Identification of Cysteine Proteases and Screening of Cysteine Protease Inhibitors in Biological Samples by a Two-Dimensional Gel System of Zymography and Reverse Zymography

We have developed a two-dimensional (2D-) gel system of zymography and reverse zymography for the detection and characterization of proteases and protease inhibitors. Isoelectric focusing (IEF) agarose gels with pH gradients were employed for separation in the first-dimension and sodium dodecyl sulf...

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Detalles Bibliográficos
Autores principales: Saitoh, Eiichi, Yamamoto, Shinya, Okamoto, Eishiro, Hayakawa, Yoshimi, Hoshino, Takashi, Sato, Ritsuko, Isemura, Satoko, Ohtsubo, Sadami, Taniguchi, Masayuki
Formato: Texto
Lenguaje:English
Publicado: Libertas Academica 2007
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2716818/
https://www.ncbi.nlm.nih.gov/pubmed/19662177

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