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Diversity and activity of sugar transporters in nematode-induced root syncytia

The plant-parasitic nematode Heterodera schachtii stimulates plant root cells to form syncytial feeding structures which synthesize all nutrients required for successful nematode development. Cellular re-arrangements and modified metabolism of the syncytia are accompanied by massive intra- and inter...

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Autores principales: Hofmann, Julia, Hess, Paul H., Szakasits, Dagmar, Blöchl, Andreas, Wieczorek, Krzysztof, Daxböck-Horvath, Sabine, Bohlmann, Holger, van Bel, Aart J. E., Grundler, Florian M. W.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2718214/
https://www.ncbi.nlm.nih.gov/pubmed/19487386
http://dx.doi.org/10.1093/jxb/erp138
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author Hofmann, Julia
Hess, Paul H.
Szakasits, Dagmar
Blöchl, Andreas
Wieczorek, Krzysztof
Daxböck-Horvath, Sabine
Bohlmann, Holger
van Bel, Aart J. E.
Grundler, Florian M. W.
author_facet Hofmann, Julia
Hess, Paul H.
Szakasits, Dagmar
Blöchl, Andreas
Wieczorek, Krzysztof
Daxböck-Horvath, Sabine
Bohlmann, Holger
van Bel, Aart J. E.
Grundler, Florian M. W.
author_sort Hofmann, Julia
collection PubMed
description The plant-parasitic nematode Heterodera schachtii stimulates plant root cells to form syncytial feeding structures which synthesize all nutrients required for successful nematode development. Cellular re-arrangements and modified metabolism of the syncytia are accompanied by massive intra- and intercellular solute allocations. In this study the expression of all genes annotated as sugar transporters in the Arabidopsis Membrane Protein Library was investigated by Affymetrix gene chip analysis in young and fully developed syncytia compared with non-infected Arabidopsis thaliana roots. The expression of three highly up-regulated (STP12, MEX1, and GTP2) and three highly down-regulated genes (SFP1, STP7, and STP4) was analysed by quantitative RT-PCR (qRT-PCR). The most up-regulated gene (STP12) was chosen for further in-depth studies using in situ RT-PCR and a nematode development assay with a T-DNA insertion line revealing a significant reduction of male nematode development. The specific role of STP12 expression in syncytia of male juveniles compared with those of female juveniles was further shown by qRT-PCR. In order to provide evidence for sugar transporter activity across the plasma membrane of syncytia, fluorescence-labelled glucose was used and membrane potential recordings following the application of several sugars were performed. Analyses of soluble sugar pools revealed a highly specific composition in syncytia. The presented work demonstrates that sugar transporters are specifically expressed and active in syncytia, indicating a profound role in inter- and intracelluar transport processes.
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spelling pubmed-27182142009-07-31 Diversity and activity of sugar transporters in nematode-induced root syncytia Hofmann, Julia Hess, Paul H. Szakasits, Dagmar Blöchl, Andreas Wieczorek, Krzysztof Daxböck-Horvath, Sabine Bohlmann, Holger van Bel, Aart J. E. Grundler, Florian M. W. J Exp Bot Research Papers The plant-parasitic nematode Heterodera schachtii stimulates plant root cells to form syncytial feeding structures which synthesize all nutrients required for successful nematode development. Cellular re-arrangements and modified metabolism of the syncytia are accompanied by massive intra- and intercellular solute allocations. In this study the expression of all genes annotated as sugar transporters in the Arabidopsis Membrane Protein Library was investigated by Affymetrix gene chip analysis in young and fully developed syncytia compared with non-infected Arabidopsis thaliana roots. The expression of three highly up-regulated (STP12, MEX1, and GTP2) and three highly down-regulated genes (SFP1, STP7, and STP4) was analysed by quantitative RT-PCR (qRT-PCR). The most up-regulated gene (STP12) was chosen for further in-depth studies using in situ RT-PCR and a nematode development assay with a T-DNA insertion line revealing a significant reduction of male nematode development. The specific role of STP12 expression in syncytia of male juveniles compared with those of female juveniles was further shown by qRT-PCR. In order to provide evidence for sugar transporter activity across the plasma membrane of syncytia, fluorescence-labelled glucose was used and membrane potential recordings following the application of several sugars were performed. Analyses of soluble sugar pools revealed a highly specific composition in syncytia. The presented work demonstrates that sugar transporters are specifically expressed and active in syncytia, indicating a profound role in inter- and intracelluar transport processes. Oxford University Press 2009-07 2009-06-01 /pmc/articles/PMC2718214/ /pubmed/19487386 http://dx.doi.org/10.1093/jxb/erp138 Text en © 2009 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)
spellingShingle Research Papers
Hofmann, Julia
Hess, Paul H.
Szakasits, Dagmar
Blöchl, Andreas
Wieczorek, Krzysztof
Daxböck-Horvath, Sabine
Bohlmann, Holger
van Bel, Aart J. E.
Grundler, Florian M. W.
Diversity and activity of sugar transporters in nematode-induced root syncytia
title Diversity and activity of sugar transporters in nematode-induced root syncytia
title_full Diversity and activity of sugar transporters in nematode-induced root syncytia
title_fullStr Diversity and activity of sugar transporters in nematode-induced root syncytia
title_full_unstemmed Diversity and activity of sugar transporters in nematode-induced root syncytia
title_short Diversity and activity of sugar transporters in nematode-induced root syncytia
title_sort diversity and activity of sugar transporters in nematode-induced root syncytia
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2718214/
https://www.ncbi.nlm.nih.gov/pubmed/19487386
http://dx.doi.org/10.1093/jxb/erp138
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