An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum

An asd-deleted (Δasd) mutant of Salmonella enterica serovar Pullorum (SP) was constructed using an improved method of gene knockout by combining the π-suicide plasmid system with the Red Disruption system. The asd gene was efficiently knocked out by the recombinant suicide vector, which replaced the...

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Autores principales: Geng, Shi-Zhong, Jiao, Xin-An, Pan, Zhi-Ming, Chen, Xiao-Juan, Zhang, Xiao-Ming, Chen, Xiang
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2009
Materias:
Methodology Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2723734/
https://www.ncbi.nlm.nih.gov/pubmed/19672470
http://dx.doi.org/10.1155/2009/646380
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author Geng, Shi-Zhong
Jiao, Xin-An
Pan, Zhi-Ming
Chen, Xiao-Juan
Zhang, Xiao-Ming
Chen, Xiang
author_facet Geng, Shi-Zhong
Jiao, Xin-An
Pan, Zhi-Ming
Chen, Xiao-Juan
Zhang, Xiao-Ming
Chen, Xiang
author_sort Geng, Shi-Zhong
collection PubMed
description An asd-deleted (Δasd) mutant of Salmonella enterica serovar Pullorum (SP) was constructed using an improved method of gene knockout by combining the π-suicide plasmid system with the Red Disruption system. The asd gene was efficiently knocked out by the recombinant suicide vector, which replaced the asd gene with the Cm(R) gene. Based on the balanced lethal host-vector system, the phenotype of the Δasd mutant was further defined. The improved method was simpler and more effective than previously reported conventional methods.
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spelling pubmed-27237342009-08-11 An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum Geng, Shi-Zhong Jiao, Xin-An Pan, Zhi-Ming Chen, Xiao-Juan Zhang, Xiao-Ming Chen, Xiang J Biomed Biotechnol Methodology Report An asd-deleted (Δasd) mutant of Salmonella enterica serovar Pullorum (SP) was constructed using an improved method of gene knockout by combining the π-suicide plasmid system with the Red Disruption system. The asd gene was efficiently knocked out by the recombinant suicide vector, which replaced the asd gene with the Cm(R) gene. Based on the balanced lethal host-vector system, the phenotype of the Δasd mutant was further defined. The improved method was simpler and more effective than previously reported conventional methods. Hindawi Publishing Corporation 2009 2009-08-09 /pmc/articles/PMC2723734/ /pubmed/19672470 http://dx.doi.org/10.1155/2009/646380 Text en Copyright © 2009 Shi-Zhong Geng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology Report
Geng, Shi-Zhong
Jiao, Xin-An
Pan, Zhi-Ming
Chen, Xiao-Juan
Zhang, Xiao-Ming
Chen, Xiang
An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum
title An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum
title_full An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum
title_fullStr An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum
title_full_unstemmed An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum
title_short An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum
title_sort improved method to knock out the asd gene of salmonella enterica serovar pullorum
topic Methodology Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2723734/
https://www.ncbi.nlm.nih.gov/pubmed/19672470
http://dx.doi.org/10.1155/2009/646380
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