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Differential Bacterial Surface Display of Peptides by the Transmembrane Domain of OmpA

Peptide libraries or antigenic determinants can be displayed on the surface of bacteria through insertion in a suitable outer membrane scaffold protein. Here, we inserted the well-known antibody epitopes 3xFLAG and 2xmyc in exterior loops of the transmembrane (TM) domain of OmpA. Although these high...

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Detalles Bibliográficos
Autores principales: Verhoeven, Gertjan S., Alexeeva, Svetlana, Dogterom, Marileen, den Blaauwen, Tanneke
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2726941/
https://www.ncbi.nlm.nih.gov/pubmed/19707582
http://dx.doi.org/10.1371/journal.pone.0006739