Protein-Protein Interactions of Tandem Affinity Purified Protein Kinases from Rice

Eighty-eight rice (Oryza sativa) cDNAs encoding rice leaf expressed protein kinases (PKs) were fused to a Tandem Affinity Purification tag (TAP-tag) and expressed in transgenic rice plants. The TAP-tagged PKs and interacting proteins were purified from the T1 progeny of the transgenic rice plants an...

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Detalles Bibliográficos
Autores principales: Rohila, Jai S., Chen, Mei, Chen, Shuo, Chen, Johann, Cerny, Ronald L., Dardick, Christopher, Canlas, Patrick, Fujii, Hiroaki, Gribskov, Michael, Kanrar, Siddhartha, Knoflicek, Lucas, Stevenson, Becky, Xie, Mingtang, Xu, Xia, Zheng, Xianwu, Zhu, Jian-Kang, Ronald, Pamela, Fromm, Michael E.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2723914/
https://www.ncbi.nlm.nih.gov/pubmed/19690613
http://dx.doi.org/10.1371/journal.pone.0006685
Descripción
Sumario:Eighty-eight rice (Oryza sativa) cDNAs encoding rice leaf expressed protein kinases (PKs) were fused to a Tandem Affinity Purification tag (TAP-tag) and expressed in transgenic rice plants. The TAP-tagged PKs and interacting proteins were purified from the T1 progeny of the transgenic rice plants and identified by tandem mass spectrometry. Forty-five TAP-tagged PKs were recovered in this study and thirteen of these were found to interact with other rice proteins with a high probability score. In vivo phosphorylated sites were found for three of the PKs. A comparison of the TAP-tagged data from a combined analysis of 129 TAP-tagged rice protein kinases with a concurrent screen using yeast two hybrid methods identified an evolutionarily new rice protein that interacts with the well conserved cell division cycle 2 (CDC2) protein complex.

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