Huang et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Huang, Ting Wang, Wei Bessaud, Mael Ren, Peijun Sheng, Jun Yan, Huajie Zhang, Jing Lin, Xin Wang, Yongjin Delpeyroux, Francis Deubel, Vincent Evidence of Recombination and Genetic Diversity in Human Rhinoviruses in Children with Acute Respiratory Infection |
title | Evidence of Recombination and Genetic Diversity in Human Rhinoviruses in Children with Acute Respiratory Infection |
title_full | Evidence of Recombination and Genetic Diversity in Human Rhinoviruses in Children with Acute Respiratory Infection |
title_fullStr | Evidence of Recombination and Genetic Diversity in Human Rhinoviruses in Children with Acute Respiratory Infection |
title_full_unstemmed | Evidence of Recombination and Genetic Diversity in Human Rhinoviruses in Children with Acute Respiratory Infection |
title_short | Evidence of Recombination and Genetic Diversity in Human Rhinoviruses in Children with Acute Respiratory Infection |
title_sort | evidence of recombination and genetic diversity in human rhinoviruses in children with acute respiratory infection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2712091/ https://www.ncbi.nlm.nih.gov/pubmed/19633719 http://dx.doi.org/10.1371/journal.pone.0006355 |
work_keys_str_mv | AT huangting evidenceofrecombinationandgeneticdiversityinhumanrhinovirusesinchildrenwithacuterespiratoryinfection AT wangwei evidenceofrecombinationandgeneticdiversityinhumanrhinovirusesinchildrenwithacuterespiratoryinfection AT bessaudmael evidenceofrecombinationandgeneticdiversityinhumanrhinovirusesinchildrenwithacuterespiratoryinfection AT renpeijun evidenceofrecombinationandgeneticdiversityinhumanrhinovirusesinchildrenwithacuterespiratoryinfection AT shengjun evidenceofrecombinationandgeneticdiversityinhumanrhinovirusesinchildrenwithacuterespiratoryinfection AT yanhuajie evidenceofrecombinationandgeneticdiversityinhumanrhinovirusesinchildrenwithacuterespiratoryinfection AT zhangjing evidenceofrecombinationandgeneticdiversityinhumanrhinovirusesinchildrenwithacuterespiratoryinfection AT linxin evidenceofrecombinationandgeneticdiversityinhumanrhinovirusesinchildrenwithacuterespiratoryinfection AT wangyongjin evidenceofrecombinationandgeneticdiversityinhumanrhinovirusesinchildrenwithacuterespiratoryinfection AT delpeyrouxfrancis evidenceofrecombinationandgeneticdiversityinhumanrhinovirusesinchildrenwithacuterespiratoryinfection AT deubelvincent evidenceofrecombinationandgeneticdiversityinhumanrhinovirusesinchildrenwithacuterespiratoryinfection |