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Gene Delivery to the Rat Liver Using Cationic Lipid Emulsion/DNA Complex: Comparison between Intra-arterial, Intraportal and Intravenous Administration
OBJECTIVE: To compare the efficiency of intra-arterial, intraportal, and intravenous administration of cationic lipid emulsion/DNA complex, as used for gene transfer to rat liver. MATERIALS AND METHODS: DNA-carrier complex for the in-vivo experiment was prepared by mixing DNA and a cationic lipid em...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Korean Radiological Society
2002
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2713884/ https://www.ncbi.nlm.nih.gov/pubmed/12271165 http://dx.doi.org/10.3348/kjr.2002.3.3.194 |
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author | Choi, Bo Yoon Chung, Jin Wook Park, Jae Hyung Kim, Keon Ha Kim, Young Il Koh, Young Hwan Kwon, Jong Won Lee, Kyoung Ho Choi, Hyuk Jae Kim, Tae Woo Kim, Young Jin Chung, Hesson Kwon, Ik Chan Jeong, Seo Young |
author_facet | Choi, Bo Yoon Chung, Jin Wook Park, Jae Hyung Kim, Keon Ha Kim, Young Il Koh, Young Hwan Kwon, Jong Won Lee, Kyoung Ho Choi, Hyuk Jae Kim, Tae Woo Kim, Young Jin Chung, Hesson Kwon, Ik Chan Jeong, Seo Young |
author_sort | Choi, Bo Yoon |
collection | PubMed |
description | OBJECTIVE: To compare the efficiency of intra-arterial, intraportal, and intravenous administration of cationic lipid emulsion/DNA complex, as used for gene transfer to rat liver. MATERIALS AND METHODS: DNA-carrier complex for the in-vivo experiment was prepared by mixing DNA and a cationic lipid emulsion. According to the administration route used (intra-arterial, intraportal, or intravenous), the animals were assigned to one of three groups. The heart, lung, liver, spleen and kidneys were removed and assayed for total protein and luciferase concentration. RESULTS: The cationic lipid emulsion/DNA complex used successfully transfected the various organs via the different administration routes employed. Luciferase activity in each organ of untreated animals was negligible. Liver luciferase values were significantly higher in the groups in which intra-arterial or intraportal administration was used. CONCLUSION: The intra-arterial or intraportal administration of cationic lipid emulsion/DNA complex is superior to intravenous administration and allows selective gene transfer to the liver. |
format | Text |
id | pubmed-2713884 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2002 |
publisher | The Korean Radiological Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-27138842009-07-23 Gene Delivery to the Rat Liver Using Cationic Lipid Emulsion/DNA Complex: Comparison between Intra-arterial, Intraportal and Intravenous Administration Choi, Bo Yoon Chung, Jin Wook Park, Jae Hyung Kim, Keon Ha Kim, Young Il Koh, Young Hwan Kwon, Jong Won Lee, Kyoung Ho Choi, Hyuk Jae Kim, Tae Woo Kim, Young Jin Chung, Hesson Kwon, Ik Chan Jeong, Seo Young Korean J Radiol Original Article OBJECTIVE: To compare the efficiency of intra-arterial, intraportal, and intravenous administration of cationic lipid emulsion/DNA complex, as used for gene transfer to rat liver. MATERIALS AND METHODS: DNA-carrier complex for the in-vivo experiment was prepared by mixing DNA and a cationic lipid emulsion. According to the administration route used (intra-arterial, intraportal, or intravenous), the animals were assigned to one of three groups. The heart, lung, liver, spleen and kidneys were removed and assayed for total protein and luciferase concentration. RESULTS: The cationic lipid emulsion/DNA complex used successfully transfected the various organs via the different administration routes employed. Luciferase activity in each organ of untreated animals was negligible. Liver luciferase values were significantly higher in the groups in which intra-arterial or intraportal administration was used. CONCLUSION: The intra-arterial or intraportal administration of cationic lipid emulsion/DNA complex is superior to intravenous administration and allows selective gene transfer to the liver. The Korean Radiological Society 2002 2002-09-30 /pmc/articles/PMC2713884/ /pubmed/12271165 http://dx.doi.org/10.3348/kjr.2002.3.3.194 Text en Copyright © 2002 The Korean Radiological Society http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Choi, Bo Yoon Chung, Jin Wook Park, Jae Hyung Kim, Keon Ha Kim, Young Il Koh, Young Hwan Kwon, Jong Won Lee, Kyoung Ho Choi, Hyuk Jae Kim, Tae Woo Kim, Young Jin Chung, Hesson Kwon, Ik Chan Jeong, Seo Young Gene Delivery to the Rat Liver Using Cationic Lipid Emulsion/DNA Complex: Comparison between Intra-arterial, Intraportal and Intravenous Administration |
title | Gene Delivery to the Rat Liver Using Cationic Lipid Emulsion/DNA Complex: Comparison between Intra-arterial, Intraportal and Intravenous Administration |
title_full | Gene Delivery to the Rat Liver Using Cationic Lipid Emulsion/DNA Complex: Comparison between Intra-arterial, Intraportal and Intravenous Administration |
title_fullStr | Gene Delivery to the Rat Liver Using Cationic Lipid Emulsion/DNA Complex: Comparison between Intra-arterial, Intraportal and Intravenous Administration |
title_full_unstemmed | Gene Delivery to the Rat Liver Using Cationic Lipid Emulsion/DNA Complex: Comparison between Intra-arterial, Intraportal and Intravenous Administration |
title_short | Gene Delivery to the Rat Liver Using Cationic Lipid Emulsion/DNA Complex: Comparison between Intra-arterial, Intraportal and Intravenous Administration |
title_sort | gene delivery to the rat liver using cationic lipid emulsion/dna complex: comparison between intra-arterial, intraportal and intravenous administration |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2713884/ https://www.ncbi.nlm.nih.gov/pubmed/12271165 http://dx.doi.org/10.3348/kjr.2002.3.3.194 |
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