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Biosensor immunoassay for traces of hazelnut protein in olive oil

The fraudulent addition of hazelnut oil to more expensive olive oil not only causes economical loss but may also result in problems for allergic individuals as they may inadvertently be exposed to potentially allergenic hazelnut proteins. To improve consumer safety, a rapid and sensitive direct bios...

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Detalles Bibliográficos
Autores principales: Bremer, Maria G. E. G., Smits, Nathalie G. E., Haasnoot, Willem
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2724632/
https://www.ncbi.nlm.nih.gov/pubmed/19263041
http://dx.doi.org/10.1007/s00216-009-2720-1
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author Bremer, Maria G. E. G.
Smits, Nathalie G. E.
Haasnoot, Willem
author_facet Bremer, Maria G. E. G.
Smits, Nathalie G. E.
Haasnoot, Willem
author_sort Bremer, Maria G. E. G.
collection PubMed
description The fraudulent addition of hazelnut oil to more expensive olive oil not only causes economical loss but may also result in problems for allergic individuals as they may inadvertently be exposed to potentially allergenic hazelnut proteins. To improve consumer safety, a rapid and sensitive direct biosensor immunoassay, based on a highly specific monoclonal antibody, was developed to detect the presence of hazelnut proteins in olive oils. The sample preparation was easy (extraction with buffer); the assay time was fast (4.5 min only) and the limit of detection was low (0.08 μg/g of hazelnut proteins in olive oil). Recoveries obtained with an olive oil mixed with different amounts of a hazelnut protein containing hazelnut oil varied between 93% and 109%. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00216-009-2720-1) contains supplementary material, which is available to authorized users.
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spelling pubmed-27246322009-08-13 Biosensor immunoassay for traces of hazelnut protein in olive oil Bremer, Maria G. E. G. Smits, Nathalie G. E. Haasnoot, Willem Anal Bioanal Chem Original Paper The fraudulent addition of hazelnut oil to more expensive olive oil not only causes economical loss but may also result in problems for allergic individuals as they may inadvertently be exposed to potentially allergenic hazelnut proteins. To improve consumer safety, a rapid and sensitive direct biosensor immunoassay, based on a highly specific monoclonal antibody, was developed to detect the presence of hazelnut proteins in olive oils. The sample preparation was easy (extraction with buffer); the assay time was fast (4.5 min only) and the limit of detection was low (0.08 μg/g of hazelnut proteins in olive oil). Recoveries obtained with an olive oil mixed with different amounts of a hazelnut protein containing hazelnut oil varied between 93% and 109%. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00216-009-2720-1) contains supplementary material, which is available to authorized users. Springer-Verlag 2009-03-05 2009 /pmc/articles/PMC2724632/ /pubmed/19263041 http://dx.doi.org/10.1007/s00216-009-2720-1 Text en © The Author(s) 2009 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Original Paper
Bremer, Maria G. E. G.
Smits, Nathalie G. E.
Haasnoot, Willem
Biosensor immunoassay for traces of hazelnut protein in olive oil
title Biosensor immunoassay for traces of hazelnut protein in olive oil
title_full Biosensor immunoassay for traces of hazelnut protein in olive oil
title_fullStr Biosensor immunoassay for traces of hazelnut protein in olive oil
title_full_unstemmed Biosensor immunoassay for traces of hazelnut protein in olive oil
title_short Biosensor immunoassay for traces of hazelnut protein in olive oil
title_sort biosensor immunoassay for traces of hazelnut protein in olive oil
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2724632/
https://www.ncbi.nlm.nih.gov/pubmed/19263041
http://dx.doi.org/10.1007/s00216-009-2720-1
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