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Construction and analysis of cotton (Gossypium arboreum L.) drought-related cDNA library

BACKGROUND: Drought is one of the most important environmental factors causing water stress for cotton, and it greatly limits cotton growth and crop productivity. So far only a few drought-tolerance genes have been functionally characterized in details, and most efforts on this topic have been made...

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Autores principales: Zhang, Ling, Li, Fu-Guang, Liu, Chuan-Liang, Zhang, Chao-Jun, Zhang, Xue-Yan
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2714314/
https://www.ncbi.nlm.nih.gov/pubmed/19570239
http://dx.doi.org/10.1186/1756-0500-2-120
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author Zhang, Ling
Li, Fu-Guang
Liu, Chuan-Liang
Zhang, Chao-Jun
Zhang, Xue-Yan
author_facet Zhang, Ling
Li, Fu-Guang
Liu, Chuan-Liang
Zhang, Chao-Jun
Zhang, Xue-Yan
author_sort Zhang, Ling
collection PubMed
description BACKGROUND: Drought is one of the most important environmental factors causing water stress for cotton, and it greatly limits cotton growth and crop productivity. So far only a few drought-tolerance genes have been functionally characterized in details, and most efforts on this topic have been made in model organisms. Therefore, to identify more drought-related genes in cotton plays a crucial role in elucidating the underlying mechanisms of drought tolerance as well as utilizing bioengineering techniques to improve the tolerance in this organism. FINDINGS: Here we constructed a subtractive drought-tolerance cDNA library using suppressive subtractive hybridization (SSH). Through differential screening and bioinformatics analysis, we identified 392 positive clones with differential expression, corresponding 265 unique genes. By BLAST search against Genbank, we found that more than half of these EST sequences were homologous to those previously known drought-related genes and that there were 57 sequences with unknown functions, suggesting that many more genes are involved in this complex trait. Moreover, using RT-PCR, we examined the expression of nine representative candidate genes and confirmed that their expression levels were increased at different levels under drought stress. CONCLUSION: Our results show that drought tolerance is a complex trait in cotton, which involves the coordination of many genes and multiple metabolism pathways. The candidate EST sequences we identified here would facilitate further functional studies of drought-related genes and provide important insights into the molecular mechanisms of drought-stress tolerance and genetic breeding in cotton.
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spelling pubmed-27143142009-07-23 Construction and analysis of cotton (Gossypium arboreum L.) drought-related cDNA library Zhang, Ling Li, Fu-Guang Liu, Chuan-Liang Zhang, Chao-Jun Zhang, Xue-Yan BMC Res Notes Technical Note BACKGROUND: Drought is one of the most important environmental factors causing water stress for cotton, and it greatly limits cotton growth and crop productivity. So far only a few drought-tolerance genes have been functionally characterized in details, and most efforts on this topic have been made in model organisms. Therefore, to identify more drought-related genes in cotton plays a crucial role in elucidating the underlying mechanisms of drought tolerance as well as utilizing bioengineering techniques to improve the tolerance in this organism. FINDINGS: Here we constructed a subtractive drought-tolerance cDNA library using suppressive subtractive hybridization (SSH). Through differential screening and bioinformatics analysis, we identified 392 positive clones with differential expression, corresponding 265 unique genes. By BLAST search against Genbank, we found that more than half of these EST sequences were homologous to those previously known drought-related genes and that there were 57 sequences with unknown functions, suggesting that many more genes are involved in this complex trait. Moreover, using RT-PCR, we examined the expression of nine representative candidate genes and confirmed that their expression levels were increased at different levels under drought stress. CONCLUSION: Our results show that drought tolerance is a complex trait in cotton, which involves the coordination of many genes and multiple metabolism pathways. The candidate EST sequences we identified here would facilitate further functional studies of drought-related genes and provide important insights into the molecular mechanisms of drought-stress tolerance and genetic breeding in cotton. BioMed Central 2009-07-02 /pmc/articles/PMC2714314/ /pubmed/19570239 http://dx.doi.org/10.1186/1756-0500-2-120 Text en Copyright © 2009 Li et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Technical Note
Zhang, Ling
Li, Fu-Guang
Liu, Chuan-Liang
Zhang, Chao-Jun
Zhang, Xue-Yan
Construction and analysis of cotton (Gossypium arboreum L.) drought-related cDNA library
title Construction and analysis of cotton (Gossypium arboreum L.) drought-related cDNA library
title_full Construction and analysis of cotton (Gossypium arboreum L.) drought-related cDNA library
title_fullStr Construction and analysis of cotton (Gossypium arboreum L.) drought-related cDNA library
title_full_unstemmed Construction and analysis of cotton (Gossypium arboreum L.) drought-related cDNA library
title_short Construction and analysis of cotton (Gossypium arboreum L.) drought-related cDNA library
title_sort construction and analysis of cotton (gossypium arboreum l.) drought-related cdna library
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2714314/
https://www.ncbi.nlm.nih.gov/pubmed/19570239
http://dx.doi.org/10.1186/1756-0500-2-120
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