Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA

We describe an improvement of an earlier reported real-time RT-PCR assay for the detection of enterovirus RNA, based on the 5' exonuclease digestion of a dual-labeled fluorogenic probe by Taq DNA polymerase. A different extraction method, real-time RT-PCR instrument and primer set were evaluate...

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Detalles Bibliográficos
Autores principales: Piqueur, Marian AC, Verstrepen, Walter A, Bruynseels, Peggy, Mertens, An H
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2716329/
https://www.ncbi.nlm.nih.gov/pubmed/19583870
http://dx.doi.org/10.1186/1743-422X-6-95

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