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Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureus

In this article, a panel of ssDNA aptamers specific to Staphylococcus aureus was obtained by a whole bacterium-based SELEX procedure and applied to probing S. aureus. After several rounds of selection with S. aureus as the target and Streptococcus and S. epidermidis as counter targets, the highly en...

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Autores principales: Cao, Xiaoxiao, Li, Shaohua, Chen, Liucun, Ding, Hongmei, Xu, Hua, Huang, Yanping, Li, Jie, Liu, Nongle, Cao, Weihong, Zhu, Yanjun, Shen, Beifen, Shao, Ningsheng
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2724295/
https://www.ncbi.nlm.nih.gov/pubmed/19498077
http://dx.doi.org/10.1093/nar/gkp489
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author Cao, Xiaoxiao
Li, Shaohua
Chen, Liucun
Ding, Hongmei
Xu, Hua
Huang, Yanping
Li, Jie
Liu, Nongle
Cao, Weihong
Zhu, Yanjun
Shen, Beifen
Shao, Ningsheng
author_facet Cao, Xiaoxiao
Li, Shaohua
Chen, Liucun
Ding, Hongmei
Xu, Hua
Huang, Yanping
Li, Jie
Liu, Nongle
Cao, Weihong
Zhu, Yanjun
Shen, Beifen
Shao, Ningsheng
author_sort Cao, Xiaoxiao
collection PubMed
description In this article, a panel of ssDNA aptamers specific to Staphylococcus aureus was obtained by a whole bacterium-based SELEX procedure and applied to probing S. aureus. After several rounds of selection with S. aureus as the target and Streptococcus and S. epidermidis as counter targets, the highly enriched oligonucleic acid pool was sequenced and then grouped under different families on the basis of the homology of the primary sequence and the similarity of the secondary structure. Eleven sequences from different families were selected for further characterization by confocal imaging and flow cytometry analysis. Results showed that five aptamers demonstrated high specificity and affinity to S. aureus individually. The five aptamers recognize different molecular targets by competitive experiment. Combining these five aptamers had a much better effect than the individual aptamer in the recognition of different S. aureus strains. In addition, the combined aptamers can probe single S. aureus in pyogenic fluids. Our work demonstrates that a set of aptamers specific to one bacterium can be used in combination for the identification of the bacterium instead of a single aptamer.
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spelling pubmed-27242952009-08-18 Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureus Cao, Xiaoxiao Li, Shaohua Chen, Liucun Ding, Hongmei Xu, Hua Huang, Yanping Li, Jie Liu, Nongle Cao, Weihong Zhu, Yanjun Shen, Beifen Shao, Ningsheng Nucleic Acids Res Molecular Biology In this article, a panel of ssDNA aptamers specific to Staphylococcus aureus was obtained by a whole bacterium-based SELEX procedure and applied to probing S. aureus. After several rounds of selection with S. aureus as the target and Streptococcus and S. epidermidis as counter targets, the highly enriched oligonucleic acid pool was sequenced and then grouped under different families on the basis of the homology of the primary sequence and the similarity of the secondary structure. Eleven sequences from different families were selected for further characterization by confocal imaging and flow cytometry analysis. Results showed that five aptamers demonstrated high specificity and affinity to S. aureus individually. The five aptamers recognize different molecular targets by competitive experiment. Combining these five aptamers had a much better effect than the individual aptamer in the recognition of different S. aureus strains. In addition, the combined aptamers can probe single S. aureus in pyogenic fluids. Our work demonstrates that a set of aptamers specific to one bacterium can be used in combination for the identification of the bacterium instead of a single aptamer. Oxford University Press 2009-08 2009-06-04 /pmc/articles/PMC2724295/ /pubmed/19498077 http://dx.doi.org/10.1093/nar/gkp489 Text en © 2009 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Molecular Biology
Cao, Xiaoxiao
Li, Shaohua
Chen, Liucun
Ding, Hongmei
Xu, Hua
Huang, Yanping
Li, Jie
Liu, Nongle
Cao, Weihong
Zhu, Yanjun
Shen, Beifen
Shao, Ningsheng
Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureus
title Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureus
title_full Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureus
title_fullStr Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureus
title_full_unstemmed Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureus
title_short Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureus
title_sort combining use of a panel of ssdna aptamers in the detection of staphylococcus aureus
topic Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2724295/
https://www.ncbi.nlm.nih.gov/pubmed/19498077
http://dx.doi.org/10.1093/nar/gkp489
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