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Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureus
In this article, a panel of ssDNA aptamers specific to Staphylococcus aureus was obtained by a whole bacterium-based SELEX procedure and applied to probing S. aureus. After several rounds of selection with S. aureus as the target and Streptococcus and S. epidermidis as counter targets, the highly en...
Autores principales: | , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2724295/ https://www.ncbi.nlm.nih.gov/pubmed/19498077 http://dx.doi.org/10.1093/nar/gkp489 |
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author | Cao, Xiaoxiao Li, Shaohua Chen, Liucun Ding, Hongmei Xu, Hua Huang, Yanping Li, Jie Liu, Nongle Cao, Weihong Zhu, Yanjun Shen, Beifen Shao, Ningsheng |
author_facet | Cao, Xiaoxiao Li, Shaohua Chen, Liucun Ding, Hongmei Xu, Hua Huang, Yanping Li, Jie Liu, Nongle Cao, Weihong Zhu, Yanjun Shen, Beifen Shao, Ningsheng |
author_sort | Cao, Xiaoxiao |
collection | PubMed |
description | In this article, a panel of ssDNA aptamers specific to Staphylococcus aureus was obtained by a whole bacterium-based SELEX procedure and applied to probing S. aureus. After several rounds of selection with S. aureus as the target and Streptococcus and S. epidermidis as counter targets, the highly enriched oligonucleic acid pool was sequenced and then grouped under different families on the basis of the homology of the primary sequence and the similarity of the secondary structure. Eleven sequences from different families were selected for further characterization by confocal imaging and flow cytometry analysis. Results showed that five aptamers demonstrated high specificity and affinity to S. aureus individually. The five aptamers recognize different molecular targets by competitive experiment. Combining these five aptamers had a much better effect than the individual aptamer in the recognition of different S. aureus strains. In addition, the combined aptamers can probe single S. aureus in pyogenic fluids. Our work demonstrates that a set of aptamers specific to one bacterium can be used in combination for the identification of the bacterium instead of a single aptamer. |
format | Text |
id | pubmed-2724295 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-27242952009-08-18 Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureus Cao, Xiaoxiao Li, Shaohua Chen, Liucun Ding, Hongmei Xu, Hua Huang, Yanping Li, Jie Liu, Nongle Cao, Weihong Zhu, Yanjun Shen, Beifen Shao, Ningsheng Nucleic Acids Res Molecular Biology In this article, a panel of ssDNA aptamers specific to Staphylococcus aureus was obtained by a whole bacterium-based SELEX procedure and applied to probing S. aureus. After several rounds of selection with S. aureus as the target and Streptococcus and S. epidermidis as counter targets, the highly enriched oligonucleic acid pool was sequenced and then grouped under different families on the basis of the homology of the primary sequence and the similarity of the secondary structure. Eleven sequences from different families were selected for further characterization by confocal imaging and flow cytometry analysis. Results showed that five aptamers demonstrated high specificity and affinity to S. aureus individually. The five aptamers recognize different molecular targets by competitive experiment. Combining these five aptamers had a much better effect than the individual aptamer in the recognition of different S. aureus strains. In addition, the combined aptamers can probe single S. aureus in pyogenic fluids. Our work demonstrates that a set of aptamers specific to one bacterium can be used in combination for the identification of the bacterium instead of a single aptamer. Oxford University Press 2009-08 2009-06-04 /pmc/articles/PMC2724295/ /pubmed/19498077 http://dx.doi.org/10.1093/nar/gkp489 Text en © 2009 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Molecular Biology Cao, Xiaoxiao Li, Shaohua Chen, Liucun Ding, Hongmei Xu, Hua Huang, Yanping Li, Jie Liu, Nongle Cao, Weihong Zhu, Yanjun Shen, Beifen Shao, Ningsheng Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureus |
title | Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureus |
title_full | Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureus |
title_fullStr | Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureus |
title_full_unstemmed | Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureus |
title_short | Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureus |
title_sort | combining use of a panel of ssdna aptamers in the detection of staphylococcus aureus |
topic | Molecular Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2724295/ https://www.ncbi.nlm.nih.gov/pubmed/19498077 http://dx.doi.org/10.1093/nar/gkp489 |
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