Similar Biochemical Signatures and Prion Protein Genotypes in Atypical Scrapie and Nor98 Cases, France and Norway

Isolates of atypical scrapie recently identified in sheep and goats in France were compared with Nor98 isolates reported in Norway. Western blot methods for characterization of the protease-resistant prion protein showed that all these isolates shared a unique biochemical signature: 5 groups of band...

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Autores principales: Arsac, Jean-Noël, Andreoletti, Olivier, Bilheude, Jean-Marc, Lacroux, Caroline, Benestad, Sylvie L., Baron, Thierry
Formato: Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2007
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2725815/
https://www.ncbi.nlm.nih.gov/pubmed/17370516
http://dx.doi.org/10.3201/eid1301.060393
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author Arsac, Jean-Noël
Andreoletti, Olivier
Bilheude, Jean-Marc
Lacroux, Caroline
Benestad, Sylvie L.
Baron, Thierry
author_facet Arsac, Jean-Noël
Andreoletti, Olivier
Bilheude, Jean-Marc
Lacroux, Caroline
Benestad, Sylvie L.
Baron, Thierry
author_sort Arsac, Jean-Noël
collection PubMed
description Isolates of atypical scrapie recently identified in sheep and goats in France were compared with Nor98 isolates reported in Norway. Western blot methods for characterization of the protease-resistant prion protein showed that all these isolates shared a unique biochemical signature: 5 groups of bands, including a characteristic band of apparent low molecular weight (11 kDa). This pattern could originate from the presence of 3 different protease cleavage products, including the 11 kDa most likely cleaved at both N- and C-sides of the protein. Genetic data, which strongly suggested the higher susceptibility of AHQ and AF(141)RQ animals in French cases, resembled earlier data from Nor98 scrapie.
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spelling pubmed-27258152009-09-10 Similar Biochemical Signatures and Prion Protein Genotypes in Atypical Scrapie and Nor98 Cases, France and Norway Arsac, Jean-Noël Andreoletti, Olivier Bilheude, Jean-Marc Lacroux, Caroline Benestad, Sylvie L. Baron, Thierry Emerg Infect Dis Dispatch Isolates of atypical scrapie recently identified in sheep and goats in France were compared with Nor98 isolates reported in Norway. Western blot methods for characterization of the protease-resistant prion protein showed that all these isolates shared a unique biochemical signature: 5 groups of bands, including a characteristic band of apparent low molecular weight (11 kDa). This pattern could originate from the presence of 3 different protease cleavage products, including the 11 kDa most likely cleaved at both N- and C-sides of the protein. Genetic data, which strongly suggested the higher susceptibility of AHQ and AF(141)RQ animals in French cases, resembled earlier data from Nor98 scrapie. Centers for Disease Control and Prevention 2007-01 /pmc/articles/PMC2725815/ /pubmed/17370516 http://dx.doi.org/10.3201/eid1301.060393 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
Arsac, Jean-Noël
Andreoletti, Olivier
Bilheude, Jean-Marc
Lacroux, Caroline
Benestad, Sylvie L.
Baron, Thierry
Similar Biochemical Signatures and Prion Protein Genotypes in Atypical Scrapie and Nor98 Cases, France and Norway
title Similar Biochemical Signatures and Prion Protein Genotypes in Atypical Scrapie and Nor98 Cases, France and Norway
title_full Similar Biochemical Signatures and Prion Protein Genotypes in Atypical Scrapie and Nor98 Cases, France and Norway
title_fullStr Similar Biochemical Signatures and Prion Protein Genotypes in Atypical Scrapie and Nor98 Cases, France and Norway
title_full_unstemmed Similar Biochemical Signatures and Prion Protein Genotypes in Atypical Scrapie and Nor98 Cases, France and Norway
title_short Similar Biochemical Signatures and Prion Protein Genotypes in Atypical Scrapie and Nor98 Cases, France and Norway
title_sort similar biochemical signatures and prion protein genotypes in atypical scrapie and nor98 cases, france and norway
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2725815/
https://www.ncbi.nlm.nih.gov/pubmed/17370516
http://dx.doi.org/10.3201/eid1301.060393
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