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Identification of Genes Responsive to Solar Simulated UV Radiation in Human Monocyte-Derived Dendritic Cells

Ultraviolet (UV) irradiation has profound effects on the skin and the systemic immune system. Several effects of UV radiation on Dendritic cells (DCs) functions have been described. However, gene expression changes induced by UV radiation in DCs have not been addressed before. In this report, we irr...

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Autores principales: de la Fuente, Hortensia, Lamana, Amalia, Mittelbrunn, María, Perez-Gala, Silvia, Gonzalez, Salvador, García-Diez, Amaro, Vega, Miguel, Sanchez-Madrid, Francisco
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2727914/
https://www.ncbi.nlm.nih.gov/pubmed/19707549
http://dx.doi.org/10.1371/journal.pone.0006735
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author de la Fuente, Hortensia
Lamana, Amalia
Mittelbrunn, María
Perez-Gala, Silvia
Gonzalez, Salvador
García-Diez, Amaro
Vega, Miguel
Sanchez-Madrid, Francisco
author_facet de la Fuente, Hortensia
Lamana, Amalia
Mittelbrunn, María
Perez-Gala, Silvia
Gonzalez, Salvador
García-Diez, Amaro
Vega, Miguel
Sanchez-Madrid, Francisco
author_sort de la Fuente, Hortensia
collection PubMed
description Ultraviolet (UV) irradiation has profound effects on the skin and the systemic immune system. Several effects of UV radiation on Dendritic cells (DCs) functions have been described. However, gene expression changes induced by UV radiation in DCs have not been addressed before. In this report, we irradiated human monocyte-derived DCs with solar-simulated UVA/UVB and analyzed regulated genes on human whole genome arrays. Results were validated by RT-PCR and further analyzed by Gene Set Enrichment Analysis (GSEA). Solar-simulated UV radiation up-regulated expression of genes involved in cellular stress and inflammation, and down-regulated genes involved in chemotaxis, vesicular transport and RNA processing. Twenty four genes were selected for comparison by RT-PCR with similarly treated human primary keratinocytes and human melanocytes. Several genes involved in the regulation of the immune response were differentially regulated in UVA/UVB irradiated human monocyte-derived DCs, such as protein tyrosine phosphatase, receptor type E (PTPRE), thrombospondin-1 (THBS1), inducible costimulator ligand (ICOSL), galectins, Src-like adapter protein (SLA), IL-10 and CCR7. These results indicate that UV-exposure triggers the regulation of a complex gene repertoire involved in human-DC–mediated immune responses.
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spelling pubmed-27279142009-08-26 Identification of Genes Responsive to Solar Simulated UV Radiation in Human Monocyte-Derived Dendritic Cells de la Fuente, Hortensia Lamana, Amalia Mittelbrunn, María Perez-Gala, Silvia Gonzalez, Salvador García-Diez, Amaro Vega, Miguel Sanchez-Madrid, Francisco PLoS One Research Article Ultraviolet (UV) irradiation has profound effects on the skin and the systemic immune system. Several effects of UV radiation on Dendritic cells (DCs) functions have been described. However, gene expression changes induced by UV radiation in DCs have not been addressed before. In this report, we irradiated human monocyte-derived DCs with solar-simulated UVA/UVB and analyzed regulated genes on human whole genome arrays. Results were validated by RT-PCR and further analyzed by Gene Set Enrichment Analysis (GSEA). Solar-simulated UV radiation up-regulated expression of genes involved in cellular stress and inflammation, and down-regulated genes involved in chemotaxis, vesicular transport and RNA processing. Twenty four genes were selected for comparison by RT-PCR with similarly treated human primary keratinocytes and human melanocytes. Several genes involved in the regulation of the immune response were differentially regulated in UVA/UVB irradiated human monocyte-derived DCs, such as protein tyrosine phosphatase, receptor type E (PTPRE), thrombospondin-1 (THBS1), inducible costimulator ligand (ICOSL), galectins, Src-like adapter protein (SLA), IL-10 and CCR7. These results indicate that UV-exposure triggers the regulation of a complex gene repertoire involved in human-DC–mediated immune responses. Public Library of Science 2009-08-26 /pmc/articles/PMC2727914/ /pubmed/19707549 http://dx.doi.org/10.1371/journal.pone.0006735 Text en de la Fuente et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
de la Fuente, Hortensia
Lamana, Amalia
Mittelbrunn, María
Perez-Gala, Silvia
Gonzalez, Salvador
García-Diez, Amaro
Vega, Miguel
Sanchez-Madrid, Francisco
Identification of Genes Responsive to Solar Simulated UV Radiation in Human Monocyte-Derived Dendritic Cells
title Identification of Genes Responsive to Solar Simulated UV Radiation in Human Monocyte-Derived Dendritic Cells
title_full Identification of Genes Responsive to Solar Simulated UV Radiation in Human Monocyte-Derived Dendritic Cells
title_fullStr Identification of Genes Responsive to Solar Simulated UV Radiation in Human Monocyte-Derived Dendritic Cells
title_full_unstemmed Identification of Genes Responsive to Solar Simulated UV Radiation in Human Monocyte-Derived Dendritic Cells
title_short Identification of Genes Responsive to Solar Simulated UV Radiation in Human Monocyte-Derived Dendritic Cells
title_sort identification of genes responsive to solar simulated uv radiation in human monocyte-derived dendritic cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2727914/
https://www.ncbi.nlm.nih.gov/pubmed/19707549
http://dx.doi.org/10.1371/journal.pone.0006735
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