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Gene Delivery to the Rat Liver Using Cationic Lipid Emulsion/DNA Complex: Comparison between Intra-arterial, Intraportal and Intravenous Administration

OBJECTIVE: To compare the efficiency of intra-arterial, intraportal, and intravenous administration of cationic lipid emulsion/DNA complex, as used for gene transfer to rat liver. MATERIALS AND METHODS: DNA-carrier complex for the in-vivo experiment was prepared by mixing DNA and a cationic lipid em...

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Autores principales: Choi, Bo Yoon, Chung, Jin Wook, Park, Jae Hyung, Kim, Keon Ha, Kim, Young Il, Koh, Young Hwan, Kwon, Jong Won, Lee, Kyoung Ho, Choi, Hyuk Jae, Kim, Tae Woo, Kim, Young Jin, Chung, Hesson, Kwon, Ik Chan, Jeong, Seo Young
Formato: Texto
Lenguaje:English
Publicado: The Korean Radiological Society 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2713884/
https://www.ncbi.nlm.nih.gov/pubmed/12271165
http://dx.doi.org/10.3348/kjr.2002.3.3.194
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author Choi, Bo Yoon
Chung, Jin Wook
Park, Jae Hyung
Kim, Keon Ha
Kim, Young Il
Koh, Young Hwan
Kwon, Jong Won
Lee, Kyoung Ho
Choi, Hyuk Jae
Kim, Tae Woo
Kim, Young Jin
Chung, Hesson
Kwon, Ik Chan
Jeong, Seo Young
author_facet Choi, Bo Yoon
Chung, Jin Wook
Park, Jae Hyung
Kim, Keon Ha
Kim, Young Il
Koh, Young Hwan
Kwon, Jong Won
Lee, Kyoung Ho
Choi, Hyuk Jae
Kim, Tae Woo
Kim, Young Jin
Chung, Hesson
Kwon, Ik Chan
Jeong, Seo Young
author_sort Choi, Bo Yoon
collection PubMed
description OBJECTIVE: To compare the efficiency of intra-arterial, intraportal, and intravenous administration of cationic lipid emulsion/DNA complex, as used for gene transfer to rat liver. MATERIALS AND METHODS: DNA-carrier complex for the in-vivo experiment was prepared by mixing DNA and a cationic lipid emulsion. According to the administration route used (intra-arterial, intraportal, or intravenous), the animals were assigned to one of three groups. The heart, lung, liver, spleen and kidneys were removed and assayed for total protein and luciferase concentration. RESULTS: The cationic lipid emulsion/DNA complex used successfully transfected the various organs via the different administration routes employed. Luciferase activity in each organ of untreated animals was negligible. Liver luciferase values were significantly higher in the groups in which intra-arterial or intraportal administration was used. CONCLUSION: The intra-arterial or intraportal administration of cationic lipid emulsion/DNA complex is superior to intravenous administration and allows selective gene transfer to the liver.
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spelling pubmed-27138842009-07-23 Gene Delivery to the Rat Liver Using Cationic Lipid Emulsion/DNA Complex: Comparison between Intra-arterial, Intraportal and Intravenous Administration Choi, Bo Yoon Chung, Jin Wook Park, Jae Hyung Kim, Keon Ha Kim, Young Il Koh, Young Hwan Kwon, Jong Won Lee, Kyoung Ho Choi, Hyuk Jae Kim, Tae Woo Kim, Young Jin Chung, Hesson Kwon, Ik Chan Jeong, Seo Young Korean J Radiol Original Article OBJECTIVE: To compare the efficiency of intra-arterial, intraportal, and intravenous administration of cationic lipid emulsion/DNA complex, as used for gene transfer to rat liver. MATERIALS AND METHODS: DNA-carrier complex for the in-vivo experiment was prepared by mixing DNA and a cationic lipid emulsion. According to the administration route used (intra-arterial, intraportal, or intravenous), the animals were assigned to one of three groups. The heart, lung, liver, spleen and kidneys were removed and assayed for total protein and luciferase concentration. RESULTS: The cationic lipid emulsion/DNA complex used successfully transfected the various organs via the different administration routes employed. Luciferase activity in each organ of untreated animals was negligible. Liver luciferase values were significantly higher in the groups in which intra-arterial or intraportal administration was used. CONCLUSION: The intra-arterial or intraportal administration of cationic lipid emulsion/DNA complex is superior to intravenous administration and allows selective gene transfer to the liver. The Korean Radiological Society 2002 2002-09-30 /pmc/articles/PMC2713884/ /pubmed/12271165 http://dx.doi.org/10.3348/kjr.2002.3.3.194 Text en Copyright © 2002 The Korean Radiological Society http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Choi, Bo Yoon
Chung, Jin Wook
Park, Jae Hyung
Kim, Keon Ha
Kim, Young Il
Koh, Young Hwan
Kwon, Jong Won
Lee, Kyoung Ho
Choi, Hyuk Jae
Kim, Tae Woo
Kim, Young Jin
Chung, Hesson
Kwon, Ik Chan
Jeong, Seo Young
Gene Delivery to the Rat Liver Using Cationic Lipid Emulsion/DNA Complex: Comparison between Intra-arterial, Intraportal and Intravenous Administration
title Gene Delivery to the Rat Liver Using Cationic Lipid Emulsion/DNA Complex: Comparison between Intra-arterial, Intraportal and Intravenous Administration
title_full Gene Delivery to the Rat Liver Using Cationic Lipid Emulsion/DNA Complex: Comparison between Intra-arterial, Intraportal and Intravenous Administration
title_fullStr Gene Delivery to the Rat Liver Using Cationic Lipid Emulsion/DNA Complex: Comparison between Intra-arterial, Intraportal and Intravenous Administration
title_full_unstemmed Gene Delivery to the Rat Liver Using Cationic Lipid Emulsion/DNA Complex: Comparison between Intra-arterial, Intraportal and Intravenous Administration
title_short Gene Delivery to the Rat Liver Using Cationic Lipid Emulsion/DNA Complex: Comparison between Intra-arterial, Intraportal and Intravenous Administration
title_sort gene delivery to the rat liver using cationic lipid emulsion/dna complex: comparison between intra-arterial, intraportal and intravenous administration
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2713884/
https://www.ncbi.nlm.nih.gov/pubmed/12271165
http://dx.doi.org/10.3348/kjr.2002.3.3.194
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