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Cohesin regulates VSG monoallelic expression in trypanosomes
Antigenic variation allows Trypanosoma brucei to evade the host immune response by switching the expression of 1 out of ∼15 telomeric variant surface glycoprotein (VSG) expression sites (ESs). VSG ES transcription is mediated by RNA polymerase I in a discrete nuclear site named the ES body (ESB). Ho...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2717648/ https://www.ncbi.nlm.nih.gov/pubmed/19635842 http://dx.doi.org/10.1083/jcb.200902119 |
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author | Landeira, David Bart, Jean-Mathieu Van Tyne, Daria Navarro, Miguel |
author_facet | Landeira, David Bart, Jean-Mathieu Van Tyne, Daria Navarro, Miguel |
author_sort | Landeira, David |
collection | PubMed |
description | Antigenic variation allows Trypanosoma brucei to evade the host immune response by switching the expression of 1 out of ∼15 telomeric variant surface glycoprotein (VSG) expression sites (ESs). VSG ES transcription is mediated by RNA polymerase I in a discrete nuclear site named the ES body (ESB). However, nothing is known about how the monoallelic VSG ES transcriptional state is maintained over generations. In this study, we show that during S and G2 phases and early mitosis, the active VSG ES locus remains associated with the single ESB and exhibits a delay in the separation of sister chromatids relative to control loci. This delay is dependent on the cohesin complex, as partial knockdown of cohesin subunits resulted in premature separation of sister chromatids of the active VSG ES. Cohesin depletion also prompted transcriptional switching from the active to previously inactive VSG ESs. Thus, in addition to maintaining sister chromatid cohesion during mitosis, the cohesin complex plays an essential role in the correct epigenetic inheritance of the active transcriptional VSG ES state. |
format | Text |
id | pubmed-2717648 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-27176482010-01-27 Cohesin regulates VSG monoallelic expression in trypanosomes Landeira, David Bart, Jean-Mathieu Van Tyne, Daria Navarro, Miguel J Cell Biol Research Articles Antigenic variation allows Trypanosoma brucei to evade the host immune response by switching the expression of 1 out of ∼15 telomeric variant surface glycoprotein (VSG) expression sites (ESs). VSG ES transcription is mediated by RNA polymerase I in a discrete nuclear site named the ES body (ESB). However, nothing is known about how the monoallelic VSG ES transcriptional state is maintained over generations. In this study, we show that during S and G2 phases and early mitosis, the active VSG ES locus remains associated with the single ESB and exhibits a delay in the separation of sister chromatids relative to control loci. This delay is dependent on the cohesin complex, as partial knockdown of cohesin subunits resulted in premature separation of sister chromatids of the active VSG ES. Cohesin depletion also prompted transcriptional switching from the active to previously inactive VSG ESs. Thus, in addition to maintaining sister chromatid cohesion during mitosis, the cohesin complex plays an essential role in the correct epigenetic inheritance of the active transcriptional VSG ES state. The Rockefeller University Press 2009-07-27 /pmc/articles/PMC2717648/ /pubmed/19635842 http://dx.doi.org/10.1083/jcb.200902119 Text en © 2009 Landeira et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Landeira, David Bart, Jean-Mathieu Van Tyne, Daria Navarro, Miguel Cohesin regulates VSG monoallelic expression in trypanosomes |
title | Cohesin regulates VSG monoallelic expression in trypanosomes |
title_full | Cohesin regulates VSG monoallelic expression in trypanosomes |
title_fullStr | Cohesin regulates VSG monoallelic expression in trypanosomes |
title_full_unstemmed | Cohesin regulates VSG monoallelic expression in trypanosomes |
title_short | Cohesin regulates VSG monoallelic expression in trypanosomes |
title_sort | cohesin regulates vsg monoallelic expression in trypanosomes |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2717648/ https://www.ncbi.nlm.nih.gov/pubmed/19635842 http://dx.doi.org/10.1083/jcb.200902119 |
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