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Merkel Cells as Putative Regulatory Cells in Skin Disorders: An In Vitro Study
Merkel cells (MCs) are involved in mechanoreception, but several lines of evidence suggest that they may also participate in skin disorders through the release of neuropeptides and hormones. In addition, MC hyperplasias have been reported in inflammatory skin diseases. However, neither proliferation...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2722079/ https://www.ncbi.nlm.nih.gov/pubmed/19668696 http://dx.doi.org/10.1371/journal.pone.0006528 |
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author | Boulais, Nicholas Pereira, Ulysse Lebonvallet, Nicolas Gobin, Eric Dorange, Germaine Rougier, Nathalie Chesne, Christophe Misery, Laurent |
author_facet | Boulais, Nicholas Pereira, Ulysse Lebonvallet, Nicolas Gobin, Eric Dorange, Germaine Rougier, Nathalie Chesne, Christophe Misery, Laurent |
author_sort | Boulais, Nicholas |
collection | PubMed |
description | Merkel cells (MCs) are involved in mechanoreception, but several lines of evidence suggest that they may also participate in skin disorders through the release of neuropeptides and hormones. In addition, MC hyperplasias have been reported in inflammatory skin diseases. However, neither proliferation nor reactions to the epidermal environment have been demonstrated. We established a culture model enriched in swine MCs to analyze their proliferative capability and to discover MC survival factors and modulators of MC neuroendocrine properties. In culture, MCs reacted to bFGF by extending outgrowths. Conversely, neurotrophins failed to induce cell spreading, suggesting that they do not act as a growth factor for MCs. For the first time, we provide evidence of proliferation in culture through Ki-67 immunoreactivity. We also found that MCs reacted to histamine or activation of the proton gated/osmoreceptor TRPV4 by releasing vasoactive intestinal peptide (VIP). Since VIP is involved in many pathophysiological processes, its release suggests a putative regulatory role for MCs in skin disorders. Moreover, in contrast to mechanotransduction, neuropeptide exocytosis was Ca(2+)-independent, as inhibition of Ca(2+) channels or culture in the absence of Ca(2+) failed to decrease the amount of VIP released. We conclude that neuropeptide release and neurotransmitter exocytosis may be two distinct pathways that are differentially regulated. |
format | Text |
id | pubmed-2722079 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-27220792009-08-11 Merkel Cells as Putative Regulatory Cells in Skin Disorders: An In Vitro Study Boulais, Nicholas Pereira, Ulysse Lebonvallet, Nicolas Gobin, Eric Dorange, Germaine Rougier, Nathalie Chesne, Christophe Misery, Laurent PLoS One Research Article Merkel cells (MCs) are involved in mechanoreception, but several lines of evidence suggest that they may also participate in skin disorders through the release of neuropeptides and hormones. In addition, MC hyperplasias have been reported in inflammatory skin diseases. However, neither proliferation nor reactions to the epidermal environment have been demonstrated. We established a culture model enriched in swine MCs to analyze their proliferative capability and to discover MC survival factors and modulators of MC neuroendocrine properties. In culture, MCs reacted to bFGF by extending outgrowths. Conversely, neurotrophins failed to induce cell spreading, suggesting that they do not act as a growth factor for MCs. For the first time, we provide evidence of proliferation in culture through Ki-67 immunoreactivity. We also found that MCs reacted to histamine or activation of the proton gated/osmoreceptor TRPV4 by releasing vasoactive intestinal peptide (VIP). Since VIP is involved in many pathophysiological processes, its release suggests a putative regulatory role for MCs in skin disorders. Moreover, in contrast to mechanotransduction, neuropeptide exocytosis was Ca(2+)-independent, as inhibition of Ca(2+) channels or culture in the absence of Ca(2+) failed to decrease the amount of VIP released. We conclude that neuropeptide release and neurotransmitter exocytosis may be two distinct pathways that are differentially regulated. Public Library of Science 2009-08-11 /pmc/articles/PMC2722079/ /pubmed/19668696 http://dx.doi.org/10.1371/journal.pone.0006528 Text en Boulais et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Boulais, Nicholas Pereira, Ulysse Lebonvallet, Nicolas Gobin, Eric Dorange, Germaine Rougier, Nathalie Chesne, Christophe Misery, Laurent Merkel Cells as Putative Regulatory Cells in Skin Disorders: An In Vitro Study |
title | Merkel Cells as Putative Regulatory Cells in Skin Disorders: An In Vitro Study |
title_full | Merkel Cells as Putative Regulatory Cells in Skin Disorders: An In Vitro Study |
title_fullStr | Merkel Cells as Putative Regulatory Cells in Skin Disorders: An In Vitro Study |
title_full_unstemmed | Merkel Cells as Putative Regulatory Cells in Skin Disorders: An In Vitro Study |
title_short | Merkel Cells as Putative Regulatory Cells in Skin Disorders: An In Vitro Study |
title_sort | merkel cells as putative regulatory cells in skin disorders: an in vitro study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2722079/ https://www.ncbi.nlm.nih.gov/pubmed/19668696 http://dx.doi.org/10.1371/journal.pone.0006528 |
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