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Characterization of regulatory T cells in urban newborns
BACKGROUND: In the United States, asthma prevalence is particularly high among urban children. Although the underlying immune mechanism contributing to asthma has not been identified, having impaired T regulatory (Treg) cells at birth may be a determining factor in urban children. The objective of t...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2717905/ https://www.ncbi.nlm.nih.gov/pubmed/19586545 http://dx.doi.org/10.1186/1476-7961-7-8 |
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author | Ly, Ngoc P Ruiz-Perez, Begona McLoughlin, Rachel M Visness, Cynthia M Wallace, Paul K Cruikshank, William W Tzianabos, Arthur O O'Connor, George T Gold, Diane R Gern, James E |
author_facet | Ly, Ngoc P Ruiz-Perez, Begona McLoughlin, Rachel M Visness, Cynthia M Wallace, Paul K Cruikshank, William W Tzianabos, Arthur O O'Connor, George T Gold, Diane R Gern, James E |
author_sort | Ly, Ngoc P |
collection | PubMed |
description | BACKGROUND: In the United States, asthma prevalence is particularly high among urban children. Although the underlying immune mechanism contributing to asthma has not been identified, having impaired T regulatory (Treg) cells at birth may be a determining factor in urban children. The objective of this study was to compare Treg phenotype and function in cord blood (CB) of newborns to those in peripheral blood (PB) of a subset of participating mothers. METHODS: Treg numbers, expression, and suppressive function were quantified in subjects recruited prenatally from neighborhoods where ≥ 20% of families have incomes below the poverty line. Proportion of Treg cells and expression of naïve (CD45RA) or activated (CD45RO, CD69, and HLA-DR) markers in CD4(+)T cells was measured by flow cytometry. Treg suppressive capacity was determined by quantifying PHA-stimulated lymphocyte proliferation in mononuclear cell samples with and without CD25 depletion. RESULTS: In an urban cohort of 119 newborns and 82 mothers, we found that newborns had similar number of cells expressing FOXP3 as compared to the mothers but had reduced numbers of CD4(+)CD25(+)bright cells that predominantly expressed the naïve (CD45RA) rather than the activated/memory (CD45RO) phenotype found in the mothers. Additionally, the newborns had reduced mononuclear cell TGF-β production, and reduced Treg suppression of PHA-stimulated lymphocyte proliferation compared to the mothers. CONCLUSION: U.S. urban newborns have Treg cells that express FOXP3, albeit with an immature phenotype and function as compared to the mothers. Longitudinal follow-up is needed to delineate Treg cell maturation and subsequent risk for atopic diseases in this urban birth cohort. |
format | Text |
id | pubmed-2717905 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-27179052009-07-30 Characterization of regulatory T cells in urban newborns Ly, Ngoc P Ruiz-Perez, Begona McLoughlin, Rachel M Visness, Cynthia M Wallace, Paul K Cruikshank, William W Tzianabos, Arthur O O'Connor, George T Gold, Diane R Gern, James E Clin Mol Allergy Research BACKGROUND: In the United States, asthma prevalence is particularly high among urban children. Although the underlying immune mechanism contributing to asthma has not been identified, having impaired T regulatory (Treg) cells at birth may be a determining factor in urban children. The objective of this study was to compare Treg phenotype and function in cord blood (CB) of newborns to those in peripheral blood (PB) of a subset of participating mothers. METHODS: Treg numbers, expression, and suppressive function were quantified in subjects recruited prenatally from neighborhoods where ≥ 20% of families have incomes below the poverty line. Proportion of Treg cells and expression of naïve (CD45RA) or activated (CD45RO, CD69, and HLA-DR) markers in CD4(+)T cells was measured by flow cytometry. Treg suppressive capacity was determined by quantifying PHA-stimulated lymphocyte proliferation in mononuclear cell samples with and without CD25 depletion. RESULTS: In an urban cohort of 119 newborns and 82 mothers, we found that newborns had similar number of cells expressing FOXP3 as compared to the mothers but had reduced numbers of CD4(+)CD25(+)bright cells that predominantly expressed the naïve (CD45RA) rather than the activated/memory (CD45RO) phenotype found in the mothers. Additionally, the newborns had reduced mononuclear cell TGF-β production, and reduced Treg suppression of PHA-stimulated lymphocyte proliferation compared to the mothers. CONCLUSION: U.S. urban newborns have Treg cells that express FOXP3, albeit with an immature phenotype and function as compared to the mothers. Longitudinal follow-up is needed to delineate Treg cell maturation and subsequent risk for atopic diseases in this urban birth cohort. BioMed Central 2009-07-08 /pmc/articles/PMC2717905/ /pubmed/19586545 http://dx.doi.org/10.1186/1476-7961-7-8 Text en Copyright © 2009 Ly et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Ly, Ngoc P Ruiz-Perez, Begona McLoughlin, Rachel M Visness, Cynthia M Wallace, Paul K Cruikshank, William W Tzianabos, Arthur O O'Connor, George T Gold, Diane R Gern, James E Characterization of regulatory T cells in urban newborns |
title | Characterization of regulatory T cells in urban newborns |
title_full | Characterization of regulatory T cells in urban newborns |
title_fullStr | Characterization of regulatory T cells in urban newborns |
title_full_unstemmed | Characterization of regulatory T cells in urban newborns |
title_short | Characterization of regulatory T cells in urban newborns |
title_sort | characterization of regulatory t cells in urban newborns |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2717905/ https://www.ncbi.nlm.nih.gov/pubmed/19586545 http://dx.doi.org/10.1186/1476-7961-7-8 |
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