Size-exclusion chromatography can identify faster-associating protein complexes and evaluate design strategies

BACKGROUND: We previously developed a set of rationally designed mutant MICA protein ligands for the NKG2D immunoreceptor in which MICA was mutated at residues that do not contact NKG2D. Some of these MICA mutants, predicted by RosettaDesign to be destabilized, bound NKG2D with affinities enhanced b...

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Detalles Bibliográficos
Autores principales: Mayer, Chad L, Snyder, W Kalani, Swietlicka, Monika A, VanSchoiack, Andrew D, Austin, Chad R, McFarland, Benjamin J
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2717102/
https://www.ncbi.nlm.nih.gov/pubmed/19604395
http://dx.doi.org/10.1186/1756-0500-2-135

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