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Efficient targeted transcript discovery via array-based normalization of RACE libraries

RACE (Rapid Amplification of cDNA Ends) is a widely used approach for transcript identification. Random clone selection from the RACE mixture, however, is an ineffective sampling strategy if the dynamic range of transcript abundances is large. Here, we describe a strategy that uses array hybridizati...

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Detalles Bibliográficos
Autores principales: Djebali, Sarah, Kapranov, Philipp, Foissac, Sylvain, Lagarde, Julien, Reymond, Alexandre, Ucla, Catherine, Wyss, Carine, Drenkow, Jorg, Dumais, Erica, Murray, Ryan R., Lin, Chenwei, Szeto, David, Denoeud, France, Calvo, Miquel, Frankish, Adam, Harrow, Jennifer, Makrythanasis, Periklis, Vidal, Marc, Salehi-Ashtiani, Kourosh, Antonarakis, Stylianos E., Gingeras, Thomas R., Guigó, Roderic
Formato: Texto
Lenguaje:English
Publicado: 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2713501/
https://www.ncbi.nlm.nih.gov/pubmed/18500348
http://dx.doi.org/10.1038/nmeth.1216

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