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An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum

An asd-deleted (Δasd) mutant of Salmonella enterica serovar Pullorum (SP) was constructed using an improved method of gene knockout by combining the π-suicide plasmid system with the Red Disruption system. The asd gene was efficiently knocked out by the recombinant suicide vector, which replaced the...

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Detalles Bibliográficos
Autores principales:	Geng, Shi-Zhong, Jiao, Xin-An, Pan, Zhi-Ming, Chen, Xiao-Juan, Zhang, Xiao-Ming, Chen, Xiang
Formato:	Texto
Lenguaje:	English
Publicado:	Hindawi Publishing Corporation 2009
Materias:	Methodology Report
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2723734/ https://www.ncbi.nlm.nih.gov/pubmed/19672470 http://dx.doi.org/10.1155/2009/646380

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