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Virtual Terminator nucleotides for next generation DNA sequencing
We synthesized reversible terminators with tethered inhibitors for next generation sequencing. These were efficiently incorporated with high fidelity while preventing incorporation of additional nucleotides and were used to sequence canine bacterial artificial chromosomes in a single-molecule system...
| Autores principales: | , , , , , , , , , , , , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
2009
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2719685/ https://www.ncbi.nlm.nih.gov/pubmed/19620973 http://dx.doi.org/10.1038/nmeth.1354 |
| _version_ | 1782170091888050176 |
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| author | Bowers, Jayson Mitchell, Judith Beer, Eric Buzby, Philip R. Causey, Marie Efcavitch, J. William Jarosz, Mirna Krzymanska-Olejnik, Edyta Kung, Li Lipson, Doron Lowman, Geoffrey M. Marappan, Subramanian McInerney, Peter Platt, Adam Roy, Atanu Siddiqi, Suhaib M. Steinmann, Kathleen Thompson, John F. |
| author_facet | Bowers, Jayson Mitchell, Judith Beer, Eric Buzby, Philip R. Causey, Marie Efcavitch, J. William Jarosz, Mirna Krzymanska-Olejnik, Edyta Kung, Li Lipson, Doron Lowman, Geoffrey M. Marappan, Subramanian McInerney, Peter Platt, Adam Roy, Atanu Siddiqi, Suhaib M. Steinmann, Kathleen Thompson, John F. |
| author_sort | Bowers, Jayson |
| collection | PubMed |
| description | We synthesized reversible terminators with tethered inhibitors for next generation sequencing. These were efficiently incorporated with high fidelity while preventing incorporation of additional nucleotides and were used to sequence canine bacterial artificial chromosomes in a single-molecule system that provided even coverage for over 99% of the region sequenced. This single-molecule approach generated high quality sequence data without the need for target amplification and thus avoided concomitant biases. |
| format | Text |
| id | pubmed-2719685 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2009 |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-27196852010-02-01 Virtual Terminator nucleotides for next generation DNA sequencing Bowers, Jayson Mitchell, Judith Beer, Eric Buzby, Philip R. Causey, Marie Efcavitch, J. William Jarosz, Mirna Krzymanska-Olejnik, Edyta Kung, Li Lipson, Doron Lowman, Geoffrey M. Marappan, Subramanian McInerney, Peter Platt, Adam Roy, Atanu Siddiqi, Suhaib M. Steinmann, Kathleen Thompson, John F. Nat Methods Article We synthesized reversible terminators with tethered inhibitors for next generation sequencing. These were efficiently incorporated with high fidelity while preventing incorporation of additional nucleotides and were used to sequence canine bacterial artificial chromosomes in a single-molecule system that provided even coverage for over 99% of the region sequenced. This single-molecule approach generated high quality sequence data without the need for target amplification and thus avoided concomitant biases. 2009-07-20 2009-08 /pmc/articles/PMC2719685/ /pubmed/19620973 http://dx.doi.org/10.1038/nmeth.1354 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
| spellingShingle | Article Bowers, Jayson Mitchell, Judith Beer, Eric Buzby, Philip R. Causey, Marie Efcavitch, J. William Jarosz, Mirna Krzymanska-Olejnik, Edyta Kung, Li Lipson, Doron Lowman, Geoffrey M. Marappan, Subramanian McInerney, Peter Platt, Adam Roy, Atanu Siddiqi, Suhaib M. Steinmann, Kathleen Thompson, John F. Virtual Terminator nucleotides for next generation DNA sequencing |
| title | Virtual Terminator nucleotides for next generation DNA sequencing |
| title_full | Virtual Terminator nucleotides for next generation DNA sequencing |
| title_fullStr | Virtual Terminator nucleotides for next generation DNA sequencing |
| title_full_unstemmed | Virtual Terminator nucleotides for next generation DNA sequencing |
| title_short | Virtual Terminator nucleotides for next generation DNA sequencing |
| title_sort | virtual terminator nucleotides for next generation dna sequencing |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2719685/ https://www.ncbi.nlm.nih.gov/pubmed/19620973 http://dx.doi.org/10.1038/nmeth.1354 |
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