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Zebrafish reward mutants reveal novel transcripts mediating the behavioral effects of amphetamine

BACKGROUND: Addiction is a pathological dysregulation of the brain's reward systems, determined by several complex genetic pathways. The conditioned place preference test provides an evaluation of the effects of drugs in animal models, allowing the investigation of substances at a biologically...

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Autores principales: Webb, Katharine J, Norton, William HJ, Trümbach, Dietrich, Meijer, Annemarie H, Ninkovic, Jovica, Topp, Stefanie, Heck, Daniel, Marr, Carsten, Wurst, Wolfgang, Theis, Fabian J, Spaink, Herman P, Bally-Cuif, Laure
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2728535/
https://www.ncbi.nlm.nih.gov/pubmed/19646228
http://dx.doi.org/10.1186/gb-2009-10-7-r81
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author Webb, Katharine J
Norton, William HJ
Trümbach, Dietrich
Meijer, Annemarie H
Ninkovic, Jovica
Topp, Stefanie
Heck, Daniel
Marr, Carsten
Wurst, Wolfgang
Theis, Fabian J
Spaink, Herman P
Bally-Cuif, Laure
author_facet Webb, Katharine J
Norton, William HJ
Trümbach, Dietrich
Meijer, Annemarie H
Ninkovic, Jovica
Topp, Stefanie
Heck, Daniel
Marr, Carsten
Wurst, Wolfgang
Theis, Fabian J
Spaink, Herman P
Bally-Cuif, Laure
author_sort Webb, Katharine J
collection PubMed
description BACKGROUND: Addiction is a pathological dysregulation of the brain's reward systems, determined by several complex genetic pathways. The conditioned place preference test provides an evaluation of the effects of drugs in animal models, allowing the investigation of substances at a biologically relevant level with respect to reward. Our lab has previously reported the development of a reliable conditioned place preference paradigm for zebrafish. Here, this test was used to isolate a dominant N-ethyl-N-nitrosourea (ENU)-induced mutant, no addiction (nad(dne3256)), which fails to respond to amphetamine, and which we used as an entry point towards identifying the behaviorally relevant transcriptional response to amphetamine. RESULTS: Through the combination of microarray experiments comparing the adult brain transcriptome of mutant and wild-type siblings under normal conditions, as well as their response to amphetamine, we identified genes that correlate with the mutants' altered conditioned place preference behavior. In addition to pathways classically involved in reward, this gene set shows a striking enrichment in transcription factor-encoding genes classically involved in brain development, which later appear to be re-used within the adult brain. We selected a subset of them for validation by quantitative PCR and in situ hybridization, revealing that specific brain areas responding to the drug through these transcription factors include domains of ongoing adult neurogenesis. Finally, network construction revealed functional connections between several of these genes. CONCLUSIONS: Together, our results identify a new network of coordinated gene regulation that influences or accompanies amphetamine-triggered conditioned place preference behavior and that may underlie the susceptibility to addiction.
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spelling pubmed-27285352009-08-18 Zebrafish reward mutants reveal novel transcripts mediating the behavioral effects of amphetamine Webb, Katharine J Norton, William HJ Trümbach, Dietrich Meijer, Annemarie H Ninkovic, Jovica Topp, Stefanie Heck, Daniel Marr, Carsten Wurst, Wolfgang Theis, Fabian J Spaink, Herman P Bally-Cuif, Laure Genome Biol Research BACKGROUND: Addiction is a pathological dysregulation of the brain's reward systems, determined by several complex genetic pathways. The conditioned place preference test provides an evaluation of the effects of drugs in animal models, allowing the investigation of substances at a biologically relevant level with respect to reward. Our lab has previously reported the development of a reliable conditioned place preference paradigm for zebrafish. Here, this test was used to isolate a dominant N-ethyl-N-nitrosourea (ENU)-induced mutant, no addiction (nad(dne3256)), which fails to respond to amphetamine, and which we used as an entry point towards identifying the behaviorally relevant transcriptional response to amphetamine. RESULTS: Through the combination of microarray experiments comparing the adult brain transcriptome of mutant and wild-type siblings under normal conditions, as well as their response to amphetamine, we identified genes that correlate with the mutants' altered conditioned place preference behavior. In addition to pathways classically involved in reward, this gene set shows a striking enrichment in transcription factor-encoding genes classically involved in brain development, which later appear to be re-used within the adult brain. We selected a subset of them for validation by quantitative PCR and in situ hybridization, revealing that specific brain areas responding to the drug through these transcription factors include domains of ongoing adult neurogenesis. Finally, network construction revealed functional connections between several of these genes. CONCLUSIONS: Together, our results identify a new network of coordinated gene regulation that influences or accompanies amphetamine-triggered conditioned place preference behavior and that may underlie the susceptibility to addiction. BioMed Central 2009 2009-07-31 /pmc/articles/PMC2728535/ /pubmed/19646228 http://dx.doi.org/10.1186/gb-2009-10-7-r81 Text en Copyright © 2009 Webb et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Webb, Katharine J
Norton, William HJ
Trümbach, Dietrich
Meijer, Annemarie H
Ninkovic, Jovica
Topp, Stefanie
Heck, Daniel
Marr, Carsten
Wurst, Wolfgang
Theis, Fabian J
Spaink, Herman P
Bally-Cuif, Laure
Zebrafish reward mutants reveal novel transcripts mediating the behavioral effects of amphetamine
title Zebrafish reward mutants reveal novel transcripts mediating the behavioral effects of amphetamine
title_full Zebrafish reward mutants reveal novel transcripts mediating the behavioral effects of amphetamine
title_fullStr Zebrafish reward mutants reveal novel transcripts mediating the behavioral effects of amphetamine
title_full_unstemmed Zebrafish reward mutants reveal novel transcripts mediating the behavioral effects of amphetamine
title_short Zebrafish reward mutants reveal novel transcripts mediating the behavioral effects of amphetamine
title_sort zebrafish reward mutants reveal novel transcripts mediating the behavioral effects of amphetamine
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2728535/
https://www.ncbi.nlm.nih.gov/pubmed/19646228
http://dx.doi.org/10.1186/gb-2009-10-7-r81
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