Cargando…
Crocus Sativus L. (Saffron) Extract and its Active Constituents (Crocin and Safranal) on Ischemia-Reperfusion in Rat Skeletal Muscle
Saffron and its constituents have been shown to decrease ischemia-reperfusion (I/R) injury in kidney or brain tissues. In this study, the effects of saffron ethanolic extract and its constituents, crocin and safranal, were evaluated in skeletal muscle during I/R injury. Hind limb ischemia was induce...
Autores principales: | , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2722204/ https://www.ncbi.nlm.nih.gov/pubmed/18955256 http://dx.doi.org/10.1093/ecam/nem125 |
_version_ | 1782170296485150720 |
---|---|
author | Hosseinzadeh, Hossein Modaghegh, Mohammad Hadi Saffari, Zahra |
author_facet | Hosseinzadeh, Hossein Modaghegh, Mohammad Hadi Saffari, Zahra |
author_sort | Hosseinzadeh, Hossein |
collection | PubMed |
description | Saffron and its constituents have been shown to decrease ischemia-reperfusion (I/R) injury in kidney or brain tissues. In this study, the effects of saffron ethanolic extract and its constituents, crocin and safranal, were evaluated in skeletal muscle during I/R injury. Hind limb ischemia was induced using clamping the common femoral artery and vein. After 2 h ischemia, the clamp of the femoral vessels of animals was taken off and the animal underwent 1h reperfusion. Muscle injuries were evaluated by recording of the electromyographic (EMG) potentials and performing some biochemical analysis including thiobarbituric acid reactive substances (TBARS), total sulfhydryl (SH) groups and antioxidant capacity of muscle (using FRAP assay). The ethanolic extract of saffron (5, 20 and 80 mg kg(−1)), crocin (50, 200 and 400 mg kg(−1)), safranal (0.1, 0.25 and 0.5 ml kg(−1)) and normal saline (10 ml kg(−1)) were administered intraperitoneally 1 h prior reperfusion. The average peak-to-peak amplitude during I/R was significantly increased in extract, crocin and safranal groups in comparison with control-ischemic group. Following saffron, crocin and safranal administration, the total SH contents and antioxidant capacity were elevated in muscle flap. The MDA level was declined significantly in test groups. It is concluded that saffron extract and its constituents show a protective effect against lower limb I/R in rat. |
format | Text |
id | pubmed-2722204 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-27222042009-08-06 Crocus Sativus L. (Saffron) Extract and its Active Constituents (Crocin and Safranal) on Ischemia-Reperfusion in Rat Skeletal Muscle Hosseinzadeh, Hossein Modaghegh, Mohammad Hadi Saffari, Zahra Evid Based Complement Alternat Med Original Articles - Basic Science Saffron and its constituents have been shown to decrease ischemia-reperfusion (I/R) injury in kidney or brain tissues. In this study, the effects of saffron ethanolic extract and its constituents, crocin and safranal, were evaluated in skeletal muscle during I/R injury. Hind limb ischemia was induced using clamping the common femoral artery and vein. After 2 h ischemia, the clamp of the femoral vessels of animals was taken off and the animal underwent 1h reperfusion. Muscle injuries were evaluated by recording of the electromyographic (EMG) potentials and performing some biochemical analysis including thiobarbituric acid reactive substances (TBARS), total sulfhydryl (SH) groups and antioxidant capacity of muscle (using FRAP assay). The ethanolic extract of saffron (5, 20 and 80 mg kg(−1)), crocin (50, 200 and 400 mg kg(−1)), safranal (0.1, 0.25 and 0.5 ml kg(−1)) and normal saline (10 ml kg(−1)) were administered intraperitoneally 1 h prior reperfusion. The average peak-to-peak amplitude during I/R was significantly increased in extract, crocin and safranal groups in comparison with control-ischemic group. Following saffron, crocin and safranal administration, the total SH contents and antioxidant capacity were elevated in muscle flap. The MDA level was declined significantly in test groups. It is concluded that saffron extract and its constituents show a protective effect against lower limb I/R in rat. Oxford University Press 2009-09 2007-10-25 /pmc/articles/PMC2722204/ /pubmed/18955256 http://dx.doi.org/10.1093/ecam/nem125 Text en © 2007 The Author(s). http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles - Basic Science Hosseinzadeh, Hossein Modaghegh, Mohammad Hadi Saffari, Zahra Crocus Sativus L. (Saffron) Extract and its Active Constituents (Crocin and Safranal) on Ischemia-Reperfusion in Rat Skeletal Muscle |
title | Crocus Sativus L. (Saffron) Extract and its Active Constituents (Crocin and Safranal) on Ischemia-Reperfusion in Rat Skeletal Muscle |
title_full | Crocus Sativus L. (Saffron) Extract and its Active Constituents (Crocin and Safranal) on Ischemia-Reperfusion in Rat Skeletal Muscle |
title_fullStr | Crocus Sativus L. (Saffron) Extract and its Active Constituents (Crocin and Safranal) on Ischemia-Reperfusion in Rat Skeletal Muscle |
title_full_unstemmed | Crocus Sativus L. (Saffron) Extract and its Active Constituents (Crocin and Safranal) on Ischemia-Reperfusion in Rat Skeletal Muscle |
title_short | Crocus Sativus L. (Saffron) Extract and its Active Constituents (Crocin and Safranal) on Ischemia-Reperfusion in Rat Skeletal Muscle |
title_sort | crocus sativus l. (saffron) extract and its active constituents (crocin and safranal) on ischemia-reperfusion in rat skeletal muscle |
topic | Original Articles - Basic Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2722204/ https://www.ncbi.nlm.nih.gov/pubmed/18955256 http://dx.doi.org/10.1093/ecam/nem125 |
work_keys_str_mv | AT hosseinzadehhossein crocussativuslsaffronextractanditsactiveconstituentscrocinandsafranalonischemiareperfusioninratskeletalmuscle AT modagheghmohammadhadi crocussativuslsaffronextractanditsactiveconstituentscrocinandsafranalonischemiareperfusioninratskeletalmuscle AT saffarizahra crocussativuslsaffronextractanditsactiveconstituentscrocinandsafranalonischemiareperfusioninratskeletalmuscle |