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In vitro, but not in vivo, reversibility of peritoneal macrophages activation during experimental acute pancreatitis

BACKGROUND: Systemic inflammatory response syndrome is one of the major pathobiologic processes underlying severe acute pancreatitis and the degree of macrophage activation could be one of the factors that finally determine the severity of the disease. We evaluated the activation phenotype in perito...

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Autores principales: Gea-Sorlí, Sabrina, Closa, Daniel
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2727495/
https://www.ncbi.nlm.nih.gov/pubmed/19646232
http://dx.doi.org/10.1186/1471-2172-10-42
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author Gea-Sorlí, Sabrina
Closa, Daniel
author_facet Gea-Sorlí, Sabrina
Closa, Daniel
author_sort Gea-Sorlí, Sabrina
collection PubMed
description BACKGROUND: Systemic inflammatory response syndrome is one of the major pathobiologic processes underlying severe acute pancreatitis and the degree of macrophage activation could be one of the factors that finally determine the severity of the disease. We evaluated the activation phenotype in peritoneal macrophages during the progression of an experimental model of acute pancreatitis induced in rats by intraductal administration of 5% sodium taurocholate and the effect of IL-4 and IL-13 to modulate this activation. Samples of pancreas, lung and adipose tissue as well as plasma were also obtained. In some animals IL4 and IL13 were injected 1 h after induction in order to modulate macrophage activation. The expressions of TNFα and Mannose Receptor, as indicators of classical and alternative macrophage activation, were evaluated. Levels of myeloperoxidase and plasma lipase were determined to evaluate the severity of the inflammatory process. The stability of IL-4 in ascitic fluid and plasma was evaluated. RESULTS: Peritoneal macrophages showed a classical M1 activation clearly induced 3 h after pancreatitis induction and maintained until 18 h. Treatment with IL-4 and IL-13 reversed the activation of macrophages from a classical M1 to alternative M2 in vitro, but failed to modulate the response of peritoneal macrophages in vivo despite a reduction in inflammation was observed in lung and adipose tissue. Finally, IL-4 shows a short half-live in ascitic fluid when compared with plasma. CONCLUSION: Peritoneal macrophages adopt a pro-inflammatory activation early during acute pancreatitis. Treatment with M2 cytokines could revert in vitro the pancreatitis-induced activation of macrophages but fails to modulate its activation in vivo. This treatment has only a moderate effect in reducing the systemic inflammation associated to acute pancreatitis. Hydrolytic enzymes presents in ascitic fluid could be involved in the degradation of cytokines, strongly reducing its utility to modulate peritoneal macrophages in pancreatitis.
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spelling pubmed-27274952009-08-15 In vitro, but not in vivo, reversibility of peritoneal macrophages activation during experimental acute pancreatitis Gea-Sorlí, Sabrina Closa, Daniel BMC Immunol Research Article BACKGROUND: Systemic inflammatory response syndrome is one of the major pathobiologic processes underlying severe acute pancreatitis and the degree of macrophage activation could be one of the factors that finally determine the severity of the disease. We evaluated the activation phenotype in peritoneal macrophages during the progression of an experimental model of acute pancreatitis induced in rats by intraductal administration of 5% sodium taurocholate and the effect of IL-4 and IL-13 to modulate this activation. Samples of pancreas, lung and adipose tissue as well as plasma were also obtained. In some animals IL4 and IL13 were injected 1 h after induction in order to modulate macrophage activation. The expressions of TNFα and Mannose Receptor, as indicators of classical and alternative macrophage activation, were evaluated. Levels of myeloperoxidase and plasma lipase were determined to evaluate the severity of the inflammatory process. The stability of IL-4 in ascitic fluid and plasma was evaluated. RESULTS: Peritoneal macrophages showed a classical M1 activation clearly induced 3 h after pancreatitis induction and maintained until 18 h. Treatment with IL-4 and IL-13 reversed the activation of macrophages from a classical M1 to alternative M2 in vitro, but failed to modulate the response of peritoneal macrophages in vivo despite a reduction in inflammation was observed in lung and adipose tissue. Finally, IL-4 shows a short half-live in ascitic fluid when compared with plasma. CONCLUSION: Peritoneal macrophages adopt a pro-inflammatory activation early during acute pancreatitis. Treatment with M2 cytokines could revert in vitro the pancreatitis-induced activation of macrophages but fails to modulate its activation in vivo. This treatment has only a moderate effect in reducing the systemic inflammation associated to acute pancreatitis. Hydrolytic enzymes presents in ascitic fluid could be involved in the degradation of cytokines, strongly reducing its utility to modulate peritoneal macrophages in pancreatitis. BioMed Central 2009-07-31 /pmc/articles/PMC2727495/ /pubmed/19646232 http://dx.doi.org/10.1186/1471-2172-10-42 Text en Copyright © 2009 Gea-Sorlí and Closa; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gea-Sorlí, Sabrina
Closa, Daniel
In vitro, but not in vivo, reversibility of peritoneal macrophages activation during experimental acute pancreatitis
title In vitro, but not in vivo, reversibility of peritoneal macrophages activation during experimental acute pancreatitis
title_full In vitro, but not in vivo, reversibility of peritoneal macrophages activation during experimental acute pancreatitis
title_fullStr In vitro, but not in vivo, reversibility of peritoneal macrophages activation during experimental acute pancreatitis
title_full_unstemmed In vitro, but not in vivo, reversibility of peritoneal macrophages activation during experimental acute pancreatitis
title_short In vitro, but not in vivo, reversibility of peritoneal macrophages activation during experimental acute pancreatitis
title_sort in vitro, but not in vivo, reversibility of peritoneal macrophages activation during experimental acute pancreatitis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2727495/
https://www.ncbi.nlm.nih.gov/pubmed/19646232
http://dx.doi.org/10.1186/1471-2172-10-42
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