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Characterization of regulatory T cells in urban newborns

BACKGROUND: In the United States, asthma prevalence is particularly high among urban children. Although the underlying immune mechanism contributing to asthma has not been identified, having impaired T regulatory (Treg) cells at birth may be a determining factor in urban children. The objective of t...

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Autores principales: Ly, Ngoc P, Ruiz-Perez, Begona, McLoughlin, Rachel M, Visness, Cynthia M, Wallace, Paul K, Cruikshank, William W, Tzianabos, Arthur O, O'Connor, George T, Gold, Diane R, Gern, James E
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2717905/
https://www.ncbi.nlm.nih.gov/pubmed/19586545
http://dx.doi.org/10.1186/1476-7961-7-8
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author Ly, Ngoc P
Ruiz-Perez, Begona
McLoughlin, Rachel M
Visness, Cynthia M
Wallace, Paul K
Cruikshank, William W
Tzianabos, Arthur O
O'Connor, George T
Gold, Diane R
Gern, James E
author_facet Ly, Ngoc P
Ruiz-Perez, Begona
McLoughlin, Rachel M
Visness, Cynthia M
Wallace, Paul K
Cruikshank, William W
Tzianabos, Arthur O
O'Connor, George T
Gold, Diane R
Gern, James E
author_sort Ly, Ngoc P
collection PubMed
description BACKGROUND: In the United States, asthma prevalence is particularly high among urban children. Although the underlying immune mechanism contributing to asthma has not been identified, having impaired T regulatory (Treg) cells at birth may be a determining factor in urban children. The objective of this study was to compare Treg phenotype and function in cord blood (CB) of newborns to those in peripheral blood (PB) of a subset of participating mothers. METHODS: Treg numbers, expression, and suppressive function were quantified in subjects recruited prenatally from neighborhoods where ≥ 20% of families have incomes below the poverty line. Proportion of Treg cells and expression of naïve (CD45RA) or activated (CD45RO, CD69, and HLA-DR) markers in CD4(+)T cells was measured by flow cytometry. Treg suppressive capacity was determined by quantifying PHA-stimulated lymphocyte proliferation in mononuclear cell samples with and without CD25 depletion. RESULTS: In an urban cohort of 119 newborns and 82 mothers, we found that newborns had similar number of cells expressing FOXP3 as compared to the mothers but had reduced numbers of CD4(+)CD25(+)bright cells that predominantly expressed the naïve (CD45RA) rather than the activated/memory (CD45RO) phenotype found in the mothers. Additionally, the newborns had reduced mononuclear cell TGF-β production, and reduced Treg suppression of PHA-stimulated lymphocyte proliferation compared to the mothers. CONCLUSION: U.S. urban newborns have Treg cells that express FOXP3, albeit with an immature phenotype and function as compared to the mothers. Longitudinal follow-up is needed to delineate Treg cell maturation and subsequent risk for atopic diseases in this urban birth cohort.
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spelling pubmed-27179052009-07-30 Characterization of regulatory T cells in urban newborns Ly, Ngoc P Ruiz-Perez, Begona McLoughlin, Rachel M Visness, Cynthia M Wallace, Paul K Cruikshank, William W Tzianabos, Arthur O O'Connor, George T Gold, Diane R Gern, James E Clin Mol Allergy Research BACKGROUND: In the United States, asthma prevalence is particularly high among urban children. Although the underlying immune mechanism contributing to asthma has not been identified, having impaired T regulatory (Treg) cells at birth may be a determining factor in urban children. The objective of this study was to compare Treg phenotype and function in cord blood (CB) of newborns to those in peripheral blood (PB) of a subset of participating mothers. METHODS: Treg numbers, expression, and suppressive function were quantified in subjects recruited prenatally from neighborhoods where ≥ 20% of families have incomes below the poverty line. Proportion of Treg cells and expression of naïve (CD45RA) or activated (CD45RO, CD69, and HLA-DR) markers in CD4(+)T cells was measured by flow cytometry. Treg suppressive capacity was determined by quantifying PHA-stimulated lymphocyte proliferation in mononuclear cell samples with and without CD25 depletion. RESULTS: In an urban cohort of 119 newborns and 82 mothers, we found that newborns had similar number of cells expressing FOXP3 as compared to the mothers but had reduced numbers of CD4(+)CD25(+)bright cells that predominantly expressed the naïve (CD45RA) rather than the activated/memory (CD45RO) phenotype found in the mothers. Additionally, the newborns had reduced mononuclear cell TGF-β production, and reduced Treg suppression of PHA-stimulated lymphocyte proliferation compared to the mothers. CONCLUSION: U.S. urban newborns have Treg cells that express FOXP3, albeit with an immature phenotype and function as compared to the mothers. Longitudinal follow-up is needed to delineate Treg cell maturation and subsequent risk for atopic diseases in this urban birth cohort. BioMed Central 2009-07-08 /pmc/articles/PMC2717905/ /pubmed/19586545 http://dx.doi.org/10.1186/1476-7961-7-8 Text en Copyright © 2009 Ly et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Ly, Ngoc P
Ruiz-Perez, Begona
McLoughlin, Rachel M
Visness, Cynthia M
Wallace, Paul K
Cruikshank, William W
Tzianabos, Arthur O
O'Connor, George T
Gold, Diane R
Gern, James E
Characterization of regulatory T cells in urban newborns
title Characterization of regulatory T cells in urban newborns
title_full Characterization of regulatory T cells in urban newborns
title_fullStr Characterization of regulatory T cells in urban newborns
title_full_unstemmed Characterization of regulatory T cells in urban newborns
title_short Characterization of regulatory T cells in urban newborns
title_sort characterization of regulatory t cells in urban newborns
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2717905/
https://www.ncbi.nlm.nih.gov/pubmed/19586545
http://dx.doi.org/10.1186/1476-7961-7-8
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