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Kinetic and thermodynamic characterization of single-mismatch discrimination using single-molecule imaging

A single-molecule detection setup based on total internal reflection fluorescence (TIRF) microscopy has been used to investigate association and dissociation kinetics of unlabeled 30mer DNA strands. Single-molecule sensitivity was accomplished by letting unlabeled DNA target strands mediate the bind...

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Detalles Bibliográficos
Autores principales:	Gunnarsson, Anders, Jönsson, Peter, Zhdanov, Vladimir P., Höök, Fredrik
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2009
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2724293/ https://www.ncbi.nlm.nih.gov/pubmed/19509313 http://dx.doi.org/10.1093/nar/gkp487

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