The Twin Spot Generator for differential Drosophila lineage analysis

In Drosophila, widely-used mitotic recombination-based strategies generate mosaic flies with positive readout for only one daughter cell after division. To differentially label both daughter cells, we developed the Twin Spot Generator technique (TSG) and demonstrate that through mitotic recombinatio...

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Autores principales: Griffin, Ruth, Sustar, Anne, Bonvin, Marianne, Binari, Richard, del Valle Rodriguez, Alberto, Bakal, Chris, Hohl, Amber M., Bateman, Jack R., Villalta, Christians, Heffern, Elleard, Grunwald, Didier, Desplan, Claude, Schubiger, Gerold, Wu, C.-ting, Perrimon, Norbert
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2720837/
https://www.ncbi.nlm.nih.gov/pubmed/19633664
http://dx.doi.org/10.1038/nmeth.1349
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author Griffin, Ruth
Sustar, Anne
Bonvin, Marianne
Binari, Richard
del Valle Rodriguez, Alberto
Bakal, Chris
Hohl, Amber M.
Bateman, Jack R.
Villalta, Christians
Heffern, Elleard
Grunwald, Didier
Desplan, Claude
Schubiger, Gerold
Wu, C.-ting
Perrimon, Norbert
author_facet Griffin, Ruth
Sustar, Anne
Bonvin, Marianne
Binari, Richard
del Valle Rodriguez, Alberto
Bakal, Chris
Hohl, Amber M.
Bateman, Jack R.
Villalta, Christians
Heffern, Elleard
Grunwald, Didier
Desplan, Claude
Schubiger, Gerold
Wu, C.-ting
Perrimon, Norbert
author_sort Griffin, Ruth
collection PubMed
description In Drosophila, widely-used mitotic recombination-based strategies generate mosaic flies with positive readout for only one daughter cell after division. To differentially label both daughter cells, we developed the Twin Spot Generator technique (TSG) and demonstrate that through mitotic recombination, TSG generates green and red twin spots in internal fly tissues, visible even as single cells. We discuss the wide applications of TSG to lineage and genetic mosaic studies.
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spelling pubmed-27208372010-02-01 The Twin Spot Generator for differential Drosophila lineage analysis Griffin, Ruth Sustar, Anne Bonvin, Marianne Binari, Richard del Valle Rodriguez, Alberto Bakal, Chris Hohl, Amber M. Bateman, Jack R. Villalta, Christians Heffern, Elleard Grunwald, Didier Desplan, Claude Schubiger, Gerold Wu, C.-ting Perrimon, Norbert Nat Methods Article In Drosophila, widely-used mitotic recombination-based strategies generate mosaic flies with positive readout for only one daughter cell after division. To differentially label both daughter cells, we developed the Twin Spot Generator technique (TSG) and demonstrate that through mitotic recombination, TSG generates green and red twin spots in internal fly tissues, visible even as single cells. We discuss the wide applications of TSG to lineage and genetic mosaic studies. 2009-07-26 2009-08 /pmc/articles/PMC2720837/ /pubmed/19633664 http://dx.doi.org/10.1038/nmeth.1349 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Griffin, Ruth
Sustar, Anne
Bonvin, Marianne
Binari, Richard
del Valle Rodriguez, Alberto
Bakal, Chris
Hohl, Amber M.
Bateman, Jack R.
Villalta, Christians
Heffern, Elleard
Grunwald, Didier
Desplan, Claude
Schubiger, Gerold
Wu, C.-ting
Perrimon, Norbert
The Twin Spot Generator for differential Drosophila lineage analysis
title The Twin Spot Generator for differential Drosophila lineage analysis
title_full The Twin Spot Generator for differential Drosophila lineage analysis
title_fullStr The Twin Spot Generator for differential Drosophila lineage analysis
title_full_unstemmed The Twin Spot Generator for differential Drosophila lineage analysis
title_short The Twin Spot Generator for differential Drosophila lineage analysis
title_sort twin spot generator for differential drosophila lineage analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2720837/
https://www.ncbi.nlm.nih.gov/pubmed/19633664
http://dx.doi.org/10.1038/nmeth.1349
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