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Biosensor immunoassay for traces of hazelnut protein in olive oil
The fraudulent addition of hazelnut oil to more expensive olive oil not only causes economical loss but may also result in problems for allergic individuals as they may inadvertently be exposed to potentially allergenic hazelnut proteins. To improve consumer safety, a rapid and sensitive direct bios...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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Springer-Verlag
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2724632/ https://www.ncbi.nlm.nih.gov/pubmed/19263041 http://dx.doi.org/10.1007/s00216-009-2720-1 |
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author | Bremer, Maria G. E. G. Smits, Nathalie G. E. Haasnoot, Willem |
author_facet | Bremer, Maria G. E. G. Smits, Nathalie G. E. Haasnoot, Willem |
author_sort | Bremer, Maria G. E. G. |
collection | PubMed |
description | The fraudulent addition of hazelnut oil to more expensive olive oil not only causes economical loss but may also result in problems for allergic individuals as they may inadvertently be exposed to potentially allergenic hazelnut proteins. To improve consumer safety, a rapid and sensitive direct biosensor immunoassay, based on a highly specific monoclonal antibody, was developed to detect the presence of hazelnut proteins in olive oils. The sample preparation was easy (extraction with buffer); the assay time was fast (4.5 min only) and the limit of detection was low (0.08 μg/g of hazelnut proteins in olive oil). Recoveries obtained with an olive oil mixed with different amounts of a hazelnut protein containing hazelnut oil varied between 93% and 109%. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00216-009-2720-1) contains supplementary material, which is available to authorized users. |
format | Text |
id | pubmed-2724632 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Springer-Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-27246322009-08-13 Biosensor immunoassay for traces of hazelnut protein in olive oil Bremer, Maria G. E. G. Smits, Nathalie G. E. Haasnoot, Willem Anal Bioanal Chem Original Paper The fraudulent addition of hazelnut oil to more expensive olive oil not only causes economical loss but may also result in problems for allergic individuals as they may inadvertently be exposed to potentially allergenic hazelnut proteins. To improve consumer safety, a rapid and sensitive direct biosensor immunoassay, based on a highly specific monoclonal antibody, was developed to detect the presence of hazelnut proteins in olive oils. The sample preparation was easy (extraction with buffer); the assay time was fast (4.5 min only) and the limit of detection was low (0.08 μg/g of hazelnut proteins in olive oil). Recoveries obtained with an olive oil mixed with different amounts of a hazelnut protein containing hazelnut oil varied between 93% and 109%. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00216-009-2720-1) contains supplementary material, which is available to authorized users. Springer-Verlag 2009-03-05 2009 /pmc/articles/PMC2724632/ /pubmed/19263041 http://dx.doi.org/10.1007/s00216-009-2720-1 Text en © The Author(s) 2009 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Original Paper Bremer, Maria G. E. G. Smits, Nathalie G. E. Haasnoot, Willem Biosensor immunoassay for traces of hazelnut protein in olive oil |
title | Biosensor immunoassay for traces of hazelnut protein in olive oil |
title_full | Biosensor immunoassay for traces of hazelnut protein in olive oil |
title_fullStr | Biosensor immunoassay for traces of hazelnut protein in olive oil |
title_full_unstemmed | Biosensor immunoassay for traces of hazelnut protein in olive oil |
title_short | Biosensor immunoassay for traces of hazelnut protein in olive oil |
title_sort | biosensor immunoassay for traces of hazelnut protein in olive oil |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2724632/ https://www.ncbi.nlm.nih.gov/pubmed/19263041 http://dx.doi.org/10.1007/s00216-009-2720-1 |
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