DySCo: Quantitating Associations of Membrane Proteins Using Two-Color Single-Molecule Tracking

We present a general method called dynamic single-molecule colocalization for quantitating the associations of single cell surface molecules labeled with distinct autofluorescent proteins. The chief advantages of the new quantitative approach are that, in addition to stable interactions, it is capab...

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Detalles Bibliográficos
Autores principales: Dunne, Paul D., Fernandes, Ricardo A., McColl, James, Yoon, Ji Won, James, John R., Davis, Simon J., Klenerman, David
Formato: Texto
Lenguaje:English
Publicado: The Biophysical Society 2009
Materias:
Biophysical Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2726305/
https://www.ncbi.nlm.nih.gov/pubmed/19686638
http://dx.doi.org/10.1016/j.bpj.2009.05.046
Descripción
Sumario:We present a general method called dynamic single-molecule colocalization for quantitating the associations of single cell surface molecules labeled with distinct autofluorescent proteins. The chief advantages of the new quantitative approach are that, in addition to stable interactions, it is capable of measuring nonconstitutive associations, such as those induced by the cytoskeleton, and it is applicable to situations where the number of molecules is small.

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