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Possible Impact of Salivary Influence on Cytokine Analysis in Exhaled Breath Condensate
BACKGROUND: Exhaled breath condensate (EBC) is thought to contain substances of the lower airway epithelial lining fluid (ELF) aerosolized by turbulent flow. However, contamination by saliva may affect the EBC when collected orally. OBJECTIVE: The purpose of this study was to compare the cytokine ex...
Autores principales: | , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Libertas Academica
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2716811/ https://www.ncbi.nlm.nih.gov/pubmed/19662182 |
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author | Ichikawa, T. Matsunaga, K. Minakata, Y. Yanagisawa, S. Ueshima, K. Akamatsu, K. Hirano, T. Nakanishi, M. Sugiura, H. Yamagata, T. Ichinose, M. |
author_facet | Ichikawa, T. Matsunaga, K. Minakata, Y. Yanagisawa, S. Ueshima, K. Akamatsu, K. Hirano, T. Nakanishi, M. Sugiura, H. Yamagata, T. Ichinose, M. |
author_sort | Ichikawa, T. |
collection | PubMed |
description | BACKGROUND: Exhaled breath condensate (EBC) is thought to contain substances of the lower airway epithelial lining fluid (ELF) aerosolized by turbulent flow. However, contamination by saliva may affect the EBC when collected orally. OBJECTIVE: The purpose of this study was to compare the cytokine expression levels in EBC with those in saliva, and to clarify the influence of saliva on cytokine measurements of EBC. METHODS: EBC and saliva samples were obtained from 10 adult subjects with stable asthma. To estimate differences in the contents of substances between EBC and saliva, the total protein concentration of each sample was measured. Further, we also measured the total protein concentration of ELF obtained from another patient group with suspected lung cancer using a micro sampling probe during bronchoscopic examination and roughly estimated the dilution of EBC by comparing the total protein concentration of EBC and ELF from those two patient groups. The cytokine expression levels of EBC and saliva from asthmatic group were assessed by a cytokine protein array. RESULTS: The mean total protein concentrations in EBC, saliva and ELF were 4.6 μg/ml, 2,398 μg/ml and 14,111 μg/ml, respectively. The dilution of EBC could be estimated as 1:3000. Forty cytokines were analyzed by a cytokine protein array and each cytokine expression level of EBC was found to be different from that of saliva. Corrected by the total protein concentration, all cytokine expression levels of EBC were significantly higher than those of saliva. CONCLUSION: These results suggest that the salivary influence on the cytokine assessment in EBC may be negligible. |
format | Text |
id | pubmed-2716811 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Libertas Academica |
record_format | MEDLINE/PubMed |
spelling | pubmed-27168112009-08-06 Possible Impact of Salivary Influence on Cytokine Analysis in Exhaled Breath Condensate Ichikawa, T. Matsunaga, K. Minakata, Y. Yanagisawa, S. Ueshima, K. Akamatsu, K. Hirano, T. Nakanishi, M. Sugiura, H. Yamagata, T. Ichinose, M. Anal Chem Insights Original Research BACKGROUND: Exhaled breath condensate (EBC) is thought to contain substances of the lower airway epithelial lining fluid (ELF) aerosolized by turbulent flow. However, contamination by saliva may affect the EBC when collected orally. OBJECTIVE: The purpose of this study was to compare the cytokine expression levels in EBC with those in saliva, and to clarify the influence of saliva on cytokine measurements of EBC. METHODS: EBC and saliva samples were obtained from 10 adult subjects with stable asthma. To estimate differences in the contents of substances between EBC and saliva, the total protein concentration of each sample was measured. Further, we also measured the total protein concentration of ELF obtained from another patient group with suspected lung cancer using a micro sampling probe during bronchoscopic examination and roughly estimated the dilution of EBC by comparing the total protein concentration of EBC and ELF from those two patient groups. The cytokine expression levels of EBC and saliva from asthmatic group were assessed by a cytokine protein array. RESULTS: The mean total protein concentrations in EBC, saliva and ELF were 4.6 μg/ml, 2,398 μg/ml and 14,111 μg/ml, respectively. The dilution of EBC could be estimated as 1:3000. Forty cytokines were analyzed by a cytokine protein array and each cytokine expression level of EBC was found to be different from that of saliva. Corrected by the total protein concentration, all cytokine expression levels of EBC were significantly higher than those of saliva. CONCLUSION: These results suggest that the salivary influence on the cytokine assessment in EBC may be negligible. Libertas Academica 2007-10-12 /pmc/articles/PMC2716811/ /pubmed/19662182 Text en Copyright © 2007 The authors. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Original Research Ichikawa, T. Matsunaga, K. Minakata, Y. Yanagisawa, S. Ueshima, K. Akamatsu, K. Hirano, T. Nakanishi, M. Sugiura, H. Yamagata, T. Ichinose, M. Possible Impact of Salivary Influence on Cytokine Analysis in Exhaled Breath Condensate |
title | Possible Impact of Salivary Influence on Cytokine Analysis in Exhaled Breath Condensate |
title_full | Possible Impact of Salivary Influence on Cytokine Analysis in Exhaled Breath Condensate |
title_fullStr | Possible Impact of Salivary Influence on Cytokine Analysis in Exhaled Breath Condensate |
title_full_unstemmed | Possible Impact of Salivary Influence on Cytokine Analysis in Exhaled Breath Condensate |
title_short | Possible Impact of Salivary Influence on Cytokine Analysis in Exhaled Breath Condensate |
title_sort | possible impact of salivary influence on cytokine analysis in exhaled breath condensate |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2716811/ https://www.ncbi.nlm.nih.gov/pubmed/19662182 |
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