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Ca(2+) signaling evoked by activation of Na(+) channels and Na(+)/Ca(2+) exchangers is required for GABA-induced NG2 cell migration

NG2 cells originate from various brain regions and migrate to their destinations during early development. These cells express voltage-gated Na(+) channels but fail to produce typical action potentials. The physiological role of Na(+) channels in these cells is unclear. We found that GABA induces me...

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Autores principales: Tong, Xiao-ping, Li, Xiang-yao, Zhou, Bing, Shen, Wanhua, Zhang, Zhi-jun, Xu, Tian-le, Duan, Shumin
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2712990/
https://www.ncbi.nlm.nih.gov/pubmed/19596850
http://dx.doi.org/10.1083/jcb.200811071
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author Tong, Xiao-ping
Li, Xiang-yao
Zhou, Bing
Shen, Wanhua
Zhang, Zhi-jun
Xu, Tian-le
Duan, Shumin
author_facet Tong, Xiao-ping
Li, Xiang-yao
Zhou, Bing
Shen, Wanhua
Zhang, Zhi-jun
Xu, Tian-le
Duan, Shumin
author_sort Tong, Xiao-ping
collection PubMed
description NG2 cells originate from various brain regions and migrate to their destinations during early development. These cells express voltage-gated Na(+) channels but fail to produce typical action potentials. The physiological role of Na(+) channels in these cells is unclear. We found that GABA induces membrane depolarization and Ca(2+) elevation in NG2 cells, a process requiring activation of GABA(A) receptors, Na(+) channels, and Na(+)/Ca(2+) exchangers (NCXs), but not Ca(2+) channels. We have identified a persistent Na(+) current in these cells that may underlie the GABA-induced pathway of prolonged Na(+) elevation, which in turn triggers Ca(2+) influx via NCXs. This unique Ca(2+) signaling pathway is further shown to be involved in the migration of NG2 cells. Thus, GABAergic signaling mediated by sequential activation of GABA(A) receptors, noninactivating Na(+) channels, and NCXs may play an important role in the development and function of NG2 glial cells in the brain.
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spelling pubmed-27129902010-01-13 Ca(2+) signaling evoked by activation of Na(+) channels and Na(+)/Ca(2+) exchangers is required for GABA-induced NG2 cell migration Tong, Xiao-ping Li, Xiang-yao Zhou, Bing Shen, Wanhua Zhang, Zhi-jun Xu, Tian-le Duan, Shumin J Cell Biol Research Articles NG2 cells originate from various brain regions and migrate to their destinations during early development. These cells express voltage-gated Na(+) channels but fail to produce typical action potentials. The physiological role of Na(+) channels in these cells is unclear. We found that GABA induces membrane depolarization and Ca(2+) elevation in NG2 cells, a process requiring activation of GABA(A) receptors, Na(+) channels, and Na(+)/Ca(2+) exchangers (NCXs), but not Ca(2+) channels. We have identified a persistent Na(+) current in these cells that may underlie the GABA-induced pathway of prolonged Na(+) elevation, which in turn triggers Ca(2+) influx via NCXs. This unique Ca(2+) signaling pathway is further shown to be involved in the migration of NG2 cells. Thus, GABAergic signaling mediated by sequential activation of GABA(A) receptors, noninactivating Na(+) channels, and NCXs may play an important role in the development and function of NG2 glial cells in the brain. The Rockefeller University Press 2009-07-13 /pmc/articles/PMC2712990/ /pubmed/19596850 http://dx.doi.org/10.1083/jcb.200811071 Text en © 2009 Tong et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Tong, Xiao-ping
Li, Xiang-yao
Zhou, Bing
Shen, Wanhua
Zhang, Zhi-jun
Xu, Tian-le
Duan, Shumin
Ca(2+) signaling evoked by activation of Na(+) channels and Na(+)/Ca(2+) exchangers is required for GABA-induced NG2 cell migration
title Ca(2+) signaling evoked by activation of Na(+) channels and Na(+)/Ca(2+) exchangers is required for GABA-induced NG2 cell migration
title_full Ca(2+) signaling evoked by activation of Na(+) channels and Na(+)/Ca(2+) exchangers is required for GABA-induced NG2 cell migration
title_fullStr Ca(2+) signaling evoked by activation of Na(+) channels and Na(+)/Ca(2+) exchangers is required for GABA-induced NG2 cell migration
title_full_unstemmed Ca(2+) signaling evoked by activation of Na(+) channels and Na(+)/Ca(2+) exchangers is required for GABA-induced NG2 cell migration
title_short Ca(2+) signaling evoked by activation of Na(+) channels and Na(+)/Ca(2+) exchangers is required for GABA-induced NG2 cell migration
title_sort ca(2+) signaling evoked by activation of na(+) channels and na(+)/ca(2+) exchangers is required for gaba-induced ng2 cell migration
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2712990/
https://www.ncbi.nlm.nih.gov/pubmed/19596850
http://dx.doi.org/10.1083/jcb.200811071
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