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The CENP-S complex is essential for the stable assembly of outer kinetochore structure

The constitutive centromere-associated network (CCAN) proteins are central to kinetochore assembly. To define the molecular architecture of this critical kinetochore network, we sought to determine the full complement of CCAN components and to define their relationships. This work identified a centr...

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Autores principales: Amano, Miho, Suzuki, Aussie, Hori, Tetsuya, Backer, Chelsea, Okawa, Katsuya, Cheeseman, Iain M., Fukagawa, Tatsuo
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2717651/
https://www.ncbi.nlm.nih.gov/pubmed/19620631
http://dx.doi.org/10.1083/jcb.200903100
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author Amano, Miho
Suzuki, Aussie
Hori, Tetsuya
Backer, Chelsea
Okawa, Katsuya
Cheeseman, Iain M.
Fukagawa, Tatsuo
author_facet Amano, Miho
Suzuki, Aussie
Hori, Tetsuya
Backer, Chelsea
Okawa, Katsuya
Cheeseman, Iain M.
Fukagawa, Tatsuo
author_sort Amano, Miho
collection PubMed
description The constitutive centromere-associated network (CCAN) proteins are central to kinetochore assembly. To define the molecular architecture of this critical kinetochore network, we sought to determine the full complement of CCAN components and to define their relationships. This work identified a centromere protein S (CENP-S)–containing subcomplex that includes the new constitutive kinetochore protein CENP-X. Both CENP-S– and CENP-X–deficient chicken DT40 cells are viable but show abnormal mitotic behavior based on live cell analysis. Human HeLa cells depleted for CENP-X also showed mitotic errors. The kinetochore localization of CENP-S and -X is abolished in CENP-T– or CENP-K–deficient cells, but reciprocal experiments using CENP-S–deficient cells did not reveal defects in the localization of CCAN components. However, CENP-S– and CENP-X–deficient cells show a significant reduction in the size of the kinetochore outer plate. In addition, we found that intrakinetochore distance was increased in CENP-S– and CENP-X–deficient cells. These results suggest that the CENP-S complex is essential for the stable assembly of the outer kinetochore.
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spelling pubmed-27176512010-01-27 The CENP-S complex is essential for the stable assembly of outer kinetochore structure Amano, Miho Suzuki, Aussie Hori, Tetsuya Backer, Chelsea Okawa, Katsuya Cheeseman, Iain M. Fukagawa, Tatsuo J Cell Biol Research Articles The constitutive centromere-associated network (CCAN) proteins are central to kinetochore assembly. To define the molecular architecture of this critical kinetochore network, we sought to determine the full complement of CCAN components and to define their relationships. This work identified a centromere protein S (CENP-S)–containing subcomplex that includes the new constitutive kinetochore protein CENP-X. Both CENP-S– and CENP-X–deficient chicken DT40 cells are viable but show abnormal mitotic behavior based on live cell analysis. Human HeLa cells depleted for CENP-X also showed mitotic errors. The kinetochore localization of CENP-S and -X is abolished in CENP-T– or CENP-K–deficient cells, but reciprocal experiments using CENP-S–deficient cells did not reveal defects in the localization of CCAN components. However, CENP-S– and CENP-X–deficient cells show a significant reduction in the size of the kinetochore outer plate. In addition, we found that intrakinetochore distance was increased in CENP-S– and CENP-X–deficient cells. These results suggest that the CENP-S complex is essential for the stable assembly of the outer kinetochore. The Rockefeller University Press 2009-07-27 /pmc/articles/PMC2717651/ /pubmed/19620631 http://dx.doi.org/10.1083/jcb.200903100 Text en © 2009 Amano et al. https://creativecommons.org/licenses/by-nc-sa/3.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ (https://creativecommons.org/licenses/by-nc-sa/3.0/) ).
spellingShingle Research Articles
Amano, Miho
Suzuki, Aussie
Hori, Tetsuya
Backer, Chelsea
Okawa, Katsuya
Cheeseman, Iain M.
Fukagawa, Tatsuo
The CENP-S complex is essential for the stable assembly of outer kinetochore structure
title The CENP-S complex is essential for the stable assembly of outer kinetochore structure
title_full The CENP-S complex is essential for the stable assembly of outer kinetochore structure
title_fullStr The CENP-S complex is essential for the stable assembly of outer kinetochore structure
title_full_unstemmed The CENP-S complex is essential for the stable assembly of outer kinetochore structure
title_short The CENP-S complex is essential for the stable assembly of outer kinetochore structure
title_sort cenp-s complex is essential for the stable assembly of outer kinetochore structure
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2717651/
https://www.ncbi.nlm.nih.gov/pubmed/19620631
http://dx.doi.org/10.1083/jcb.200903100
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