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Chlamydia trachomatis Alters Iron-Regulatory Protein-1 Binding Capacity and Modulates Cellular Iron Homeostasis in HeLa-229 Cells

Chlamydia trachomatis (CT) is the leading cause of diseases related to reproductive health and iron plays important role in chlamydial pathogenesis. Iron homeostasis in chlamydia-infected cells is not clear thus far. This study shows that expression of the transferrin receptor (TfR) is downregulated...

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Detalles Bibliográficos
Autores principales: Vardhan, Harsh, Bhengraj, Apurb R., Jha, Rajneesh, Singh Mittal, Aruna
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2727623/
https://www.ncbi.nlm.nih.gov/pubmed/19688112
http://dx.doi.org/10.1155/2009/342032
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author Vardhan, Harsh
Bhengraj, Apurb R.
Jha, Rajneesh
Singh Mittal, Aruna
author_facet Vardhan, Harsh
Bhengraj, Apurb R.
Jha, Rajneesh
Singh Mittal, Aruna
author_sort Vardhan, Harsh
collection PubMed
description Chlamydia trachomatis (CT) is the leading cause of diseases related to reproductive health and iron plays important role in chlamydial pathogenesis. Iron homeostasis in chlamydia-infected cells is not clear thus far. This study shows that expression of the transferrin receptor (TfR) is downregulated, whereas expression of the ferritin heavy chain is upregulated in CT-infected HeLa-229 cells. Expression of iron-regulatory protein (IRP)-1 predominates over IRP-2 in infected cells. In infected cells, attenuated binding activity of IRP-iron responsive elements (IREs) is observed using the electrophoretic mobility-shift assay. These results suggest that iron homeostasis is modulated in CT-infected HeLa cells at the interface of acquisition and commensal use of iron.
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spelling pubmed-27276232009-08-17 Chlamydia trachomatis Alters Iron-Regulatory Protein-1 Binding Capacity and Modulates Cellular Iron Homeostasis in HeLa-229 Cells Vardhan, Harsh Bhengraj, Apurb R. Jha, Rajneesh Singh Mittal, Aruna J Biomed Biotechnol Research Article Chlamydia trachomatis (CT) is the leading cause of diseases related to reproductive health and iron plays important role in chlamydial pathogenesis. Iron homeostasis in chlamydia-infected cells is not clear thus far. This study shows that expression of the transferrin receptor (TfR) is downregulated, whereas expression of the ferritin heavy chain is upregulated in CT-infected HeLa-229 cells. Expression of iron-regulatory protein (IRP)-1 predominates over IRP-2 in infected cells. In infected cells, attenuated binding activity of IRP-iron responsive elements (IREs) is observed using the electrophoretic mobility-shift assay. These results suggest that iron homeostasis is modulated in CT-infected HeLa cells at the interface of acquisition and commensal use of iron. Hindawi Publishing Corporation 2009 2009-08-16 /pmc/articles/PMC2727623/ /pubmed/19688112 http://dx.doi.org/10.1155/2009/342032 Text en Copyright © 2009 Harsh Vardhan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Vardhan, Harsh
Bhengraj, Apurb R.
Jha, Rajneesh
Singh Mittal, Aruna
Chlamydia trachomatis Alters Iron-Regulatory Protein-1 Binding Capacity and Modulates Cellular Iron Homeostasis in HeLa-229 Cells
title Chlamydia trachomatis Alters Iron-Regulatory Protein-1 Binding Capacity and Modulates Cellular Iron Homeostasis in HeLa-229 Cells
title_full Chlamydia trachomatis Alters Iron-Regulatory Protein-1 Binding Capacity and Modulates Cellular Iron Homeostasis in HeLa-229 Cells
title_fullStr Chlamydia trachomatis Alters Iron-Regulatory Protein-1 Binding Capacity and Modulates Cellular Iron Homeostasis in HeLa-229 Cells
title_full_unstemmed Chlamydia trachomatis Alters Iron-Regulatory Protein-1 Binding Capacity and Modulates Cellular Iron Homeostasis in HeLa-229 Cells
title_short Chlamydia trachomatis Alters Iron-Regulatory Protein-1 Binding Capacity and Modulates Cellular Iron Homeostasis in HeLa-229 Cells
title_sort chlamydia trachomatis alters iron-regulatory protein-1 binding capacity and modulates cellular iron homeostasis in hela-229 cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2727623/
https://www.ncbi.nlm.nih.gov/pubmed/19688112
http://dx.doi.org/10.1155/2009/342032
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