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Hepatitis C Virus Infection in Phenotypically Distinct Huh7 Cell Lines

In 2005, the first robust hepatitis C virus (HCV) infectious cell culture system was developed based on the HCV genotype 2a JFH-1 molecular clone and the human-derived hepatoma cell line Huh7. Although much effort has been made to dissect and expand the repertoire of JFH-1-derived clones, less atten...

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Autores principales: Sainz, Bruno, Barretto, Naina, Uprichard, Susan L.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2720605/
https://www.ncbi.nlm.nih.gov/pubmed/19668344
http://dx.doi.org/10.1371/journal.pone.0006561
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author Sainz, Bruno
Barretto, Naina
Uprichard, Susan L.
author_facet Sainz, Bruno
Barretto, Naina
Uprichard, Susan L.
author_sort Sainz, Bruno
collection PubMed
description In 2005, the first robust hepatitis C virus (HCV) infectious cell culture system was developed based on the HCV genotype 2a JFH-1 molecular clone and the human-derived hepatoma cell line Huh7. Although much effort has been made to dissect and expand the repertoire of JFH-1-derived clones, less attention has been given to the host cell despite the intriguing facts that thus far only Huh7 cells have been found to be highly permissive for HCV infection and furthermore only a limited number of Huh7 cell lines/stocks appear to be fully permissive. As such, we compiled a panel of Huh7 lines from disparate sources and evaluated their permissiveness for HCV infection. We found that although Huh7 lines from different laboratories do vary in morphology and cell growth, the majority (8 out of 9) were highly permissive for infection, as demonstrated by robust HCV RNA and de novo infectious virion production following infection. While HCV RNA levels achieved in the 8 permissive cell lines were relatively equivalent, three Huh7 lines demonstrated higher infectious virion production suggesting these cell lines more efficiently support post-replication event(s) in the viral life cycle. Consistent with previous studies, the single Huh7 line found to be relatively resistant to infection demonstrated a block in HCV entry. These studies not only suggest that the majority of Huh7 cell lines in different laboratories are in fact highly permissive for HCV infection, but also identify phenotypically distinct Huh7 lines, which may facilitate studies investigating the cellular determinants of HCV infection.
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spelling pubmed-27206052009-08-10 Hepatitis C Virus Infection in Phenotypically Distinct Huh7 Cell Lines Sainz, Bruno Barretto, Naina Uprichard, Susan L. PLoS One Research Article In 2005, the first robust hepatitis C virus (HCV) infectious cell culture system was developed based on the HCV genotype 2a JFH-1 molecular clone and the human-derived hepatoma cell line Huh7. Although much effort has been made to dissect and expand the repertoire of JFH-1-derived clones, less attention has been given to the host cell despite the intriguing facts that thus far only Huh7 cells have been found to be highly permissive for HCV infection and furthermore only a limited number of Huh7 cell lines/stocks appear to be fully permissive. As such, we compiled a panel of Huh7 lines from disparate sources and evaluated their permissiveness for HCV infection. We found that although Huh7 lines from different laboratories do vary in morphology and cell growth, the majority (8 out of 9) were highly permissive for infection, as demonstrated by robust HCV RNA and de novo infectious virion production following infection. While HCV RNA levels achieved in the 8 permissive cell lines were relatively equivalent, three Huh7 lines demonstrated higher infectious virion production suggesting these cell lines more efficiently support post-replication event(s) in the viral life cycle. Consistent with previous studies, the single Huh7 line found to be relatively resistant to infection demonstrated a block in HCV entry. These studies not only suggest that the majority of Huh7 cell lines in different laboratories are in fact highly permissive for HCV infection, but also identify phenotypically distinct Huh7 lines, which may facilitate studies investigating the cellular determinants of HCV infection. Public Library of Science 2009-08-10 /pmc/articles/PMC2720605/ /pubmed/19668344 http://dx.doi.org/10.1371/journal.pone.0006561 Text en Sainz, Jr., et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Sainz, Bruno
Barretto, Naina
Uprichard, Susan L.
Hepatitis C Virus Infection in Phenotypically Distinct Huh7 Cell Lines
title Hepatitis C Virus Infection in Phenotypically Distinct Huh7 Cell Lines
title_full Hepatitis C Virus Infection in Phenotypically Distinct Huh7 Cell Lines
title_fullStr Hepatitis C Virus Infection in Phenotypically Distinct Huh7 Cell Lines
title_full_unstemmed Hepatitis C Virus Infection in Phenotypically Distinct Huh7 Cell Lines
title_short Hepatitis C Virus Infection in Phenotypically Distinct Huh7 Cell Lines
title_sort hepatitis c virus infection in phenotypically distinct huh7 cell lines
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2720605/
https://www.ncbi.nlm.nih.gov/pubmed/19668344
http://dx.doi.org/10.1371/journal.pone.0006561
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