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GASZ Is Essential for Male Meiosis and Suppression of Retrotransposon Expression in the Male Germline
Nuage are amorphous ultrastructural granules in the cytoplasm of male germ cells as divergent as Drosophila, Xenopus, and Homo sapiens. Most nuage are cytoplasmic ribonucleoprotein structures implicated in diverse RNA metabolism including the regulation of PIWI-interacting RNA (piRNA) synthesis by t...
Autores principales: | , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2727916/ https://www.ncbi.nlm.nih.gov/pubmed/19730684 http://dx.doi.org/10.1371/journal.pgen.1000635 |
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author | Ma, Lang Buchold, Gregory M. Greenbaum, Michael P. Roy, Angshumoy Burns, Kathleen H. Zhu, Huifeng Han, Derek Y. Harris, R. Alan Coarfa, Cristian Gunaratne, Preethi H. Yan, Wei Matzuk, Martin M. |
author_facet | Ma, Lang Buchold, Gregory M. Greenbaum, Michael P. Roy, Angshumoy Burns, Kathleen H. Zhu, Huifeng Han, Derek Y. Harris, R. Alan Coarfa, Cristian Gunaratne, Preethi H. Yan, Wei Matzuk, Martin M. |
author_sort | Ma, Lang |
collection | PubMed |
description | Nuage are amorphous ultrastructural granules in the cytoplasm of male germ cells as divergent as Drosophila, Xenopus, and Homo sapiens. Most nuage are cytoplasmic ribonucleoprotein structures implicated in diverse RNA metabolism including the regulation of PIWI-interacting RNA (piRNA) synthesis by the PIWI family (i.e., MILI, MIWI2, and MIWI). MILI is prominent in embryonic and early post-natal germ cells in nuage also called germinal granules that are often associated with mitochondria and called intermitochondrial cement. We find that GASZ (Germ cell protein with Ankyrin repeats, Sterile alpha motif, and leucine Zipper) co-localizes with MILI in intermitochondrial cement. Knockout of Gasz in mice results in a dramatic downregulation of MILI, and phenocopies the zygotene–pachytene spermatocyte block and male sterility defect observed in MILI null mice. In Gasz null testes, we observe increased hypomethylation and expression of retrotransposons similar to MILI null testes. We also find global shifts in the small RNAome, including down-regulation of repeat-associated, known, and novel piRNAs. These studies provide the first evidence for an essential structural role for GASZ in male fertility and epigenetic and post-transcriptional silencing of retrotransposons by stabilizing MILI in nuage. |
format | Text |
id | pubmed-2727916 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-27279162009-09-04 GASZ Is Essential for Male Meiosis and Suppression of Retrotransposon Expression in the Male Germline Ma, Lang Buchold, Gregory M. Greenbaum, Michael P. Roy, Angshumoy Burns, Kathleen H. Zhu, Huifeng Han, Derek Y. Harris, R. Alan Coarfa, Cristian Gunaratne, Preethi H. Yan, Wei Matzuk, Martin M. PLoS Genet Research Article Nuage are amorphous ultrastructural granules in the cytoplasm of male germ cells as divergent as Drosophila, Xenopus, and Homo sapiens. Most nuage are cytoplasmic ribonucleoprotein structures implicated in diverse RNA metabolism including the regulation of PIWI-interacting RNA (piRNA) synthesis by the PIWI family (i.e., MILI, MIWI2, and MIWI). MILI is prominent in embryonic and early post-natal germ cells in nuage also called germinal granules that are often associated with mitochondria and called intermitochondrial cement. We find that GASZ (Germ cell protein with Ankyrin repeats, Sterile alpha motif, and leucine Zipper) co-localizes with MILI in intermitochondrial cement. Knockout of Gasz in mice results in a dramatic downregulation of MILI, and phenocopies the zygotene–pachytene spermatocyte block and male sterility defect observed in MILI null mice. In Gasz null testes, we observe increased hypomethylation and expression of retrotransposons similar to MILI null testes. We also find global shifts in the small RNAome, including down-regulation of repeat-associated, known, and novel piRNAs. These studies provide the first evidence for an essential structural role for GASZ in male fertility and epigenetic and post-transcriptional silencing of retrotransposons by stabilizing MILI in nuage. Public Library of Science 2009-09-04 /pmc/articles/PMC2727916/ /pubmed/19730684 http://dx.doi.org/10.1371/journal.pgen.1000635 Text en Ma et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Ma, Lang Buchold, Gregory M. Greenbaum, Michael P. Roy, Angshumoy Burns, Kathleen H. Zhu, Huifeng Han, Derek Y. Harris, R. Alan Coarfa, Cristian Gunaratne, Preethi H. Yan, Wei Matzuk, Martin M. GASZ Is Essential for Male Meiosis and Suppression of Retrotransposon Expression in the Male Germline |
title | GASZ Is Essential for Male Meiosis and Suppression of Retrotransposon Expression in the Male Germline |
title_full | GASZ Is Essential for Male Meiosis and Suppression of Retrotransposon Expression in the Male Germline |
title_fullStr | GASZ Is Essential for Male Meiosis and Suppression of Retrotransposon Expression in the Male Germline |
title_full_unstemmed | GASZ Is Essential for Male Meiosis and Suppression of Retrotransposon Expression in the Male Germline |
title_short | GASZ Is Essential for Male Meiosis and Suppression of Retrotransposon Expression in the Male Germline |
title_sort | gasz is essential for male meiosis and suppression of retrotransposon expression in the male germline |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2727916/ https://www.ncbi.nlm.nih.gov/pubmed/19730684 http://dx.doi.org/10.1371/journal.pgen.1000635 |
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