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Carbon and arsenic metabolism in Thiomonas strains: differences revealed diverse adaptation processes

BACKGROUND: Thiomonas strains are ubiquitous in arsenic-contaminated environments. Differences between Thiomonas strains in the way they have adapted and respond to arsenic have never been studied in detail. For this purpose, five Thiomonas strains, that are interesting in terms of arsenic metabolis...

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Autores principales: Bryan, Christopher G, Marchal, Marie, Battaglia-Brunet, Fabienne, Kugler, Valérie, Lemaitre-Guillier, Christelle, Lièvremont, Didier, Bertin, Philippe N, Arsène-Ploetze, Florence
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2720973/
https://www.ncbi.nlm.nih.gov/pubmed/19549320
http://dx.doi.org/10.1186/1471-2180-9-127
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author Bryan, Christopher G
Marchal, Marie
Battaglia-Brunet, Fabienne
Kugler, Valérie
Lemaitre-Guillier, Christelle
Lièvremont, Didier
Bertin, Philippe N
Arsène-Ploetze, Florence
author_facet Bryan, Christopher G
Marchal, Marie
Battaglia-Brunet, Fabienne
Kugler, Valérie
Lemaitre-Guillier, Christelle
Lièvremont, Didier
Bertin, Philippe N
Arsène-Ploetze, Florence
author_sort Bryan, Christopher G
collection PubMed
description BACKGROUND: Thiomonas strains are ubiquitous in arsenic-contaminated environments. Differences between Thiomonas strains in the way they have adapted and respond to arsenic have never been studied in detail. For this purpose, five Thiomonas strains, that are interesting in terms of arsenic metabolism were selected: T. arsenivorans, Thiomonas spp. WJ68 and 3As are able to oxidise As(III), while Thiomonas sp. Ynys1 and T. perometabolis are not. Moreover, T. arsenivorans and 3As present interesting physiological traits, in particular that these strains are able to use As(III) as an electron donor. RESULTS: The metabolism of carbon and arsenic was compared in the five Thiomonas strains belonging to two distinct phylogenetic groups. Greater physiological differences were found between these strains than might have been suggested by 16S rRNA/rpoA gene phylogeny, especially regarding arsenic metabolism. Physiologically, T. perometabolis and Ynys1 were unable to oxidise As(III) and were less arsenic-resistant than the other strains. Genetically, they appeared to lack the aox arsenic-oxidising genes and carried only a single ars arsenic resistance operon. Thiomonas arsenivorans belonged to a distinct phylogenetic group and increased its autotrophic metabolism when arsenic concentration increased. Differential proteomic analysis revealed that in T. arsenivorans, the rbc/cbb genes involved in the assimilation of inorganic carbon were induced in the presence of arsenic, whereas these genes were repressed in Thiomonas sp. 3As. CONCLUSION: Taken together, these results show that these closely related bacteria differ substantially in their response to arsenic, amongst other factors, and suggest different relationships between carbon assimilation and arsenic metabolism.
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spelling pubmed-27209732009-08-05 Carbon and arsenic metabolism in Thiomonas strains: differences revealed diverse adaptation processes Bryan, Christopher G Marchal, Marie Battaglia-Brunet, Fabienne Kugler, Valérie Lemaitre-Guillier, Christelle Lièvremont, Didier Bertin, Philippe N Arsène-Ploetze, Florence BMC Microbiol Research article BACKGROUND: Thiomonas strains are ubiquitous in arsenic-contaminated environments. Differences between Thiomonas strains in the way they have adapted and respond to arsenic have never been studied in detail. For this purpose, five Thiomonas strains, that are interesting in terms of arsenic metabolism were selected: T. arsenivorans, Thiomonas spp. WJ68 and 3As are able to oxidise As(III), while Thiomonas sp. Ynys1 and T. perometabolis are not. Moreover, T. arsenivorans and 3As present interesting physiological traits, in particular that these strains are able to use As(III) as an electron donor. RESULTS: The metabolism of carbon and arsenic was compared in the five Thiomonas strains belonging to two distinct phylogenetic groups. Greater physiological differences were found between these strains than might have been suggested by 16S rRNA/rpoA gene phylogeny, especially regarding arsenic metabolism. Physiologically, T. perometabolis and Ynys1 were unable to oxidise As(III) and were less arsenic-resistant than the other strains. Genetically, they appeared to lack the aox arsenic-oxidising genes and carried only a single ars arsenic resistance operon. Thiomonas arsenivorans belonged to a distinct phylogenetic group and increased its autotrophic metabolism when arsenic concentration increased. Differential proteomic analysis revealed that in T. arsenivorans, the rbc/cbb genes involved in the assimilation of inorganic carbon were induced in the presence of arsenic, whereas these genes were repressed in Thiomonas sp. 3As. CONCLUSION: Taken together, these results show that these closely related bacteria differ substantially in their response to arsenic, amongst other factors, and suggest different relationships between carbon assimilation and arsenic metabolism. BioMed Central 2009-06-23 /pmc/articles/PMC2720973/ /pubmed/19549320 http://dx.doi.org/10.1186/1471-2180-9-127 Text en Copyright ©2009 Bryan et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research article
Bryan, Christopher G
Marchal, Marie
Battaglia-Brunet, Fabienne
Kugler, Valérie
Lemaitre-Guillier, Christelle
Lièvremont, Didier
Bertin, Philippe N
Arsène-Ploetze, Florence
Carbon and arsenic metabolism in Thiomonas strains: differences revealed diverse adaptation processes
title Carbon and arsenic metabolism in Thiomonas strains: differences revealed diverse adaptation processes
title_full Carbon and arsenic metabolism in Thiomonas strains: differences revealed diverse adaptation processes
title_fullStr Carbon and arsenic metabolism in Thiomonas strains: differences revealed diverse adaptation processes
title_full_unstemmed Carbon and arsenic metabolism in Thiomonas strains: differences revealed diverse adaptation processes
title_short Carbon and arsenic metabolism in Thiomonas strains: differences revealed diverse adaptation processes
title_sort carbon and arsenic metabolism in thiomonas strains: differences revealed diverse adaptation processes
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2720973/
https://www.ncbi.nlm.nih.gov/pubmed/19549320
http://dx.doi.org/10.1186/1471-2180-9-127
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