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Production of N-acetyl-D-neuraminic acid using two sequential enzymes overexpressed as double-tagged fusion proteins

BACKGROUND: Two sequential enzymes in the production of sialic acids, N-acetyl-D-glucosamine 2-epimerase (GlcNAc 2-epimerase) and N-acetyl-D-neuraminic acid aldolase (Neu5Ac aldolase), were overexpressed as double-tagged gene fusions. Both were tagged with glutathione S-transferase (GST) at the N-te...

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Detalles Bibliográficos
Autores principales:	Wang, Tzu-Hsien, Chen, Ying-Yin, Pan, Hsin-Hung, Wang, Feng-Pao, Cheng, Chung-Hsien, Lee, Wen-Chien
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2009
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2722590/ https://www.ncbi.nlm.nih.gov/pubmed/19586552 http://dx.doi.org/10.1186/1472-6750-9-63

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