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Salt stress-induced alterations in the root proteome of barley genotypes with contrasting response towards salinity

In addition to drought and extreme temperatures, soil salinity represents a growing threat to crop productivity. Among the cereal crops, barley is considered as notably salt tolerant, and cultivars show considerable variation for tolerance towards salinity stress. In order to unravel the molecular m...

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Autores principales: Witzel, Katja, Weidner, Annette, Surabhi, Giridara-Kumar, Börner, Andreas, Mock, Hans-Peter
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2724703/
https://www.ncbi.nlm.nih.gov/pubmed/19671579
http://dx.doi.org/10.1093/jxb/erp198
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author Witzel, Katja
Weidner, Annette
Surabhi, Giridara-Kumar
Börner, Andreas
Mock, Hans-Peter
author_facet Witzel, Katja
Weidner, Annette
Surabhi, Giridara-Kumar
Börner, Andreas
Mock, Hans-Peter
author_sort Witzel, Katja
collection PubMed
description In addition to drought and extreme temperatures, soil salinity represents a growing threat to crop productivity. Among the cereal crops, barley is considered as notably salt tolerant, and cultivars show considerable variation for tolerance towards salinity stress. In order to unravel the molecular mechanisms underlying salt stress tolerance and to utilize the natural genetic variation of barley accessions, a series of hydroponics-based salinity stress experiments was conducted using two genetic mapping parents, cvs Steptoe and Morex, which display contrasting levels of salinity tolerance. The proteome of roots from both genotypes was investigated as displayed by two-dimensional gel electrophoresis, and comparisons were made between plants grown under non-saline and saline conditions. Multivariate analysis of the resulting protein patterns revealed cultivar-specific and salt stress-responsive protein expression. Mass spectrometry-based identification was successful for 26 out of 39 selected protein spots. Hierarchical clustering was applied to detect similar protein expression patterns. Among those, two proteins involved in the glutathione-based detoxification of reactive oxygen species (ROS) were more abundant in the tolerant genotype, while proteins involved in iron uptake were expressed at a higher level in the sensitive one. This study emphasizes the role of proteins involved in ROS detoxification during salinity stress, and identified potential candidates for increasing salt tolerance in barley.
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spelling pubmed-27247032009-08-20 Salt stress-induced alterations in the root proteome of barley genotypes with contrasting response towards salinity Witzel, Katja Weidner, Annette Surabhi, Giridara-Kumar Börner, Andreas Mock, Hans-Peter J Exp Bot Research Papers In addition to drought and extreme temperatures, soil salinity represents a growing threat to crop productivity. Among the cereal crops, barley is considered as notably salt tolerant, and cultivars show considerable variation for tolerance towards salinity stress. In order to unravel the molecular mechanisms underlying salt stress tolerance and to utilize the natural genetic variation of barley accessions, a series of hydroponics-based salinity stress experiments was conducted using two genetic mapping parents, cvs Steptoe and Morex, which display contrasting levels of salinity tolerance. The proteome of roots from both genotypes was investigated as displayed by two-dimensional gel electrophoresis, and comparisons were made between plants grown under non-saline and saline conditions. Multivariate analysis of the resulting protein patterns revealed cultivar-specific and salt stress-responsive protein expression. Mass spectrometry-based identification was successful for 26 out of 39 selected protein spots. Hierarchical clustering was applied to detect similar protein expression patterns. Among those, two proteins involved in the glutathione-based detoxification of reactive oxygen species (ROS) were more abundant in the tolerant genotype, while proteins involved in iron uptake were expressed at a higher level in the sensitive one. This study emphasizes the role of proteins involved in ROS detoxification during salinity stress, and identified potential candidates for increasing salt tolerance in barley. Oxford University Press 2009-08 /pmc/articles/PMC2724703/ /pubmed/19671579 http://dx.doi.org/10.1093/jxb/erp198 Text en © 2009 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)
spellingShingle Research Papers
Witzel, Katja
Weidner, Annette
Surabhi, Giridara-Kumar
Börner, Andreas
Mock, Hans-Peter
Salt stress-induced alterations in the root proteome of barley genotypes with contrasting response towards salinity
title Salt stress-induced alterations in the root proteome of barley genotypes with contrasting response towards salinity
title_full Salt stress-induced alterations in the root proteome of barley genotypes with contrasting response towards salinity
title_fullStr Salt stress-induced alterations in the root proteome of barley genotypes with contrasting response towards salinity
title_full_unstemmed Salt stress-induced alterations in the root proteome of barley genotypes with contrasting response towards salinity
title_short Salt stress-induced alterations in the root proteome of barley genotypes with contrasting response towards salinity
title_sort salt stress-induced alterations in the root proteome of barley genotypes with contrasting response towards salinity
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2724703/
https://www.ncbi.nlm.nih.gov/pubmed/19671579
http://dx.doi.org/10.1093/jxb/erp198
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