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Efficient targeted transcript discovery via array-based normalization of RACE libraries
RACE (Rapid Amplification of cDNA Ends) is a widely used approach for transcript identification. Random clone selection from the RACE mixture, however, is an ineffective sampling strategy if the dynamic range of transcript abundances is large. Here, we describe a strategy that uses array hybridizati...
Autores principales: | Djebali, Sarah, Kapranov, Philipp, Foissac, Sylvain, Lagarde, Julien, Reymond, Alexandre, Ucla, Catherine, Wyss, Carine, Drenkow, Jorg, Dumais, Erica, Murray, Ryan R., Lin, Chenwei, Szeto, David, Denoeud, France, Calvo, Miquel, Frankish, Adam, Harrow, Jennifer, Makrythanasis, Periklis, Vidal, Marc, Salehi-Ashtiani, Kourosh, Antonarakis, Stylianos E., Gingeras, Thomas R., Guigó, Roderic |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2713501/ https://www.ncbi.nlm.nih.gov/pubmed/18500348 http://dx.doi.org/10.1038/nmeth.1216 |
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