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Evaluation of conjunctival inflammatory status by confocal scanning laser microscopy and conjunctival brush cytology in patients with atopic keratoconjunctivitis (AKC)
PURPOSE: To elucidate the status of the conjunctival inflammation in atopic keratoconjunctivitis (AKC) using laser scanning confocal microscopy and compare the relevant findings with conjunctival brush cytology in a prospective controlled study. METHODS: Twenty eyes from 20 AKC patients as well as 1...
Autores principales: | , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Molecular Vision
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2728562/ https://www.ncbi.nlm.nih.gov/pubmed/19693288 |
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author | Wakamatsu, Tais Hitomi Okada, Naoko Kojima, Takashi Matsumoto, Yukihiro Ibrahim, Osama M.A. Dogru, Murat Adan, Enrique Sato Fukagawa, Kazumi Katakami, Chikako Tsubota, Kazuo Shimazaki, Jun Fujishima, Hiroshi |
author_facet | Wakamatsu, Tais Hitomi Okada, Naoko Kojima, Takashi Matsumoto, Yukihiro Ibrahim, Osama M.A. Dogru, Murat Adan, Enrique Sato Fukagawa, Kazumi Katakami, Chikako Tsubota, Kazuo Shimazaki, Jun Fujishima, Hiroshi |
author_sort | Wakamatsu, Tais Hitomi |
collection | PubMed |
description | PURPOSE: To elucidate the status of the conjunctival inflammation in atopic keratoconjunctivitis (AKC) using laser scanning confocal microscopy and compare the relevant findings with conjunctival brush cytology in a prospective controlled study. METHODS: Twenty eyes from 20 AKC patients as well as 16 eyes from 16 age and sex matched normal subjects were studied. The subjects underwent tear film break-up time (BUT), fluorescein and Rose Bengal staining of the ocular surface, conjunctival confocal microscopy, Schirmer test, and brush cytology. Brush cytology specimens and in vivo confocal microscopy scans underwent evaluation for inflammatory cell densities. RESULTS: Brush cytology specimens and in vivo confocal microscopy scans from AKC patients revealed significantly higher numbers of inflammatory cells (p<0.05). Conjunctival inflammatory cell density showed a negative correlation with tear stability and a positive correlation with vital staining scores and conjunctival injection grades. The extent of conjunctival inflammation assessed by in vivo confocal microscopy showed a strong positive linear correlation with the inflammation status evaluated by brush cytology. The corneal inflammatory cell density assessed by in vivo confocal microscopy showed a significant negative correlation with tear stability and a positive linear correlation with corneal fluorescein staining. CONCLUSIONS: Confocal scanning laser microscopy is an efficient, noninvasive, and a promising tool for the quantitative assessment of conjunctival inflammation, a parameter of this new technology which correlated well with subjective and objective ocular surface clinical findings. |
format | Text |
id | pubmed-2728562 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Molecular Vision |
record_format | MEDLINE/PubMed |
spelling | pubmed-27285622009-08-19 Evaluation of conjunctival inflammatory status by confocal scanning laser microscopy and conjunctival brush cytology in patients with atopic keratoconjunctivitis (AKC) Wakamatsu, Tais Hitomi Okada, Naoko Kojima, Takashi Matsumoto, Yukihiro Ibrahim, Osama M.A. Dogru, Murat Adan, Enrique Sato Fukagawa, Kazumi Katakami, Chikako Tsubota, Kazuo Shimazaki, Jun Fujishima, Hiroshi Mol Vis Research Article PURPOSE: To elucidate the status of the conjunctival inflammation in atopic keratoconjunctivitis (AKC) using laser scanning confocal microscopy and compare the relevant findings with conjunctival brush cytology in a prospective controlled study. METHODS: Twenty eyes from 20 AKC patients as well as 16 eyes from 16 age and sex matched normal subjects were studied. The subjects underwent tear film break-up time (BUT), fluorescein and Rose Bengal staining of the ocular surface, conjunctival confocal microscopy, Schirmer test, and brush cytology. Brush cytology specimens and in vivo confocal microscopy scans underwent evaluation for inflammatory cell densities. RESULTS: Brush cytology specimens and in vivo confocal microscopy scans from AKC patients revealed significantly higher numbers of inflammatory cells (p<0.05). Conjunctival inflammatory cell density showed a negative correlation with tear stability and a positive correlation with vital staining scores and conjunctival injection grades. The extent of conjunctival inflammation assessed by in vivo confocal microscopy showed a strong positive linear correlation with the inflammation status evaluated by brush cytology. The corneal inflammatory cell density assessed by in vivo confocal microscopy showed a significant negative correlation with tear stability and a positive linear correlation with corneal fluorescein staining. CONCLUSIONS: Confocal scanning laser microscopy is an efficient, noninvasive, and a promising tool for the quantitative assessment of conjunctival inflammation, a parameter of this new technology which correlated well with subjective and objective ocular surface clinical findings. Molecular Vision 2009-08-15 /pmc/articles/PMC2728562/ /pubmed/19693288 Text en http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wakamatsu, Tais Hitomi Okada, Naoko Kojima, Takashi Matsumoto, Yukihiro Ibrahim, Osama M.A. Dogru, Murat Adan, Enrique Sato Fukagawa, Kazumi Katakami, Chikako Tsubota, Kazuo Shimazaki, Jun Fujishima, Hiroshi Evaluation of conjunctival inflammatory status by confocal scanning laser microscopy and conjunctival brush cytology in patients with atopic keratoconjunctivitis (AKC) |
title | Evaluation of conjunctival inflammatory status by confocal scanning laser microscopy and conjunctival brush cytology in patients with atopic keratoconjunctivitis (AKC) |
title_full | Evaluation of conjunctival inflammatory status by confocal scanning laser microscopy and conjunctival brush cytology in patients with atopic keratoconjunctivitis (AKC) |
title_fullStr | Evaluation of conjunctival inflammatory status by confocal scanning laser microscopy and conjunctival brush cytology in patients with atopic keratoconjunctivitis (AKC) |
title_full_unstemmed | Evaluation of conjunctival inflammatory status by confocal scanning laser microscopy and conjunctival brush cytology in patients with atopic keratoconjunctivitis (AKC) |
title_short | Evaluation of conjunctival inflammatory status by confocal scanning laser microscopy and conjunctival brush cytology in patients with atopic keratoconjunctivitis (AKC) |
title_sort | evaluation of conjunctival inflammatory status by confocal scanning laser microscopy and conjunctival brush cytology in patients with atopic keratoconjunctivitis (akc) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2728562/ https://www.ncbi.nlm.nih.gov/pubmed/19693288 |
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