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Pretreatment with N-nitro-L-arginine Methyl Ester Improved Oxygenation After Inhalation of Nitric Oxide in Newborn Piglets with Escherichia coli Pneumonia and Sepsis

We evaluated the effects of a combined therapy of pre-blockade endogenous nitric oxide synthase (NOS) with N-nitro-L-arginine methyl ester (L-NAME) and continuous inhaled NO (iNO) on the gas exchange and hemodynamics of Escherichia coli pneumonia and sepsis in newborn piglets. Seven to ten day old v...

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Detalles Bibliográficos
Autores principales: Chang, Yun Sil, Kang, Saem, Ko, Sun Young, Park, Won Soon
Formato: Texto
Lenguaje:English
Publicado: The Korean Academy of Medical Sciences 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2721948/
https://www.ncbi.nlm.nih.gov/pubmed/17179670
http://dx.doi.org/10.3346/jkms.2006.21.6.965
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author Chang, Yun Sil
Kang, Saem
Ko, Sun Young
Park, Won Soon
author_facet Chang, Yun Sil
Kang, Saem
Ko, Sun Young
Park, Won Soon
author_sort Chang, Yun Sil
collection PubMed
description We evaluated the effects of a combined therapy of pre-blockade endogenous nitric oxide synthase (NOS) with N-nitro-L-arginine methyl ester (L-NAME) and continuous inhaled NO (iNO) on the gas exchange and hemodynamics of Escherichia coli pneumonia and sepsis in newborn piglets. Seven to ten day old ventilated newborn piglets were randomized into 5 groups: control, E. coli pneumonia control, pneumonia with iNO 10 ppm, pneumonia pre-treated with L-NAME 10 mg/kg, and pneumonia with the combined therapy of L-NAME pretreatment and iNO. E. coli pneumonia was induced via intratracheal instillation of Escherichia coli, which resulted in progressively decreased cardiac index and oxygen tension; increased pulmonary vascular resistance index (PVRI), intrapulmonary shunting, and developed septicemia at the end of 6 hr experiment. iNO ameliorated the progressive hypoxemia and intrapulmonary shunting without affecting the PVRI. Only two of 8 animals with L-NAME-pretreated pneumonia survived. Whereas when iNO was added to infected animals with L-NAME pretreatment, the progressive hypoxemia was abolished as a result of a decrease in intrapulmonary shunting without reverse of the high PVRI and systemic vascular resistance index induced by the L-NAME injection. This result suggests that a NOS blockade may be a possible supportive option for oxygenation by iNO treatment in neonatal Gram-negative bacterial pneumonia and sepsis.
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spelling pubmed-27219482009-08-07 Pretreatment with N-nitro-L-arginine Methyl Ester Improved Oxygenation After Inhalation of Nitric Oxide in Newborn Piglets with Escherichia coli Pneumonia and Sepsis Chang, Yun Sil Kang, Saem Ko, Sun Young Park, Won Soon J Korean Med Sci Original Article We evaluated the effects of a combined therapy of pre-blockade endogenous nitric oxide synthase (NOS) with N-nitro-L-arginine methyl ester (L-NAME) and continuous inhaled NO (iNO) on the gas exchange and hemodynamics of Escherichia coli pneumonia and sepsis in newborn piglets. Seven to ten day old ventilated newborn piglets were randomized into 5 groups: control, E. coli pneumonia control, pneumonia with iNO 10 ppm, pneumonia pre-treated with L-NAME 10 mg/kg, and pneumonia with the combined therapy of L-NAME pretreatment and iNO. E. coli pneumonia was induced via intratracheal instillation of Escherichia coli, which resulted in progressively decreased cardiac index and oxygen tension; increased pulmonary vascular resistance index (PVRI), intrapulmonary shunting, and developed septicemia at the end of 6 hr experiment. iNO ameliorated the progressive hypoxemia and intrapulmonary shunting without affecting the PVRI. Only two of 8 animals with L-NAME-pretreated pneumonia survived. Whereas when iNO was added to infected animals with L-NAME pretreatment, the progressive hypoxemia was abolished as a result of a decrease in intrapulmonary shunting without reverse of the high PVRI and systemic vascular resistance index induced by the L-NAME injection. This result suggests that a NOS blockade may be a possible supportive option for oxygenation by iNO treatment in neonatal Gram-negative bacterial pneumonia and sepsis. The Korean Academy of Medical Sciences 2006-12 2006-12-31 /pmc/articles/PMC2721948/ /pubmed/17179670 http://dx.doi.org/10.3346/jkms.2006.21.6.965 Text en Copyright © 2006 The Korean Academy of Medical Sciences http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Chang, Yun Sil
Kang, Saem
Ko, Sun Young
Park, Won Soon
Pretreatment with N-nitro-L-arginine Methyl Ester Improved Oxygenation After Inhalation of Nitric Oxide in Newborn Piglets with Escherichia coli Pneumonia and Sepsis
title Pretreatment with N-nitro-L-arginine Methyl Ester Improved Oxygenation After Inhalation of Nitric Oxide in Newborn Piglets with Escherichia coli Pneumonia and Sepsis
title_full Pretreatment with N-nitro-L-arginine Methyl Ester Improved Oxygenation After Inhalation of Nitric Oxide in Newborn Piglets with Escherichia coli Pneumonia and Sepsis
title_fullStr Pretreatment with N-nitro-L-arginine Methyl Ester Improved Oxygenation After Inhalation of Nitric Oxide in Newborn Piglets with Escherichia coli Pneumonia and Sepsis
title_full_unstemmed Pretreatment with N-nitro-L-arginine Methyl Ester Improved Oxygenation After Inhalation of Nitric Oxide in Newborn Piglets with Escherichia coli Pneumonia and Sepsis
title_short Pretreatment with N-nitro-L-arginine Methyl Ester Improved Oxygenation After Inhalation of Nitric Oxide in Newborn Piglets with Escherichia coli Pneumonia and Sepsis
title_sort pretreatment with n-nitro-l-arginine methyl ester improved oxygenation after inhalation of nitric oxide in newborn piglets with escherichia coli pneumonia and sepsis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2721948/
https://www.ncbi.nlm.nih.gov/pubmed/17179670
http://dx.doi.org/10.3346/jkms.2006.21.6.965
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