Cargando…

MiR-107 and MiR-185 Can Induce Cell Cycle Arrest in Human Non Small Cell Lung Cancer Cell Lines

BACKGROUND: MicroRNAs (miRNAs) are short single stranded noncoding RNAs that suppress gene expression through either translational repression or degradation of target mRNAs. The annealing between messenger RNAs and 5′ seed region of miRNAs is believed to be essential for the specific suppression of...

Descripción completa

Detalles Bibliográficos
Autores principales: Takahashi, Yukari, Forrest, Alistair R. R., Maeno, Emi, Hashimoto, Takehiro, Daub, Carsten O., Yasuda, Jun
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2722734/
https://www.ncbi.nlm.nih.gov/pubmed/19688090
http://dx.doi.org/10.1371/journal.pone.0006677
_version_ 1782170334859886592
author Takahashi, Yukari
Forrest, Alistair R. R.
Maeno, Emi
Hashimoto, Takehiro
Daub, Carsten O.
Yasuda, Jun
author_facet Takahashi, Yukari
Forrest, Alistair R. R.
Maeno, Emi
Hashimoto, Takehiro
Daub, Carsten O.
Yasuda, Jun
author_sort Takahashi, Yukari
collection PubMed
description BACKGROUND: MicroRNAs (miRNAs) are short single stranded noncoding RNAs that suppress gene expression through either translational repression or degradation of target mRNAs. The annealing between messenger RNAs and 5′ seed region of miRNAs is believed to be essential for the specific suppression of target gene expression. One miRNA can have several hundred different targets in a cell. Rapidly accumulating evidence suggests that many miRNAs are involved in cell cycle regulation and consequentially play critical roles in carcinogenesis. METHODOLOGY/PRINCIPAL FINDINGS: Introduction of synthetic miR-107 or miR-185 suppressed growth of the human non-small cell lung cancer cell lines. Flow cytometry analysis revealed these miRNAs induce a G1 cell cycle arrest in H1299 cells and the suppression of cell cycle progression is stronger than that by Let-7 miRNA. By the gene expression analyses with oligonucleotide microarrays, we find hundreds of genes are affected by transfection of these miRNAs. Using miRNA-target prediction analyses and the array data, we listed up a set of likely targets of miR-107 and miR-185 for G1 cell cycle arrest and validate a subset of them using real-time RT-PCR and immunoblotting for CDK6. CONCLUSIONS/SIGNIFICANCE: We identified new cell cycle regulating miRNAs, miR-107 and miR-185, localized in frequently altered chromosomal regions in human lung cancers. Especially for miR-107, a large number of down-regulated genes are annotated with the gene ontology term ‘cell cycle’. Our results suggest that these miRNAs may contribute to regulate cell cycle in human malignant tumors.
format Text
id pubmed-2722734
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-27227342009-08-18 MiR-107 and MiR-185 Can Induce Cell Cycle Arrest in Human Non Small Cell Lung Cancer Cell Lines Takahashi, Yukari Forrest, Alistair R. R. Maeno, Emi Hashimoto, Takehiro Daub, Carsten O. Yasuda, Jun PLoS One Research Article BACKGROUND: MicroRNAs (miRNAs) are short single stranded noncoding RNAs that suppress gene expression through either translational repression or degradation of target mRNAs. The annealing between messenger RNAs and 5′ seed region of miRNAs is believed to be essential for the specific suppression of target gene expression. One miRNA can have several hundred different targets in a cell. Rapidly accumulating evidence suggests that many miRNAs are involved in cell cycle regulation and consequentially play critical roles in carcinogenesis. METHODOLOGY/PRINCIPAL FINDINGS: Introduction of synthetic miR-107 or miR-185 suppressed growth of the human non-small cell lung cancer cell lines. Flow cytometry analysis revealed these miRNAs induce a G1 cell cycle arrest in H1299 cells and the suppression of cell cycle progression is stronger than that by Let-7 miRNA. By the gene expression analyses with oligonucleotide microarrays, we find hundreds of genes are affected by transfection of these miRNAs. Using miRNA-target prediction analyses and the array data, we listed up a set of likely targets of miR-107 and miR-185 for G1 cell cycle arrest and validate a subset of them using real-time RT-PCR and immunoblotting for CDK6. CONCLUSIONS/SIGNIFICANCE: We identified new cell cycle regulating miRNAs, miR-107 and miR-185, localized in frequently altered chromosomal regions in human lung cancers. Especially for miR-107, a large number of down-regulated genes are annotated with the gene ontology term ‘cell cycle’. Our results suggest that these miRNAs may contribute to regulate cell cycle in human malignant tumors. Public Library of Science 2009-08-18 /pmc/articles/PMC2722734/ /pubmed/19688090 http://dx.doi.org/10.1371/journal.pone.0006677 Text en Takahashi et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Takahashi, Yukari
Forrest, Alistair R. R.
Maeno, Emi
Hashimoto, Takehiro
Daub, Carsten O.
Yasuda, Jun
MiR-107 and MiR-185 Can Induce Cell Cycle Arrest in Human Non Small Cell Lung Cancer Cell Lines
title MiR-107 and MiR-185 Can Induce Cell Cycle Arrest in Human Non Small Cell Lung Cancer Cell Lines
title_full MiR-107 and MiR-185 Can Induce Cell Cycle Arrest in Human Non Small Cell Lung Cancer Cell Lines
title_fullStr MiR-107 and MiR-185 Can Induce Cell Cycle Arrest in Human Non Small Cell Lung Cancer Cell Lines
title_full_unstemmed MiR-107 and MiR-185 Can Induce Cell Cycle Arrest in Human Non Small Cell Lung Cancer Cell Lines
title_short MiR-107 and MiR-185 Can Induce Cell Cycle Arrest in Human Non Small Cell Lung Cancer Cell Lines
title_sort mir-107 and mir-185 can induce cell cycle arrest in human non small cell lung cancer cell lines
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2722734/
https://www.ncbi.nlm.nih.gov/pubmed/19688090
http://dx.doi.org/10.1371/journal.pone.0006677
work_keys_str_mv AT takahashiyukari mir107andmir185caninducecellcyclearrestinhumannonsmallcelllungcancercelllines
AT forrestalistairrr mir107andmir185caninducecellcyclearrestinhumannonsmallcelllungcancercelllines
AT maenoemi mir107andmir185caninducecellcyclearrestinhumannonsmallcelllungcancercelllines
AT hashimototakehiro mir107andmir185caninducecellcyclearrestinhumannonsmallcelllungcancercelllines
AT daubcarsteno mir107andmir185caninducecellcyclearrestinhumannonsmallcelllungcancercelllines
AT yasudajun mir107andmir185caninducecellcyclearrestinhumannonsmallcelllungcancercelllines