Rapid Genome Sequencing of RNA Viruses

We developed a system for rapid determination of viral RNA sequences whereby genomic sequence is obtained from cultured virus isolates without subcloning into plasmid vectors. This method affords new opportunities to address the challenges of unknown or untypeable emerging viruses.

Detalles Bibliográficos
Autores principales: Mizutani, Tetsuya, Endoh, Daiji, Okamoto, Michiko, Shirato, Kazuya, Shimizu, Hiroyuki, Arita, Minetaro, Fukushi, Shuetsu, Saijo, Masayuki, Lim, Chang Kweng, Ito, Mikako, Nerome, Reiko, Takasaki, Tomohiko, Ishii, Koji, Suzuki, Tetsuro, Kurane, Ichiro, Morikawa, Shigeru, Nishimura, Hidekazu
Formato: Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2007
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2725858/
https://www.ncbi.nlm.nih.gov/pubmed/17479903
http://dx.doi.org/10.3201/eid1302.061032
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author Mizutani, Tetsuya
Endoh, Daiji
Okamoto, Michiko
Shirato, Kazuya
Shimizu, Hiroyuki
Arita, Minetaro
Fukushi, Shuetsu
Saijo, Masayuki
Lim, Chang Kweng
Ito, Mikako
Nerome, Reiko
Takasaki, Tomohiko
Ishii, Koji
Suzuki, Tetsuro
Kurane, Ichiro
Morikawa, Shigeru
Nishimura, Hidekazu
author_facet Mizutani, Tetsuya
Endoh, Daiji
Okamoto, Michiko
Shirato, Kazuya
Shimizu, Hiroyuki
Arita, Minetaro
Fukushi, Shuetsu
Saijo, Masayuki
Lim, Chang Kweng
Ito, Mikako
Nerome, Reiko
Takasaki, Tomohiko
Ishii, Koji
Suzuki, Tetsuro
Kurane, Ichiro
Morikawa, Shigeru
Nishimura, Hidekazu
author_sort Mizutani, Tetsuya
collection PubMed
description We developed a system for rapid determination of viral RNA sequences whereby genomic sequence is obtained from cultured virus isolates without subcloning into plasmid vectors. This method affords new opportunities to address the challenges of unknown or untypeable emerging viruses.
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spelling pubmed-27258582009-09-10 Rapid Genome Sequencing of RNA Viruses Mizutani, Tetsuya Endoh, Daiji Okamoto, Michiko Shirato, Kazuya Shimizu, Hiroyuki Arita, Minetaro Fukushi, Shuetsu Saijo, Masayuki Lim, Chang Kweng Ito, Mikako Nerome, Reiko Takasaki, Tomohiko Ishii, Koji Suzuki, Tetsuro Kurane, Ichiro Morikawa, Shigeru Nishimura, Hidekazu Emerg Infect Dis Dispatch We developed a system for rapid determination of viral RNA sequences whereby genomic sequence is obtained from cultured virus isolates without subcloning into plasmid vectors. This method affords new opportunities to address the challenges of unknown or untypeable emerging viruses. Centers for Disease Control and Prevention 2007-02 /pmc/articles/PMC2725858/ /pubmed/17479903 http://dx.doi.org/10.3201/eid1302.061032 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
Mizutani, Tetsuya
Endoh, Daiji
Okamoto, Michiko
Shirato, Kazuya
Shimizu, Hiroyuki
Arita, Minetaro
Fukushi, Shuetsu
Saijo, Masayuki
Lim, Chang Kweng
Ito, Mikako
Nerome, Reiko
Takasaki, Tomohiko
Ishii, Koji
Suzuki, Tetsuro
Kurane, Ichiro
Morikawa, Shigeru
Nishimura, Hidekazu
Rapid Genome Sequencing of RNA Viruses
title Rapid Genome Sequencing of RNA Viruses
title_full Rapid Genome Sequencing of RNA Viruses
title_fullStr Rapid Genome Sequencing of RNA Viruses
title_full_unstemmed Rapid Genome Sequencing of RNA Viruses
title_short Rapid Genome Sequencing of RNA Viruses
title_sort rapid genome sequencing of rna viruses
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2725858/
https://www.ncbi.nlm.nih.gov/pubmed/17479903
http://dx.doi.org/10.3201/eid1302.061032
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