SUMOylation of nuclear actin

Actin, a major component of the cytoplasm, is also abundant in the nucleus. Nuclear actin is involved in a variety of nuclear processes including transcription, chromatin remodeling, and intranuclear transport. Nevertheless, the regulation of nuclear actin by posttranslational modifications has not...

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Autores principales: Hofmann, Wilma A., Arduini, Alessandro, Nicol, Samantha M., Camacho, Carlos J., Lessard, James L., Fuller-Pace, Frances V., de Lanerolle, Primal
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2009
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2717643/
https://www.ncbi.nlm.nih.gov/pubmed/19635839
http://dx.doi.org/10.1083/jcb.200905016
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author Hofmann, Wilma A.
Arduini, Alessandro
Nicol, Samantha M.
Camacho, Carlos J.
Lessard, James L.
Fuller-Pace, Frances V.
de Lanerolle, Primal
author_facet Hofmann, Wilma A.
Arduini, Alessandro
Nicol, Samantha M.
Camacho, Carlos J.
Lessard, James L.
Fuller-Pace, Frances V.
de Lanerolle, Primal
author_sort Hofmann, Wilma A.
collection PubMed
description Actin, a major component of the cytoplasm, is also abundant in the nucleus. Nuclear actin is involved in a variety of nuclear processes including transcription, chromatin remodeling, and intranuclear transport. Nevertheless, the regulation of nuclear actin by posttranslational modifications has not been investigated. We now show that nuclear actin is modified by SUMO2 and SUMO3 and that computational modeling and site-directed mutagenesis identified K68 and K284 as critical sites for SUMOylating actin. We also present a model for the actin–SUMO complex and show that SUMOylation is required for the nuclear localization of actin.
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spelling pubmed-27176432010-01-27 SUMOylation of nuclear actin Hofmann, Wilma A. Arduini, Alessandro Nicol, Samantha M. Camacho, Carlos J. Lessard, James L. Fuller-Pace, Frances V. de Lanerolle, Primal J Cell Biol Research Articles Actin, a major component of the cytoplasm, is also abundant in the nucleus. Nuclear actin is involved in a variety of nuclear processes including transcription, chromatin remodeling, and intranuclear transport. Nevertheless, the regulation of nuclear actin by posttranslational modifications has not been investigated. We now show that nuclear actin is modified by SUMO2 and SUMO3 and that computational modeling and site-directed mutagenesis identified K68 and K284 as critical sites for SUMOylating actin. We also present a model for the actin–SUMO complex and show that SUMOylation is required for the nuclear localization of actin. The Rockefeller University Press 2009-07-27 /pmc/articles/PMC2717643/ /pubmed/19635839 http://dx.doi.org/10.1083/jcb.200905016 Text en © 2009 Hofmann et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Hofmann, Wilma A.
Arduini, Alessandro
Nicol, Samantha M.
Camacho, Carlos J.
Lessard, James L.
Fuller-Pace, Frances V.
de Lanerolle, Primal
SUMOylation of nuclear actin
title SUMOylation of nuclear actin
title_full SUMOylation of nuclear actin
title_fullStr SUMOylation of nuclear actin
title_full_unstemmed SUMOylation of nuclear actin
title_short SUMOylation of nuclear actin
title_sort sumoylation of nuclear actin
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2717643/
https://www.ncbi.nlm.nih.gov/pubmed/19635839
http://dx.doi.org/10.1083/jcb.200905016
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AT camachocarlosj sumoylationofnuclearactin
AT lessardjamesl sumoylationofnuclearactin
AT fullerpacefrancesv sumoylationofnuclearactin
AT delanerolleprimal sumoylationofnuclearactin

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