Detecting SNPs and estimating allele frequencies in clonal bacterial populations by sequencing pooled DNA

Summary: Here, we present a method for estimating the frequencies of SNP alleles present within pooled samples of DNA using high-throughput short-read sequencing. The method was tested on real data from six strains of the highly monomorphic pathogen Salmonella Paratyphi A, sequenced individually and...

Descripción completa

Detalles Bibliográficos
Autores principales: Holt, Kathryn E., Teo, Yik Y., Li, Heng, Nair, Satheesh, Dougan, Gordon, Wain, John, Parkhill, Julian
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Applications Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2722999/
https://www.ncbi.nlm.nih.gov/pubmed/19497932
http://dx.doi.org/10.1093/bioinformatics/btp344

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2722999/
https://www.ncbi.nlm.nih.gov/pubmed/19497932
http://dx.doi.org/10.1093/bioinformatics/btp344

Ejemplares similares