Cargando…
Dendritic Branching of Olfactory Bulb Mitral and Tufted Cells: Regulation by TrkB
BACKGROUND: Projection neurons of mammalian olfactory bulb (OB), mitral and tufted cells, have dendrites whose morphologies are specifically differentiated for efficient odor information processing. The apical dendrite extends radially and arborizes in single glomerulus where it receives primary inp...
Autores principales: | , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2727791/ https://www.ncbi.nlm.nih.gov/pubmed/19707543 http://dx.doi.org/10.1371/journal.pone.0006729 |
_version_ | 1782170699986632704 |
---|---|
author | Imamura, Fumiaki Greer, Charles A. |
author_facet | Imamura, Fumiaki Greer, Charles A. |
author_sort | Imamura, Fumiaki |
collection | PubMed |
description | BACKGROUND: Projection neurons of mammalian olfactory bulb (OB), mitral and tufted cells, have dendrites whose morphologies are specifically differentiated for efficient odor information processing. The apical dendrite extends radially and arborizes in single glomerulus where it receives primary input from olfactory sensory neurons that express the same odor receptor. The lateral dendrites extend horizontally in the external plexiform layer and make reciprocal dendrodendritic synapses with granule cells, which moderate mitral/tufted cell activity. The molecular mechanisms regulating dendritic development of mitral/tufted cells is one of the unsolved important problems in the olfactory system. Here, we focused on TrkB receptors to test the hypothesis that neurotrophin-mediate mechanisms contributed to dendritic differentiation of OB mitral/tufted cells. PRINCIPAL FINDINGS: With immunohistochemical analysis, we found that the TrkB neurotrophin receptor is expressed by both apical and lateral dendrites of mitral/tufted cells and that expression is evident during the early postnatal days when these dendrites exhibit their most robust growth and differentiation. To examine the effect of TrkB activation on mitral/tufted cell dendritic development, we cultured OB neurons. When BDNF or NT4 were introduced into the cultures, there was a significant increase in the number of primary neurites and branching points among the mitral/tufted cells. Moreover, BDNF facilitated filopodial extension along the neurites of mitral/tufted cells. SIGNIFICANCE: In this report, we show for the first time that TrkB activation stimulates the dendritic branching of mitral/tufted cells in developing OB. This suggests that arborization of the apical dendrite in a glomerulus is under the tight regulation of TrkB activation. |
format | Text |
id | pubmed-2727791 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-27277912009-08-25 Dendritic Branching of Olfactory Bulb Mitral and Tufted Cells: Regulation by TrkB Imamura, Fumiaki Greer, Charles A. PLoS One Research Article BACKGROUND: Projection neurons of mammalian olfactory bulb (OB), mitral and tufted cells, have dendrites whose morphologies are specifically differentiated for efficient odor information processing. The apical dendrite extends radially and arborizes in single glomerulus where it receives primary input from olfactory sensory neurons that express the same odor receptor. The lateral dendrites extend horizontally in the external plexiform layer and make reciprocal dendrodendritic synapses with granule cells, which moderate mitral/tufted cell activity. The molecular mechanisms regulating dendritic development of mitral/tufted cells is one of the unsolved important problems in the olfactory system. Here, we focused on TrkB receptors to test the hypothesis that neurotrophin-mediate mechanisms contributed to dendritic differentiation of OB mitral/tufted cells. PRINCIPAL FINDINGS: With immunohistochemical analysis, we found that the TrkB neurotrophin receptor is expressed by both apical and lateral dendrites of mitral/tufted cells and that expression is evident during the early postnatal days when these dendrites exhibit their most robust growth and differentiation. To examine the effect of TrkB activation on mitral/tufted cell dendritic development, we cultured OB neurons. When BDNF or NT4 were introduced into the cultures, there was a significant increase in the number of primary neurites and branching points among the mitral/tufted cells. Moreover, BDNF facilitated filopodial extension along the neurites of mitral/tufted cells. SIGNIFICANCE: In this report, we show for the first time that TrkB activation stimulates the dendritic branching of mitral/tufted cells in developing OB. This suggests that arborization of the apical dendrite in a glomerulus is under the tight regulation of TrkB activation. Public Library of Science 2009-08-25 /pmc/articles/PMC2727791/ /pubmed/19707543 http://dx.doi.org/10.1371/journal.pone.0006729 Text en Imamura, Greer. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Imamura, Fumiaki Greer, Charles A. Dendritic Branching of Olfactory Bulb Mitral and Tufted Cells: Regulation by TrkB |
title | Dendritic Branching of Olfactory Bulb Mitral and Tufted Cells: Regulation by TrkB |
title_full | Dendritic Branching of Olfactory Bulb Mitral and Tufted Cells: Regulation by TrkB |
title_fullStr | Dendritic Branching of Olfactory Bulb Mitral and Tufted Cells: Regulation by TrkB |
title_full_unstemmed | Dendritic Branching of Olfactory Bulb Mitral and Tufted Cells: Regulation by TrkB |
title_short | Dendritic Branching of Olfactory Bulb Mitral and Tufted Cells: Regulation by TrkB |
title_sort | dendritic branching of olfactory bulb mitral and tufted cells: regulation by trkb |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2727791/ https://www.ncbi.nlm.nih.gov/pubmed/19707543 http://dx.doi.org/10.1371/journal.pone.0006729 |
work_keys_str_mv | AT imamurafumiaki dendriticbranchingofolfactorybulbmitralandtuftedcellsregulationbytrkb AT greercharlesa dendriticbranchingofolfactorybulbmitralandtuftedcellsregulationbytrkb |