Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA

We describe an improvement of an earlier reported real-time RT-PCR assay for the detection of enterovirus RNA, based on the 5' exonuclease digestion of a dual-labeled fluorogenic probe by Taq DNA polymerase. A different extraction method, real-time RT-PCR instrument and primer set were evaluate...

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Detalles Bibliográficos
Autores principales: Piqueur, Marian AC, Verstrepen, Walter A, Bruynseels, Peggy, Mertens, An H
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2716329/
https://www.ncbi.nlm.nih.gov/pubmed/19583870
http://dx.doi.org/10.1186/1743-422X-6-95
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author Piqueur, Marian AC
Verstrepen, Walter A
Bruynseels, Peggy
Mertens, An H
author_facet Piqueur, Marian AC
Verstrepen, Walter A
Bruynseels, Peggy
Mertens, An H
author_sort Piqueur, Marian AC
collection PubMed
description We describe an improvement of an earlier reported real-time RT-PCR assay for the detection of enterovirus RNA, based on the 5' exonuclease digestion of a dual-labeled fluorogenic probe by Taq DNA polymerase. A different extraction method, real-time RT-PCR instrument and primer set were evaluated. Our data show that the optimized assay yields a higher sensitivity and reproducibility and resulted in a significant reduced hands-on time per sample.
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spelling pubmed-27163292009-07-28 Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA Piqueur, Marian AC Verstrepen, Walter A Bruynseels, Peggy Mertens, An H Virol J Short Report We describe an improvement of an earlier reported real-time RT-PCR assay for the detection of enterovirus RNA, based on the 5' exonuclease digestion of a dual-labeled fluorogenic probe by Taq DNA polymerase. A different extraction method, real-time RT-PCR instrument and primer set were evaluated. Our data show that the optimized assay yields a higher sensitivity and reproducibility and resulted in a significant reduced hands-on time per sample. BioMed Central 2009-07-07 /pmc/articles/PMC2716329/ /pubmed/19583870 http://dx.doi.org/10.1186/1743-422X-6-95 Text en Copyright © 2009 Piqueur et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Piqueur, Marian AC
Verstrepen, Walter A
Bruynseels, Peggy
Mertens, An H
Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA
title Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA
title_full Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA
title_fullStr Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA
title_full_unstemmed Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA
title_short Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA
title_sort improvement of a real-time rt-pcr assay for the detection of enterovirus rna
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2716329/
https://www.ncbi.nlm.nih.gov/pubmed/19583870
http://dx.doi.org/10.1186/1743-422X-6-95
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AT mertensanh improvementofarealtimertpcrassayforthedetectionofenterovirusrna

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