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Stable generation of serum- and feeder-free embryonic stem cell-derived mice with full germline-competency by using a GSK3 specific inhibitor
C57BL/6 (B6)-derived embryonic stem (ES) cells are not widely used to generate knockout mice despite the advantage of a well-defined genetic background because of poor developmental potential. We newly established serum- and feeder-free B6 ES cells with full developmental potential by using leukemia...
Autores principales: | Sato, Hiromu, Amagai, Keiko, Shimizukawa, Rie, Tamai, Yoshitaka |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Wiley Subscription Services, Inc., A Wiley Company
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2726955/ https://www.ncbi.nlm.nih.gov/pubmed/19391115 http://dx.doi.org/10.1002/dvg.20514 |
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