An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum

An asd-deleted (Δasd) mutant of Salmonella enterica serovar Pullorum (SP) was constructed using an improved method of gene knockout by combining the π-suicide plasmid system with the Red Disruption system. The asd gene was efficiently knocked out by the recombinant suicide vector, which replaced the...

Descripción completa

Detalles Bibliográficos
Autores principales: Geng, Shi-Zhong, Jiao, Xin-An, Pan, Zhi-Ming, Chen, Xiao-Juan, Zhang, Xiao-Ming, Chen, Xiang
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2009
Materias:
Methodology Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2723734/
https://www.ncbi.nlm.nih.gov/pubmed/19672470
http://dx.doi.org/10.1155/2009/646380
Descripción
Sumario:An asd-deleted (Δasd) mutant of Salmonella enterica serovar Pullorum (SP) was constructed using an improved method of gene knockout by combining the π-suicide plasmid system with the Red Disruption system. The asd gene was efficiently knocked out by the recombinant suicide vector, which replaced the asd gene with the Cm(R) gene. Based on the balanced lethal host-vector system, the phenotype of the Δasd mutant was further defined. The improved method was simpler and more effective than previously reported conventional methods.

Ejemplares similares